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Modified nucleotides

  • US 9,388,464 B2
  • Filed: 08/07/2015
  • Issued: 07/12/2016
  • Est. Priority Date: 08/23/2002
  • Status: Expired due to Term
First Claim
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1. A method of controlling the incorporation of a nucleotide having a ribose or deoxyribose sugar moiety, and said nucleotide is complementary to a second nucleotide in a target single-stranded polynucleotide in a synthesis or sequencing reaction, comprisingincorporating into the growing complementary polynucleotide said nucleotide, the incorporation of said nucleotide preventing or blocking introduction of subsequent nucleoside or nucleotide molecules into said growing complementary polynucleotide;

  • wherein a protecting group is attached to the 3′

    or the 2′

    oxygen atom of the ribose or deoxyribose sugar moiety of said nucleotide; and

    said nucleotide comprises a detectable label linked by a linker; and

    reacting the nucleotide with a water-soluble phosphine to cleave the linker;

    wherein said detectable label is a fluorophore; and

    wherein said water-soluble phosphine also cleaves said protecting group.

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