Bioagent detection systems, devices, and methods

• US 9,393,564 B2
• Filed: 03/30/2010
• Issued: 07/19/2016
• Est. Priority Date: 03/30/2009
• Status: Active Grant

• Current Assignee: Ibis Biosciences Incorporated
• Original Assignee: Ibis Biosciences Incorporated
• Inventors: Ecker, David J., Hofstadler, Steven A., Sampath, Rangarajan, Blyn, Lawrence B., Hall, Thomas A., Eshoo, Mark W.
• Primary Examiner(s): Crow, Robert T
• Assistant Examiner(s): Dauner, Joseph G
• Application Number:
  US13/259,350
• Publication Number:
  US 20120064523A1
• Time in Patent Office: 2,303 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: B01L 2200/028 Modular arrangements
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2300/027 Digital display, e.g. LCD, LED
• CPC Class Codes: B01L 2400/0415 electrical forces, e.g. electrokinetic
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: B01L 3/52 Containers specially adapted for storing or dispensing a reagent B01L3/02 takes precedence; containers for medical or pharmaceutical purposes A61J1/00; containers in general B65D; storing or dispensing test elements G01N33/4875; automated reagent dispensing G01N35/1002
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: H01J 49/0022 Portable spectrometers, e.g. devices comprising independent power supply, constructional details relating to portability small scale devices per se H01J49/0013 and H01J49/0018

Abstract

The present invention relates to portable systems and devices, and corresponding methods, for detecting bioagents. In particular, the present invention provides systems, devices, and methods that utilize one or more of a sample preparation component, sample analysis component employing broad range primers, and sample detection component.

Claims

1. A portable handheld device comprising:

a handheld detector and a kit cartridge, said handheld detector comprising;

a) a sample preparation chamber configured for isolation of nucleic acids from a sample;

b) a sample analysis chamber configured for analysis of isolated nucleic acids;

c) a sample detection chamber configured for detection of the presence of or identify of a target molecule;

d) a sample input port;

e) a user interface for receiving processing instructions from a user;

f) a display;

g) a processor configured to display an identity of a bioagent on said display; and

h) a removable reagent cartridge input port; and

said kit cartridge comprising;

a removable reagent cartridge comprising two or more broad range primers wherein said broad range primers hybridize to regions of nucleic acid that are conserved between two or more different organisms, and flank a variable region of nucleic acid that differs between said two or more different organisms; and

wherein said sample preparation chamber is in liquid communication with said sample input port and in liquid communication with said removable reagent cartridge in said removable reagent cartridge input port;

wherein said sample analysis chamber is in liquid communication with said sample preparation chamber and in liquid communication with said removable reagent cartridge in said removable reagent cartridge input port; and

wherein said sample detection chamber is in liquid communication with said sample analysis chamber and in liquid communication with said removable reagent cartridge in said removable reagent cartridge input port.

2. The portable handheld device of claim 1, wherein said removable reagent cartridge comprises reagents for nucleic acid amplification.

3. The portable handheld device of claim 1, wherein said removable reagent cartridge comprises reagents for nucleic acid sequencing.

4. The portable handheld device of claim 1, wherein said sample preparation chamber comprises a SCODA gel.

5. The portable handheld device of claim 4, wherein said device is configured to generate an electric field for SCODA separation of nucleic acids.

6. The portable handheld device of claim 4, wherein said SCODA gel comprises immobilized broad range primers.

7. The portable handheld device of claim 6, wherein said immobilized broad range primers amplify target nucleic acid.

8. The portable handheld device of claim 7, wherein at least one of said immobilized broad range primers is a labeled primer.

9. The portable handheld device of claim 7, wherein said identity of said bioagent is displayed without elution of said target nucleic acid from said SCODA gel.

10. The portable handheld device of claim 1, further comprising a database of target nucleic acid sequences or base compositions for identifying an unknown target nucleic acid processed by said device.

11. A portable system comprising the portable handheld device of claim 1.

12. The system of claim 11, wherein said removable reagent cartridge comprises reagents for nucleic acid amplification.

13. The system of claim 11, wherein said removable reagent cartridge comprises reagents for nucleic acid sequencing.

14. The system of claim 11, wherein said sample preparation chamber comprises a SCODA gel.

15. The system of claim 14, wherein said portable handheld device is configured to generate an electric field for SCODA separation of nucleic acids.

16. The system of claim 14, wherein said SCODA gel comprises immobilized broad range primers.

17. The system of claim 16, wherein said immobilized broad range primers amplify target nucleic acid.

18. The system of claim 17, wherein at least one of said immobilized broad range primers is a labeled primer.

19. The system of claim 17, wherein said identity of said bioagent is displayed without elution of said target nucleic acid from said SCODA gel.

20. The system of claim 11, further comprising a database of target nucleic acid sequences or base compositions for identifying an unknown target nucleic acid processed by said system or device.