Apparatus for non-invasive in vivo measurement by raman spectroscopy

• US 9,380,942 B2
• Filed: 07/05/2012
• Issued: 07/05/2016
• Est. Priority Date: 01/07/2010
• Status: Active Grant

• Current Assignee: Rsp Systems A/S
• Original Assignee: Rsp Systems A/S
• Inventors: Henneberg, Morten, Banke, Stefan Ovesen, Weber, Anders
• Primary Examiner(s): Winakur, Eric
• Assistant Examiner(s): Fardanesh, Marjan
• Application Number:
  US13/542,462
• Publication Number:
  US 20130018237A1
• Time in Patent Office: 1,461 Days
• Field of Search: 600310-344
• US Class Current: 1/1
• CPC Class Codes: A61B 2562/0238 Optical sensor arrangements for performing transmission measurements on body tissue
• CPC Class Codes: A61B 2562/0242 for varying or adjusting the optical path length in the tissue
• CPC Class Codes: A61B 2562/146 for optical coupling
• CPC Class Codes: A61B 5/0059 using light, e.g. diagnosis by transillumination, diascopy, fluorescence A61B5/0093, A61B5/1455, A61B5/02416 and A61B5/0261 take precedence
• CPC Class Codes: A61B 5/0068 Confocal scanning
• CPC Class Codes: A61B 5/0075 by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy A61B5/0071 takes precedence
• CPC Class Codes: A61B 5/1451 for interstitial fluid
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/1455 using optical sensors, e.g. spectral photometrical oximeters
• CPC Class Codes: A61B 5/1495 Calibrating or testing of in-vivo probes
• CPC Class Codes: A61B 5/441 Skin evaluation, e.g. for skin disorder diagnosis
• CPC Class Codes: G01N 21/65 Raman scattering

Abstract

A metabolite concentration is measured in vivo using Raman spectroscopy in such a way as to receive at a detector (229) light scattered from the metabolite in interstitial fluid in skin in a measurement location (217) at a depth (218) of from 200-300 μm below the skin surface providing improved retention of correct calibration and transferability of calibration between individual subjects.

Claims

1. A method for non-invasive in vivo measurement by Raman spectroscopy of glucose present in interstitial fluid in the skin of a subject, comprising directing light from a light source into the skin via optical components defining a light path from said light source to a measurement location in the skin at a depth located at from 200 to 300 μ

m beneath the surface of the skin, receiving Raman scattered light back from the skin at a light detection unit via optical components defining a return path including using a skin engaging member having a distal surface for defining the position of said optical components defining the return path with respect to a surface of said skin and using said optical components defining a return path for Raman scattered light to transmit selectively to said light detection unit Raman scattered light scattered from near said measurement location of which at least 50% originates at depths from 60 to 400 μ

m beyond said distal surface of the skin engaging member, and deriving a measurement of glucose concentration at said measuring location from the Raman scattered light received at the light detection unit.

2. A method as claimed in claim 1, wherein the percentage of light transmitted by said optical components defining a return path for Raman scattered light that originates at a range of depths 60 to 400 μ

m beyond said distal surface of the skin engaging member, is at least 55%.

3. A method as claimed in claim 2, wherein the percentage of Raman scattered light transmitted by said optical components acting to transmit to said light detection unit light scattered from near said measurement location that originates at depths less than 600 μ

m beyond said distal surface of the skin engaging member is at least 90%.

4. A method as claimed in claim 1, wherein said light source is a substantially monochromatic light source emitting a wavelength selected from the group consisting of 785, 830, and 850 nm.

5. A method as claimed in claim 1, performed using a hand piece for application to the skin containing components defining said measurement location in use, and one or more optical fibres connecting said hand piece to said light source and to a processing unit containing electronic circuitry for analysis of signals received from said light detection unit to provide said measurement therefrom.

6. A method as claimed in claim 1, wherein the optical components defining a light path from said light source to a measurement location and the skin engaging member are configured such that the position distal of the skin engaging member, at which the light emitted from the light source is focused, is adjustable.

7. A method as claimed in claim 1, wherein the optical components defining a light path from said light source to a measurement location and the skin engaging member are configured such that the position distal of the skin engaging member, at which the light emitted from the light source is focused of said measurement location, is fixed.