System and method for automated sample preparation

• US 9,381,524 B2
• Filed: 11/08/2012
• Issued: 07/05/2016
• Est. Priority Date: 11/08/2011
• Status: Active Grant

• Current Assignee: Becton, Dickinson & Co
• Original Assignee: Becton, Dickinson & Co
• Inventors: Embres, Christopher, Gorelick, Donald, Zhou, Tong, Bailey, Kevin, Hansen, Timothy R., Livingston, Dwight, Scherz, Andre, Sevigny, Gerard, Talmer, Mark, Lovette, Spencer
• Primary Examiner(s): Cleveland, Timothy
• Application Number:
  US13/672,302
• Publication Number:
  US 20130116102A1
• Time in Patent Office: 1,335 Days
• US Class Current: 1/1
• CPC Class Codes: B01D 43/00 Separating particles from liquids, or liquids from solids, otherwise than by sedimentation or filtration flotation processes B03D1/00; drying solid materials or objects F26B
• CPC Class Codes: B04B 11/043 Load indication with or without control arrangements
• CPC Class Codes: B04B 5/0414 comprising test tubes
• CPC Class Codes: G01N 2035/00495 Centrifuges
• CPC Class Codes: G01N 2035/0405 manipulating closing or opening means, e.g. stoppers, screw caps, lids or covers
• CPC Class Codes: G01N 2035/0425 Stacks, magazines or elevators for plates
• CPC Class Codes: G01N 2035/0465 Loading or unloading the conveyor
• CPC Class Codes: G01N 35/026 having blocks or racks of reaction cells or cuvettes
• CPC Class Codes: G01N 35/04 Details of the conveyor system G01N35/021 - G01N35/028 take precedence

Abstract

A system for automatically processing a biological specimen is provided that includes an elevator comprising a plurality of shelves configured to receive a plurality of sample trays. The trays may comprise a plurality of sample containers containing a sample and having a plurality of respective caps engaged therewith. The trays may further include a plurality of centrifuge tube racks each containing a plurality of centrifuge tubes. The system may include a first transport mechanism, a second transport mechanism and a third transport mechanism. The system may include a chain-of-custody device configured to read identifiers on each of the containers. The system may also include a pipetting device configured to remove a portion from the sample containers and dispense the sample into the centrifuge tubes.

Claims

1. A system for automated sample preparation comprising:

an elevator comprising a plurality of shelves configured to receive a plurality of trays, each of the trays comprising;

a plurality of sample containers containing a sample and having a plurality of respective caps engaged therewith; and

a plurality of centrifuge tube racks each containing a plurality of centrifuge tubes;

a first transport mechanism configured to transport the plurality of trays, one at a time, from the elevator to a processing deck;

a second transport mechanism coupled to the processing deck and configured to transport the processing deck from a location external to the elevator reachable by the first transport mechanism to a first processing location and between the first processing location and a second processing location, wherein the first transport mechanism is configured to transport each one of the plurality of trays in at least one axis not used by the second transport mechanism;

a chain-of-custody device configured to read an identifier on each of the sample containers;

a de-capping device configured to remove the plurality of caps from the plurality of respective sample containers;

a pipetting device configured to remove a portion of the sample from each of the plurality of sample containers having the plurality of respective caps disengaged and dispense the removed portion into the plurality of centrifuge tubes for each of the plurality of centrifuge tube racks;

a centrifuge device configured to centrifuge the plurality of the centrifuge tubes for each of the plurality of centrifuge tube racks containing the sample; and

a third transport mechanism configured to transport each of the centrifuge tube racks from one of the plurality of trays to the centrifuge device.

2. The system of claim 1, wherein each of the trays comprises a plurality of molecular tubes having a plurality of respective caps engaged therewith.

3. The system of claim 2, wherein the de-capping device is configured to remove the plurality of caps from the molecular tubes.

4. The system of claim 3, wherein the pipetting device is configured to remove a portion of the sample from each of the de-capped plurality of containers and dispense the removed portion into a plurality of de-capped molecular tubes.

5. The system of claim 1, wherein the de-capping device is configured to remove the plurality of caps while the plurality of sample containers are disposed within one of the plurality of trays.

6. The system of claim 1, wherein the third transport mechanism is configured to transport centrifuge tubes from the centrifuge device to an aspirating and decanting device following a centrifuge operation for aspirating or decanting centrifuge tubes.

7. The system of claim 1, wherein the first transport mechanism is configured to engage a bottom surface of each one of the plurality of trays, one at a time, and move the tray in both an X-direction and a Z-direction.

8. The system of claim 1, wherein the second transport mechanism is configured to convey the processing deck between the first processing location and the second processing location along a Y-direction.

9. The system of claim 1, wherein the third transport mechanism is configured to transport the plurality of centrifuge tubes from the tray at the second processing location to the centrifuge device in both an X-direction and a Z-direction.

10. The system of claim 1, wherein following centrifugation, the third transport mechanism is configured to transport the plurality of centrifuge tube racks from the centrifuge device to one of the plurality of trays at the second processing location, the second transport mechanism is configured to transport one of the plurality of trays from the second processing location to the location external to the elevator reachable by the first transport mechanism, and the first transport mechanism is configured to transport one of the plurality of trays from the processing deck into the elevator.

11. The system of claim 1, wherein each of the centrifuge tube racks is disposed within each of the plurality of trays at an angle of between about 13 and about 15 degrees relative to an axis transverse to a bottom surface of each of the plurality of trays.

12. The system of claim 1, wherein the second transport mechanism is configured to transport the processing deck between the first processing location and the second processing location to facilitate access to each of the centrifuge tube racks and centrifuge tubes with the third transport mechanism.

13. The system of claim 1, wherein the chain-of-custody device is configured to read an identifier on each of the sample containers at the first processing location.

14. The system of claim 1, further comprising a second chain-of-custody device configured to read an identifier on each of the trays and centrifuge tube racks in the elevator.

15. The system of claim 14, wherein the second chain-of-custody device is configured to determine whether each of the centrifuge tubes and centrifuge tube racks are properly positioned within each of the plurality of trays.

16. The system of claim 15, further comprising a third chain-of-custody device configured to read an identifier on each of the centrifuge tubes.

17. The system of claim 1, wherein each one of the plurality of trays comprises a plurality of disposable syringes, and wherein the pipetting device is configured to engage the plurality of disposable syringes and remove a portion of the sample from each of the de-capped plurality of sample containers with the syringes.

18. The system of claim 17, wherein the pipetting device is configured to disengage the plurality of disposable syringes following use.

19. The system of claim 1, wherein the pipetting device is configured to mix the sample in each of the de-capped plurality of containers prior to removing the portion of the sample from the sample containers.

20. The system of claim 1, wherein the de-capping device is configured to simultaneously remove the plurality of caps from the plurality of respective sample containers at the first processing location.

21. The system of claim 1, wherein the pipetting device is configured to simultaneously remove a portion of the sample from each of the de-capped plurality of containers and simultaneously dispense the removed portion into the plurality of centrifuge tubes for each of the plurality of centrifuge tube racks.

22. The system of claim 1, wherein the elevator comprises a plurality of shelves spaced vertically apart from one another, each shelf configured to support at least one of the plurality of trays thereon.