Microbial oils with lowered pour points, dielectric fluids produced therefrom, and related methods

• US 9,388,435 B2
• Filed: 06/04/2015
• Issued: 07/12/2016
• Est. Priority Date: 11/03/2010
• Status: Active Grant

• Current Assignee: Corbion Biotech, Inc. (Corbion NV)
• Original Assignee: Terravia Holdings, Inc. (Corbion NV)
• Inventors: Franklin, Scott, Rakitsky, Walter, Rudenko, George, Zhao, Xinhua, Rodriguez, Felipe Arana, Lu, Wenhua, Wee, Janice
• Primary Examiner(s): MC GINTY, DOUGLAS J
• Application Number:
  US14/730,671
• Publication Number:
  US 20150344917A1
• Time in Patent Office: 404 Days
• Field of Search: 252/578, 336/58, 508/112, 435/137, 435/252.3, 435/257, 435/2, 435/414
• US Class Current: 1/1
• CPC Class Codes: A23D 9/007 characterised by ingredients other than fatty acid triglycerides
• CPC Class Codes: A23D 9/02 characterised by the production or working-up
• CPC Class Codes: C10M 169/041 the additives being macromolecular compounds only
• CPC Class Codes: C11B 1/025 by enzymes or microorganisms, living or dead
• CPC Class Codes: C11B 1/06 by pressing
• CPC Class Codes: C11B 1/10 by extracting
• CPC Class Codes: C11B 3/001 by a combination of two or more of the means hereafter
• CPC Class Codes: C11B 3/006 by extraction
• CPC Class Codes: C11B 3/008 by filtration, e.g. including ultra filtration, dialysis C11B3/005 takes precedence
• CPC Class Codes: C11B 3/06 with bases
• CPC Class Codes: C11B 3/10 by adsorption
• CPC Class Codes: C11B 3/14 with the use of indifferent gases or vapours, e.g. steam
• CPC Class Codes: C12N 15/79 Vectors or expression systems specially adapted for eukaryotic hosts
• CPC Class Codes: C12N 9/16 acting on ester bonds (3.1)
• CPC Class Codes: C12N 9/2442 Chitinase (3.2.1.14)
• CPC Class Codes: C12P 7/64 Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
• CPC Class Codes: C12P 7/6409 Fatty acids
• CPC Class Codes: C12P 7/6463 obtained from glyceride producing microorganisms, e.g. single cell oil
• CPC Class Codes: C12Y 301/02014 Oleoyl-[acyl-carrier-protein] hydrolase (3.1.2.14), i.e. ACP-thioesterase
• CPC Class Codes: H01B 3/20 liquids, e.g. oils silicone oils H01B3/46
• CPC Class Codes: H01F 27/105 : Cooling by special liquid or by liquid of particular composition
• CPC Class Codes: H01F 27/321 : using a fluid for insulating purposes only

Abstract

Methods and compositions for the production of dielectric fluids from lipids produced by microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a lipid pathway modification enzyme, a fatty acyl-ACP thioesterase, a desaturase, a fatty acyl-CoA/aldehyde reductase, and/or an acyl carrier protein are useful in manufacturing dielectric fluids.

Claims

1. A method of producing an RBD microbial oil, wherein the microbial oil has a pour point of less than about −

5°

C., and wherein the fatty acid composition of the microbial oil is at least 50 area % C18;

1 fatty acids and less than 10 area % C18;

2 fatty acids, as determined by fatty acid methyl ester gas chromatography flame ionization detection methods, the method comprising;

a. cultivating a genetically engineered microbe engineered to express one or more exogenous genes until the microbe has at least 10% oil by dry weight;

b. separating the oil from the microbe; and

c. subjecting the oil to refining, bleaching, deodorizing or degumming to produce RBD microbial oil.

2. The method of claim 1, wherein the microbial oil has a pour point of between about −

10°

C. and about −

40°

C.

3. The method of claim 1, wherein the method additionally comprises adding an antioxidant, metal ion deactivator, corrosion inhibitor, demulsifier, anti-wear additive, pour point depressant, or anti-hydrolysis compound to the RBD oil.

4. The method of claim 1, wherein the genetically engineered microbe is Prototheca or Chlorella.

5. The method of claim 4, wherein the genetically engineered microbe is Prototheca moriformis.

6. The method of claim 1, wherein the one or more exogenous genes encode(s) sucrose invertase or fatty acyl-ACP thioesterase.

7. The method of claim 6, wherein the one or more exogenous genes encode(s) two or more fatty acyl-ACP thioesterases.

8. The method of claim 6, wherein the one or more exogenous genes encode(s) sucrose invertase and one or more fatty acyl-ACP thioesterases.

9. A method of producing a product comprising microbial oil, wherein the product has a pour point of less than about −

5°

C., and wherein the fatty acid composition of the microbial oil is at least 50 area % C18;

1 fatty acids and less than 10 area % C18;

2 fatty acids, as determined by fatty acid methyl ester gas chromatography flame ionization detection methods, the method comprising;

a. subjecting a first microbial oil to refining, bleaching, deodorizing or degumming to produce RBD oil, wherein the RBD oil is characterized by an initial pour point and a first temperature;

b. lowering the temperature of the RBD oil to a second temperature, which is lower than the first temperature;

c. holding the RBD oil at the second temperature; and

d. filtering the RBD oil at the second temperature to provide a second microbial oil characterized by a second pour point that is lower than the initial pour point, wherein the second pour point is less than about −

5°

C.

10. The method of claim 9, wherein the microbial oil has a pour point of between about −

10°

C. and about −

40°

C.

11. The method of claim 9, wherein the method additionally comprises adding an antioxidant, metal ion deactivator, corrosion inhibitor, demulsifier, anti-wear additive, pour point depressant, or anti-hydrolysis compound to the second microbial oil.

12. The method of claim 9, wherein the method additionally comprises producing the first microbial oil by:

a. cultivating a genetically engineered microbe engineered to express one or more exogenous genes until the microbe has at least 10% oil by dry weight; and

b. separating the oil from the microbe to produce the first microbial oil.

13. The method of claim 9, wherein the first temperature is between above 15°

C. to about 50°

C., and the second temperature is between about −

15°

C. and about 15°

C.

14. The method of claim 9, wherein the product is a lubricant, a hydraulic fluid, an industrial oil, or a dielectric fluid.

15. The method of claim 14, wherein the product is a dielectric fluid.

16. The method of claim 15, wherein the dielectric fluid further comprises an antioxidant, a metal ion deactivator, a corrosion inhibitor, a demulsifier, an anti-wear additive, a pour point depressant, or an anti-hydrolysis compound.