Microscope and method for operating a microscope
First Claim
1. A method for operating a microscope, comprising:
- beaming excitation light to different points of a specimen,point-specifically varying an intensity of the excitation light and measuring point-specifically and quantitatively an intensity of the light reflected by the specimen in at least one spectral range, andautomatically adjusting, by a regulating device, at least one of the intensity or a spectral composition of the excitation light beamed to a specific point of the specimen on the basis of information previously gained from measured data of the specimen concerning at least one of an estimated intensity or an actual intensity of the light reflected in the spectral range by the specific point, such that an integral of the intensity of the light reflected in the spectral range by the specific point during a pixel dwell time is within a predefined value interval,wherein the information concerning the estimated intensity of the light reflected by the point is provided by an initial measurement of the intensity of the light reflected by the point at the start of a pixel exposure time and an adjustment, by the regulation device, to the correct intensity within the pixel exposure time.
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Abstract
The invention relates to a method for operating a microscope in which excitation light is focused on, or beamed to, different points of a specimen, in which an intensity of the excitation light is point-specifically varied and in which an intensity of the light reflected by said specimen in at least one spectral range is measured point-specifically and quantitatively. The method according to the invention is characterized in that the intensity and/or a spectral composition of the excitation light beamed to a specific point of said specimen is automatically adjusted by a regulating device on the basis of information previously gained from measured data of said specimen concerning an estimated or actual intensity of the light reflected in the spectral range by said point such that an integral of the intensity of the light reflected in the spectral range by this point during a pixel dwell time is within a predefined value interval. The invention also relates to a microscope.
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Citations
34 Claims
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1. A method for operating a microscope, comprising:
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beaming excitation light to different points of a specimen, point-specifically varying an intensity of the excitation light and measuring point-specifically and quantitatively an intensity of the light reflected by the specimen in at least one spectral range, and automatically adjusting, by a regulating device, at least one of the intensity or a spectral composition of the excitation light beamed to a specific point of the specimen on the basis of information previously gained from measured data of the specimen concerning at least one of an estimated intensity or an actual intensity of the light reflected in the spectral range by the specific point, such that an integral of the intensity of the light reflected in the spectral range by the specific point during a pixel dwell time is within a predefined value interval, wherein the information concerning the estimated intensity of the light reflected by the point is provided by an initial measurement of the intensity of the light reflected by the point at the start of a pixel exposure time and an adjustment, by the regulation device, to the correct intensity within the pixel exposure time. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 32, 33, 34)
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21. A microscope, comprising:
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a light source for emission of excitation light for microscopic investigation of a specimen, an intensity modulator for varying an intensity of the excitation light, microscope optics for guiding the excitation light to different points of the specimen and for guiding the light reflected by the different points of the specimen to a detector for point-specific and quantitative detection of the intensity of the light reflected by the specimen in at least one spectral range, and a regulating device, which cooperates with the intensity modulator and the detector, for automatically adjusting at least one of the intensity or a spectral composition of the excitation light beamed to a specific point of the specimen on the basis of information, previously gained from measured data of the specimen, concerning an estimated intensity or actual intensity of the light reflected in the spectral range by the specific point, such that an integral of the intensity of the light reflected in the spectral range, as detected by the detector for the specific point, during a pixel dwell time is within a predefined value interval, wherein the information concerning the estimated intensity of the light reflected by the point is provided by an initial measurement of the intensity of the light reflected by the point at the start of a pixel exposure time and an adjustment, by the regulation device, to the correct intensity within the pixel exposure time. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29, 30, 31)
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Specification