Separating target analytes using alternating magnetic fields
First Claim
1. A method for separating and analyzing a target analyte in a sample, the method comprising:
- contacting a sample with magnetic particles comprising first moieties specific for a target analyte, wherein a plurality of the particles bind the target analyte in the sample, thereby forming target/particle complexes in the sample, while a plurality of the particles remain unbound in the sample as free particles;
flowing the sample through a channel comprising second moieties attached to at least one surface of the channel, wherein the second moieties are specific to the target analyte;
applying alternating magnetic fields to the flowing sample to result in the target/particle complexes being brought into proximity of the surface to bind the second moieties that are specific to the target analyte while the plurality of unbound particles remain free in the sample;
binding the target/particle complexes to the second moieties so as to form bound target/particle/second moiety complexes;
flowing away unbound particles and unbound non-target components of the sample;
eluting the bound target/particle/second moiety complexes from the surface of the channel; and
analyzing the target/particle/second moiety complexes only after the eluting step, wherein the target/particle/second moiety complexes are not examined in situ on the surface.
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Accused Products
Abstract
The invention generally relates to using magnetic particles and alternating magnet fields to separate a target analyte from a sample. In certain embodiments, methods of the invention involve contacting a sample with magnetic particles including first moieties specific for a target analyte, thereby forming target/particle complexes in the sample, flowing the sample through a channel including second moieties attached to at least one surface of the channel, applying alternating magnetic fields to the flowing sample to result in target/particle complexes being brought into proximity of the surface to bind the second moieties and unbound particles remaining free in the sample, binding the target/particle complexes to the second moieties, and washing away unbound particles and unbound analytes of the sample.
232 Citations
22 Claims
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1. A method for separating and analyzing a target analyte in a sample, the method comprising:
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contacting a sample with magnetic particles comprising first moieties specific for a target analyte, wherein a plurality of the particles bind the target analyte in the sample, thereby forming target/particle complexes in the sample, while a plurality of the particles remain unbound in the sample as free particles; flowing the sample through a channel comprising second moieties attached to at least one surface of the channel, wherein the second moieties are specific to the target analyte; applying alternating magnetic fields to the flowing sample to result in the target/particle complexes being brought into proximity of the surface to bind the second moieties that are specific to the target analyte while the plurality of unbound particles remain free in the sample; binding the target/particle complexes to the second moieties so as to form bound target/particle/second moiety complexes; flowing away unbound particles and unbound non-target components of the sample; eluting the bound target/particle/second moiety complexes from the surface of the channel; and analyzing the target/particle/second moiety complexes only after the eluting step, wherein the target/particle/second moiety complexes are not examined in situ on the surface. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A method for detecting a target analyte in a sample, the method comprising:
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contacting a sample with magnetic particles comprising first moieties specific for a target analyte, wherein a plurality of the particles bind the target analyte in the sample, thereby forming target/particle complexes in the sample, while a plurality of the particles remain unbound in the sample as free particles; flowing the sample through a channel comprising second moieties attached to at least one surface of the channel, wherein the second moieties are specific to the target analyte; applying alternating magnetic fields to the flowing sample to result in target/particle complexes being brought into proximity of the surface of the channel to bind the second moieties that are specific to the target analyte while the plurality of unbound particles remain free in the sample; binding the target/particle complexes to the second moieties so as to form bound target/particle/second moiety complexes; flowing away unbound particles and unbound non-target components of the sample; eluting the target/particle/second moiety complexes from the surface of the channel; and detecting the target/particle/second moiety complexes only after the eluting step, and wherein the target/particle/second moiety complexes are not examined in situ on the surface. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20, 21)
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22. A method for separating and analyzing a target analyte in a sample, the method comprising:
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contacting a sample with magnetic particles comprising first moieties specific for a target analyte, wherein a plurality of the particles bind the target analyte in the sample, thereby forming target/particle complexes in the sample, while a plurality of the particles remain unbound in the sample as free particles; applying a magnetic field to capture target/particle complexes on a surface; washing the target/particle complexes; removing the magnetic field, thereby releasing target/particle complexes from the surface; flowing the sample through a channel comprising second moieties attached to at least one surface of the channel, wherein the second moieties are specific to the target analyte; applying alternating magnetic fields to the flowing sample to result in target/particle complexes being brought into proximity of the surface of the channel to bind the second moieties that are specific to the target analyte while the plurality of unbound particles remain free in the sample; binding the target/particle complexes to the second moieties to form bound target/particle/second moiety complexes; flowing away unbound particles and unbound non-target components of the sample; eluting the bound target/particle/second moiety complexes from the surface of the channel by eluting the second moieties from the surface of the channel; and analyzing the target/particle/second moiety complexes only after the eluting step, wherein the target/particle/second moiety complexes are not examined in situ on the surface.
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Specification