Fast detection of the presence of a target microbe in a liquid sample

• US 9,382,569 B1
• Filed: 01/16/2013
• Issued: 07/05/2016
• Est. Priority Date: 01/17/2012
• Status: Active Grant

• Current Assignee: Elemental Scientific Incorporated
• Original Assignee: Elemental Scientific Incorporated
• Inventors: Wiederin, Daniel R.
• Primary Examiner(s): Bowers, Nathan
• Application Number:
  US13/742,433
• Time in Patent Office: 1,266 Days
• US Class Current: 1/1
• CPC Class Codes: C12Q 1/04 Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor C12Q1/6897 takes precedence
• CPC Class Codes: C12Q 1/10 Enterobacteria
• CPC Class Codes: G01N 2021/7786 Fluorescence
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/77 by observing the effect on a chemical indicator
• CPC Class Codes: G01N 35/00 Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor

Abstract

A system is provided that includes a tray having a number of compartments for holding liquid samples and partitioning the liquid samples from one another. The liquid samples are prepared by adding an indicator configured to produce a characteristic change in light from the liquid samples when a target microbe metabolizes the indicator while the liquid samples are incubated. The system also includes a light sensor for sensing light from the liquid samples held in the tray while the plurality of liquid samples is incubated. The system further includes a processor coupled with the light sensor and configured to analyze the light from the liquid samples while the liquid samples are incubated to detect the characteristic change in light from one or more of the liquid samples if the target microbe is present in the liquid samples.

Claims

1. A system for detecting the presence of a target microbe comprising:

a tray configured to be received in an incubator for holding liquid samples during incubation, each of the liquid samples including an indicator configured to produce a characteristic change in light from the liquid sample when a target microbe metabolizes the indicator during incubation of the liquid sample, the tray including a plurality of compartments configured to partition the liquid samples one from another;

a light sensor for individually sensing light from each of the liquid samples held separately in the plurality of compartments of the tray during incubation of the liquid samples, the light sensor configured to detect a characteristic of the light sensed from each of the separately held liquid samples; and

a controller coupled with the light sensor, the controller configured to analyze the detected characteristic of the light sensed from each of the separately held liquid samples to detect occurrences of the characteristic change in light from respective ones of the separately held liquid samples as the separately held liquid samples are incubated, the controller furnishing an indication that presence of the target microbe is detected while the separately held liquid samples are being incubated when a detected number of occurrences of the characteristic change in light, corresponding to a number of separately held liquid samples, exceeds a threshold number of occurrences of the characteristic change in light from respective ones of a total number of separately held liquid samples, the threshold number of occurrences of the characteristic change in light from respective ones of the total number of separately held liquid samples corresponding to a concentration of the target microbe within the liquid samples that indicates presence of the target microbe,

wherein the concentration of the target microbe within the separately held liquid samples is indicated by the occurrence of the characteristic change in light from a predetermined number of the separately held liquid samples following a predetermined incubation period, and wherein the threshold number of occurrences of the characteristic change in light from respective ones of the total number of separately held liquid samples is less than the predetermined number for an intermediate time period less than the predetermined incubation period, and

wherein the controller furnishes an indication of the absence of the target microbe if the detected number of occurrences of the characteristic change in light from respective ones of the total number of separately held liquid samples does not exceed the threshold number of occurrences of the characteristic change in light from respective ones of the total number of separately held liquid samples within the intermediate time period.

2. The system as recited in claim 1, wherein the liquid samples further include a growth accelerant for accelerating growth of the target microbe.

3. The system as recited in claim 1, wherein the target microbe comprises Escherichia coli (E. coli).

4. The system as recited in claim 1, further comprising a divider for receiving a single liquid sample and equally portioning the single liquid sample into the liquid samples, the plurality of compartments of the tray configured for receiving the liquid samples from the divider.

5. The system as recited in claim 1, further comprising a light source configured to be received in the incubator for illuminating the liquid samples held in the plurality of compartments during incubation of the liquid samples.

6. The system as recited in claim 5, wherein the light source is configured to individually illuminate one of the liquid samples held in the plurality of compartments at a time.

7. A system for detecting the presence of a target microbe comprising:

a tray configured to be received in an incubator for holding liquid samples during incubation, each of the liquid samples being separately held in the tray and including an indicator configured to produce a characteristic change in light from a respective one of the separately held liquid samples when a target microbe metabolizes the indicator;

a light sensor for sensing light from the separately held liquid samples tray during incubation of the liquid samples, the light sensor configured to detect a characteristic of the light sensed; and

a controller coupled with the light sensor, the controller configured to analyze the detected characteristic of the light sensed from the separately held liquid samples to detect occurrences of the characteristic change in light from respective ones of the separately held liquid samples, the controller furnishing an indication that presence of the target microbe is detected while the separately held liquid samples are being incubated when a detected number of occurrences of the characteristic change, corresponding to a number of separately held liquid samples, exceeds a threshold number of occurrences of the characteristic change in light from respective ones of a total number of separately held liquid samples, the threshold number of occurrences of the characteristic change in light from respective ones of the total number of separately held liquid samples corresponding to a concentration of the target microbe within the liquid samples that indicates presence of the target microbe,

wherein the concentration of the target microbe within the separately held liquid samples is indicated by the occurrence of the characteristic change in light from a predetermined number of the separately held liquid samples following a predetermined incubation period, and wherein the threshold number of occurrences of the characteristic change in light from respective ones of the total number of separately held liquid samples is less than the predetermined number for an intermediate time period less than the predetermined incubation period, and

wherein the controller furnishes an indication of the absence of the target microbe if the detected number of occurrences of the characteristic change in light from respective ones of the total number of separately held liquid samples does not exceed the threshold number of occurrences of the characteristic change in light from respective ones of the total number of separately held liquid samples within the intermediate time period.

8. The system as recited in claim 7, wherein the liquid samples further include a growth accelerant for accelerating growth of the target microbe.