Apparatus for loading molecules onto substrates
First Claim
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1. An apparatus comprising:
- a fixture for holding a substrate;
a substrate held within the fixture comprising an array of zero mode waveguides on its top surface,a reservoir for containing a solution in contact with the top surface of the substrate;
a solution, in contact with the substrate, comprising magnetic beads having molecules-of-interest attached thereto, the magnetic beads having diameters greater than the smallest cross-sectional dimensions of the zero mode waveguides; and
a device for generating a dynamic magnetic field disposed below, adjacent to, or above the substrate capable of generating a magnetic field that (i) pulls the magnetic beads to the top surface of the substrate, and (ii) moves the magnetic beads across the top surface of the substrate, whereby molecules-of-interest are deposited within the zero mode waveguides on the substrate.
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Abstract
Disclosed are compositions, devices, and methods for loading molecules of interest onto a substrate by contacting beads having molecules of interest attached to them with the substrate, for example by providing a field that brings the beads into proximity or contact with the substrate and moves the beads with respect to the substrate. Such molecules of interest can be deposited onto substrates for single-molecule analysis.
64 Citations
20 Claims
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1. An apparatus comprising:
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a fixture for holding a substrate; a substrate held within the fixture comprising an array of zero mode waveguides on its top surface, a reservoir for containing a solution in contact with the top surface of the substrate; a solution, in contact with the substrate, comprising magnetic beads having molecules-of-interest attached thereto, the magnetic beads having diameters greater than the smallest cross-sectional dimensions of the zero mode waveguides; and a device for generating a dynamic magnetic field disposed below, adjacent to, or above the substrate capable of generating a magnetic field that (i) pulls the magnetic beads to the top surface of the substrate, and (ii) moves the magnetic beads across the top surface of the substrate, whereby molecules-of-interest are deposited within the zero mode waveguides on the substrate. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification