Flow cells for biological or chemical analysis

• US 9,387,476 B2
• Filed: 06/03/2015
• Issued: 07/12/2016
• Est. Priority Date: 10/27/2010
• Status: Expired due to Fees

• Current Assignee: Illumina Incorporated
• Original Assignee: Illumina Incorporated
• Inventors: Eltoukhy, Helmy A., Khurana, Tarun, Javanmardi, Behnam, Sabounchi, Poorya, Aghababazadeh, Majid
• Primary Examiner(s): Sines, Brian J
• Application Number:
  US14/729,809
• Publication Number:
  US 20150266022A1
• Time in Patent Office: 405 Days
• Field of Search: 422/50, 422/68.1, 422/81, 422/502, 422/503, 422/504, 422/509, 422/554, 436/43, 436/63, 436/174, 436/180, 436/177, 436/178
• US Class Current: 1/1
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2200/04 Exchange or ejection of cartridges, containers or reservoirs
• CPC Class Codes: B01L 2200/082 Handling hazardous material
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/022 Transponder chips
• CPC Class Codes: B01L 2300/023 Sending and receiving of information, e.g. using bluetooth
• CPC Class Codes: B01L 2300/048 enabling gas exchange, e.g. vents
• CPC Class Codes: B01L 2300/0627 Sensor or part of a sensor is integrated
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/0819 Microarrays; Biochips
• CPC Class Codes: B01L 2300/0877 Flow chambers
• CPC Class Codes: B01L 2300/161 Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
• CPC Class Codes: B01L 2300/165 Specific details about hydrophobic, oleophobic surfaces
• CPC Class Codes: B01L 2400/0457 passive flow or gravitation
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/0622 distribution valves, valves having multiple inlets and/or outlets, e.g. metering valves, multi-way valves
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/5085 : for multiple samples, e.g. microtitration plates
• CPC Class Codes: B01L 7/52 : with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: C12Q 1/6869 : Methods for sequencing
• CPC Class Codes: C12Q 1/6874 : involving nucleic acid arrays, e.g. sequencing by hybridisation

Abstract

A flow cell including inlet and outlet ports in fluid communication with each other through a flow channel that extends therebetween. The flow channel includes a diffuser region and a field region that is located downstream from the diffuser region. The field region of the flow channel directs fluid along reaction sites where desired reactions occur. The fluid flows through the diffuser region in a first flow direction and through the field region in a second flow direction. The first and second flow directions being substantially perpendicular.

Claims

1. A flow cell comprising inlet and outlet ports in fluid communication with each other through a flow channel that extends therebetween, the flow channel including a diffuser region and a field region that is located downstream from the diffuser region, the flow channel also including a collector region that is located downstream from the field region and configured to receive fluid from the field region, the diffuser region being shaped to increase a cross-sectional area of the flow channel as the diffuser region extends downstream in a first fluid flow direction, the field region of the flow channel directing fluid along reaction sites where desired reactions occur, wherein the fluid flows through the field region in a second flow direction, the first and second flow directions being perpendicular, wherein the fluid is configured to flow through the collector region in a third flow direction that is different than the second flow direction, the collector region being shaped to reduce a cross-sectional area of the flow channel as the collector region extends downstream in the third flow direction.

2. The flow cell in accordance with claim 1 wherein the first flow direction is configured to extend along a gravitational force direction when in operation.

3. The flow cell in accordance with claim 1 wherein a flow cross-section of the field region is defined by dimensions that are uniform throughout the field region.

5. The flow cell in accordance with claim 1 wherein the collector region has a similar shape as the diffuser region.

6. The flow cell in accordance with claim 1 further comprising an engagement area along an exterior surface that is configured to interface with a thermal element, the engagement area extending along the field region of the flow channel.

7. The flow cell in accordance with claim 1 wherein the diffuser region is configured to distribute a uniform flow of fluid through the field region.

8. The flow cell in accordance with claim 1, wherein the flow cell includes a flow cover having the inlet and outlet ports and a substrate layer configured to have the reaction sites, the field region being disposed between the flow cover and the substrate layer.

9. The flow cell in accordance with claim 8, wherein the flow cover and the substrate layer are discrete elements that are secured to each other, the flow cover having a mounting side, the mounting side and the substrate layer forming the field region therebetween when the flow cover is coupled to the substrate layer.

10. The flow cell in accordance with claim 9, wherein the flow cover has an open-sided recess along the mounting side, the diffuser region and the collector region opening into the open-sided recess, the open-sided recess forming the field region when the flow cover is coupled to the substrate layer.

11. The flow cell in accordance with claim 9, wherein the substrate layer includes a plurality of reaction chambers that open to and are in fluid communication with the field region, the reaction chambers being defined by shared walls.

12. The flow cell in accordance with claim 11, wherein the substrate layer includes thru-holes that extend entirely through the substrate layer and form the reaction chambers, the substrate layer configured to be mounted to an intermediate layer or activity detector to enclose the reaction chambers.

4. A flow cell comprising inlet and outlet ports in fluid communication with each other through a flow channel that extends therebetween, the flow channel including a diffuser region and a common field region that is located downstream from the diffuser region, the field region of the flow channel directing fluid along reaction sites where desired reactions occur, wherein the fluid flows through the diffuser region in a first flow direction and through the field region in a second flow direction, the first and second flow directions being perpendicular;

wherein the diffuser region comprises a plurality of inlet channels that provide fluid to the field region, the inlet channels being spaced apart from each other along a dimension of the field region that extends perpendicular to the second fluid direction.

13. The flow cell in accordance with claim 4, wherein the flow cell includes a flow cover having the inlet and outlet ports and a substrate layer configured to have the reaction sites, the field region being disposed between the flow cover and the substrate layer.

14. The flow cell in accordance with claim 13, wherein the flow cover and the substrate layer are discrete elements that are secured to each other, the flow cover having a mounting side, the mounting side and the substrate layer forming the field region therebetween when the flow cover is coupled to the substrate layer.

15. The flow cell in accordance with claim 14, wherein the flow cover has an open-sided recess along the mounting side, the inlet channels opening to the open-sided recess, the open-sided recess forming the field region when the flow cover is coupled to the substrate layer.

16. The flow cell in accordance with claim 14, wherein the substrate layer includes a plurality of reaction chambers that open to and are in fluid communication with the field region, the reaction chambers being defined by shared walls.

17. The flow cell in accordance with claim 16, wherein the substrate layer includes thru-holes that extend entirely through the substrate layer and form the reaction chambers, the substrate layer configured to be mounted to an intermediate layer or activity detector to enclose the reaction chambers.

18. The flow cell in accordance with claim 4, wherein the flow cell includes a platform that has the inlet port and the diffuser region, the platform having a cavity that opens to an exterior of the platform, the cavity being partially filled with a plug to separate the cavity from the exterior and form the diffuser region.

19. The flow cell in accordance with claim 18, wherein the platform is a first platform and the flow cell includes a second platform that includes a collector region, the flow cell having an engagement area along an exterior surface that is configured to interface with a thermal element, the engagement area extending along the field region of the flow channel between the first and second platforms.