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Primers with modified phosphate and base in allele-specific PCR

  • US 9,382,581 B2
  • Filed: 11/27/2013
  • Issued: 07/05/2016
  • Est. Priority Date: 12/13/2012
  • Status: Active Grant
First Claim
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1. A method of allele-specific amplification of a variant of a target sequence, the target existing in the form of several variant sequences, the method comprising:

  • (a) hybridizing a first oligonucleotide and a second oligonucleotide to at least one variant of the target sequence;

    wherein the first oligonucleotide is at least partially complementary to one or more variants of the target sequence, and the second oligonucleotide is at least partially complementary to one or more variants of the target sequence, and has at least one selective nucleotide complementary to only one variant of the target sequence;

    wherein said second oligonucleotide comprises a modified phosphate having a structure;

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