Method for counting subject matters to be counted in container

• US 9,388,376 B2
• Filed: 09/11/2013
• Issued: 07/12/2016
• Est. Priority Date: 03/09/2009
• Status: Active Grant

• Current Assignee: TOYO Seikan Kaisha Limited
• Original Assignee: TOYO Seikan Kaisha Limited
• Inventors: Totani, Takahiko, Ota, Kyohei, Suenaga, Ryo, Ishizaki, Yoichi, Tanaka, Satoshi
• Primary Examiner(s): Kim, Taeyoon
• Application Number:
  US14/023,575
• Publication Number:
  US 20140011186A1
• Time in Patent Office: 1,035 Days
• US Class Current: 1/1
• CPC Class Codes: C12M 23/14 Bags
• CPC Class Codes: C12M 23/34 Internal compartments or partitions
• CPC Class Codes: C12M 27/02 Stirrer or mobile mixing elements
• CPC Class Codes: C12M 33/18 Rollers
• CPC Class Codes: C12M 41/36 of biomass, e.g. colony counters or by turbidity measurements electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404
• CPC Class Codes: C12M 41/40 of pressure

Abstract

A method for counting counted targets disposed in a liquid enclosed in a container, includes adjusting a thickness of at least a part of the container; setting at least a part of the adjusted part as a measurement target region; and counting a number of counted targets in the measurement target region.

Claims

1. A method for counting cells disposed in a liquid enclosed in a flexible culture container, comprising:

pressing a part of the container from a top side or a lower side by a pressing plate as a thickness adjusting member, thereby adjusting a thickness of the container;

setting at least a part of the container adjusted by the pressing as a measurement target region; and

counting a number of cells in the measurement target region.

2. The method according to claim 1, further comprising, prior to adjusting the thickness of at least the part of the container, agitating the liquid in the container to equalize the cells in the liquid.

3. The method according to claim 1, further comprising, based on the counted number of the cells, calculating a density of the cells in the liquid, and/or calculating a number of cells throughout the liquid.

4. The method according to claim 1, wherein the counting step of the number of the cells comprises counting the number of the cells in a photographed image of the measurement target region.

5. The method according to claim 1, further comprising reducing the thickness of the container at a portion pressed by pressing the part of the container from the top side or the lower side by the pressing plate when the number of the cells in the measurement target region is equal to or more than a predetermined value, and then counting the number of cells.

6. The method according to claim 1, further comprising increasing the thickness of the container at a portion other than a portion pressed by pressing the part of the container from the top side or the lower side by the pressing plate when the number of the cells in the measurement target region is less than a predetermined value, and then counting the number of cells.

7. The method according to claim 1, wherein at least a part of the pressing plate located above the measurement target region of the culture container is made of a transparent material.