Cross-linked enzyme matrix and uses thereof

• US 9,388,503 B2
• Filed: 04/08/2013
• Issued: 07/12/2016
• Est. Priority Date: 05/31/2001
• Status: Expired due to Term

• Current Assignee: Instrumentation Lab Co (Instrumentation Lab)
• Original Assignee: Instrumentation Lab Co (Instrumentation Lab)
• Inventors: Pamidi, Prasad, Mansouri, Sohrab, Shin, Melanie, Cosofret, Vasile, Xu, Clarke
• Primary Examiner(s): Yamasaki, Robert
• Application Number:
  US13/858,501
• Publication Number:
  US 20130220820A1
• Time in Patent Office: 1,191 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: C12Q 1/002 Electrode membranes
• CPC Class Codes: C12Q 1/003 Functionalisation
• CPC Class Codes: C12Q 1/005 involving specific analytes or enzymes including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence
• CPC Class Codes: C25D 9/02 with organic materials
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

An electrochemical sensor system and membrane and method thereof for increased accuracy and effective life of electrochemical and enzyme sensors.

Claims

1. A method of restoring a polymeric membrane in an electrochemical sensor system following repeated exposures to patient samples, comprising:

(i) providing said electrochemical sensor system comprising,

(a) an electrochemical sensor card comprising at least one electrochemical sensor, said at least one electrochemical sensor comprising at least an electrode and a composite membrane deposited on said electrode, said composite membrane comprising an outer layer, an inner polymeric membrane layer adapted to function as an inner interference rejection membrane which protects the electrochemical sensor from fouling or interference from compounds in a sample, and an enzyme layer,

(b) an electrochemical sensor apparatus in electrical contact with the electrochemical sensor card, said electrochemical sensor apparatus being configured to measure electrical signals from the electrochemical sensor card and to provide an electrical potential to the electrochemical sensor, and

(c) a reservoir containing an electropolymerizable monomer in a solution in fluid communication with the electrochemical sensor card;

(ii) contacting the electropolymerizable monomer solution from said reservoir with the composite membrane in the electrochemical sensor via a flow channel; and

,

(iii) applying said electrical potential of sufficient strength and duration from said electrochemical sensor apparatus to cause at least a portion of the electropolymerizable monomer solution to polymerize onto the inner polymeric membrane.

2. The method according to claim 1 wherein the electrical potential is applied at a range of about 0.1 to 0.8 V versus an on-board reference electrode.

3. The method according to claim 1 wherein the electrical potential is applied for a range of time from about 30 seconds to 1 hour.

4. The method according to claim 1 further comprising applying an additional electrical potential of sufficient strength and sufficient duration to said at least an electrode prior to or after said restoration of the inner polymeric membrane to cause removal of at least a portion of an interfering agent in the inner polymeric membrane.

5. The method according to claim 4 wherein the additional electrical potential is in a range of about 0.1 to 0.8 V versus an on-board reference electrode and is applied for a range of time from about 10 to 200 seconds.

6. The method according to claim 1 wherein said electrical potential comprises a range of about 0.1 to 0.8 V versus an on-board reference electrode and is applied for a range of time from about 30 seconds to 1 hour.

7. The method according to claim 1 wherein the electrical potential comprises about 0.5 V versus an on-board reference electrode and is applied for about 3 minutes.

8. The method according to claim 1 wherein said electropolymerizable monomer is selected from the group consisting of benzothiophene, phenylenediamines, and dihydroxybenzenes.

9. The method according to claim 1 wherein said electropolymerizable monomer is a derivative of phenylenediamine.

10. The method according to claim 1 wherein said at least one electrochemical sensor is selected from the group consisting of pH sensor, pCO₂ sensor, pO₂ sensor, Na⁺ sensor, Ca⁺⁺ sensor, glucose sensor, lactate sensor creatine sensor, creatinine sensor and hematocrit sensor and a combination thereof.

11. The method according to claim 1 wherein said electrochemical sensor detects a compound selected from the group consisting of a hydrogen ion, carbon dioxide, oxygen, sodium ion, potassium ion, ionized calcium, chloride, hematocrit, glucose, lactate, creatine, creatinine, urea and a combination thereof.