System and method for processing samples

• US 9,388,448 B2
• Filed: 02/05/2014
• Issued: 07/12/2016
• Est. Priority Date: 12/19/2008
• Status: Active Grant

• Current Assignee: Neogen Food Safety US Holdco Corporation (Neogen Corporation)
• Original Assignee: 3M Innovative Properties Company (3M Company)
• Inventors: Halverson, Kurt J.
• Primary Examiner(s): Reifsnyder, David A
• Application Number:
  US14/173,157
• Publication Number:
  US 20140154731A1
• Time in Patent Office: 888 Days
• US Class Current: 1/1
• CPC Class Codes: C12Q 1/02 involving viable microorganisms
• CPC Class Codes: G01N 1/4077 by other techniques involving separation of suspended solids
• CPC Class Codes: G01N 2001/4088 filtration
• CPC Class Codes: G01N 2035/00148 Test cards, e.g. Biomerieux or McDonnel multiwell test cards
• CPC Class Codes: G01N 35/00029 provided with flat sample substrates, e.g. slides G01N35/028 takes precedence
• CPC Class Codes: Y10T 436/255 including use of a solid sorbent, semipermeable membrane, or liquid extraction
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A system and method for processing samples. The system can include a loading chamber, a detection chamber positioned in fluid communication with the loading chamber, and a fluid path defined at least partially by the loading chamber and the detection chamber. The system can further include a filter positioned such that at least one of its inlet and its outlet is positioned in the fluid path. The method can include positioning a sample in the loading chamber, filtering the sample in the fluid path to form a concentrated sample and a filtrate, removing the filtrate from the fluid path at a location upstream of the detection chamber, moving at least a portion of the concentrated sample in the fluid path to the detection chamber, and analyzing at least a portion of the concentrated sample in the detection chamber for an analyte of interest.

Claims

1. A method for processing samples, the method comprising:

providing a loading chamber;

providing a detection chamber positioned in fluid communication with the loading chamber;

providing a fluid path defined at least partially by the loading chamber and the detection chamber, wherein the fluid path terminates at the detection chamber, such that the detection chamber includes an inlet but no outlet;

positioning a sample in the loading chamber;

filtering the sample with a filter positioned in the loading chamber to form a concentrated sample on the filter and a filtrate, the filter having an inlet and outlet and positioned such that at least one of the inlet and the outlet is positioned in the fluid path, wherein filtering includes applying a pressure differential across the filter;

removing the filtrate from the fluid path at a location upstream of the detection chamber;

moving at least a portion of the concentrated sample from the filter, in the fluid path, to the detection chamber by centrifugation; and

analyzing the at least a portion of the concentrated sample in the detection chamber for an analyte of interest.

2. The method of claim 1, further comprising providing enrichment media in the detection chamber prior to moving at least a portion of the concentrated sample from the filter, in the fluid path, to the detection chamber.

3. The method of claim 1, wherein moving at least a portion of the concentrated sample from the filter, in the fluid path, to the detection chamber by centrifugation includes moving at least a portion of the concentrated sample to the detection chamber without exposing the concentrated sample to ambience.

4. The method of claim 1, wherein the inlet of the filter is positioned in the fluid path and the outlet is positioned such that removing the filtrate from the fluid path includes removing the filtrate via the outlet of the filter and occurs as a result of filtering the sample.

5. The method of claim 1, wherein the inlet and the outlet of the filter are positioned in the fluid path, such that the filtrate is removed from the fluid path at a location that is downstream of the filter.

6. The method of claim 1, wherein the fluid path is a primary fluid path and the filter defines a secondary fluid path, and wherein the secondary fluid path is oriented at an angle with respect to the primary fluid path.

7. The method of claim 1, further comprising eluting the concentrated sample from the filter in the fluid path.

8. The method of claim 1, wherein providing a detection chamber includes providing a plurality of detection chambers positioned in fluid communication with the loading chamber, wherein the fluid path terminates at each detection chamber, and wherein moving at least a portion of the concentrated sample from the filter, in the fluid path, to the detection chamber includes moving at least a portion of the concentrated sample in the fluid path to each of the plurality of detection chambers.

9. The method of claim 1, further comprising inhibiting the concentrated sample in the detection chamber from moving out of the detection chamber.

10. The method of claim 9, wherein inhibiting the concentrated sample in the detection chamber from moving out of the detection chamber includes positioning at least one of an obstruction and a seal in the fluid path upstream of the detection chamber.

11. The method of claim 1, wherein the detection chamber is in fluid communication with the loading chamber via a channel, wherein the fluid path is further defined by the channel, and further comprising sealing the channel after moving at least a portion of the concentrated sample in the fluid path to the detection chamber.

12. The method any claim 1, further comprising sealing at least a portion of the fluid path that is positioned upstream of the detection chamber after moving at least a portion of the concentrated sample from the filter, in the fluid path, to the detection chamber.

13. A system for processing samples, the system comprising:

a loading chamber adapted to receive a sample;

a detection chamber positioned in fluid communication with the loading chamber;

a fluid path defined at least partially by the loading chamber and the detection chamber, wherein the fluid path terminates at the detection chamber, such that the detection chamber includes an inlet but no outlet;

a filter positioned in the loading chamber and having an inlet and an outlet, the filter positioned such that at least one of the inlet and the outlet is positioned in the fluid path, the filter adapted to filter the sample to form a concentrated sample and a filtrate; and

a filtrate outlet positioned such that the filtrate is removed from the fluid path at a location upstream of the detection chamber.

14. The system of claim 13, wherein the fluid path is configured to be sealed upstream of the detection chamber.

15. The system of claim 13, wherein the detection chamber is one of a plurality of detection chambers, wherein the fluid path terminates at each detection chamber, and wherein the fluid path is configured to be sealed upstream of each detection chamber.

16. The system of claim 13, wherein the detection chamber is one of a plurality of detection chambers positioned in fluid communication with the loading chamber, and wherein the loading chamber is one of a plurality of loading chambers, and wherein some of the plurality of detection chambers are positioned in fluid communication with each of the plurality of loading chambers.

17. The system of claim 13, further comprising enrichment media for an analyte of interest that is provided in the detection chamber.

18. The system of claim 17, wherein the enrichment media is present in the detection chamber prior to use.

19. The system of claim 13, further comprising a channel positioned in fluid communication between the loading chamber and the detection chamber, the channel further defining at least a portion of the fluid path.

20. The system of claim 19, wherein the detection chamber is one of a plurality of detection chambers positioned in fluid communication with the loading chamber, and wherein the channel is one of a plurality of channels positioned to fluidly couple the plurality of detection chambers with the loading chamber.

21. The system of claim 19, wherein the detection chamber is one of a plurality of detection chambers positioned in fluid communication with the loading chamber, and wherein the channel is a primary channel, and further comprising a plurality of secondary channels positioned to fluidly couple the plurality of detection chambers with the primary channel.

22. The system of claim 21, wherein the primary channel is configured to be sealed.

23. The system of claim 13, wherein the detection chamber is configured to receive at least a portion of the concentrated sample, and wherein, when at least a portion of the concentrate sample is positioned in the detection chamber, at least a portion of the fluid path upstream of the detection chamber is sealed.