Neutralization and containment of redox species produced by circumferential electrodes

• US 9,394,167 B2
• Filed: 04/15/2005
• Issued: 07/19/2016
• Est. Priority Date: 04/15/2005
• Status: Active Grant

• Current Assignee: CustomArray, Inc. (Genscript Biotech Corp.)
• Original Assignee: CustomArray, Inc. (Genscript Biotech Corp.)
• Inventors: Leonetti, Joseph, Maurer, Karl, Cooper, John J. Jr., Fujii, H. Sho
• Primary Examiner(s): Wilkins, III, Harry D
• Application Number:
  US11/108,078
• Publication Number:
  US 20060231411A1
• Time in Patent Office: 4,113 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00529 DNA chips
• CPC Class Codes: B01J 2219/00576 fluorophore
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00641 the porous medium being continuous, e.g. porous oxide substrates
• CPC Class Codes: B01J 2219/00653 the compounds being bound to electrodes embedded in or on the solid supports
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00675 In-situ synthesis on the substrate
• CPC Class Codes: B01J 2219/00713 Electrochemical synthesis
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01J 2219/00731 Saccharides
• CPC Class Codes: B01J 2219/00736 Non-biologic macromolecules, e.g. polymeric compounds
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C25B 11/02 characterised by shape or form
• CPC Class Codes: C25B 3/29 Coupling reactions
• CPC Class Codes: C25B 9/17 Cells comprising dimensionally-stable non-movable electrodes; Assemblies of constructional parts thereof

Abstract

There is disclosed an electrode array architecture employing continuous and discontinuous circumferential electrodes. There is further disclosed a process for the neutralization of acid generated at anode(s) by base generated at cathode(s) circumferentially located to each other so as to confine a region of pH change. The cathodes can be displayed as concentric rings (continuous) or as counter electrodes in a cross pattern (discontinuous). In this way reagents, such as acid, generated in a center electrode are countered (neutralized) by reagents, such as base, generated at the corners or at the outer ring.

Claims

1. An electrode array design having:

(a) a semiconductor electrode array having a top surface and a plurality of electrodes, wherein each electrode of the plurality of electrodes is separately addressable, wherein each electrode of the plurality of electrodes has a top conducting surface that makes up a part of the top surface of the semiconductor electrode array, and wherein each electrode of the plurality of electrodes is surrounded by insulating material, wherein each electrode of the plurality of electrodes produces an acid to initiate a synthesis;

(b) a continuous circumferential electrode that surrounds each electrode of the plurality of electrodes to form an outer ring, wherein the continuous circumferential electrode has a top conducting surface and an inner margin and an outer margin, wherein the inner margin borders with the insulating material that surrounds the electrode and the outer margin is surrounded by insulating material, wherein the continuous circumferential electrode produces a base to neutralize the acid formed by each electrode in a region surrounding the continuous circumferential electrode; and

(c) a porous reaction layer of material formed from a mixture of a polysaccharide and a monosaccharide that adsorbs to the top surface and contains free hydroxyl groups or free amine groups or sulfhydryl groups (or combinations thereof) for covalent molecular binding, wherein the porous reaction layer forms a layer over the electrodes.

2. The electrode array of claim 1 wherein the polysaccharide material is a disaccharide.

3. The electrode array of claim 1 wherein the insulating material that surrounds each of the plurality of electrodes has an average distance between each electrode and circumferential electrode of no more than 10 microns from the outer edge of each electrode to the inner edge of the surrounding circumferential electrode.

4. The electrode array of claim 1 wherein the plurality of electrodes are arranged in a pattern of rows and columns and the circumferential electrodes form a continuous grid pattern similar to a tic-tack-toe board.

5. The electrode array of claim 1, where the acid produced by each electrode of the plurality of electrodes reacts with and neutralizes the base produced by the continuous circumferential electrode to contain the base to the region of the continuous circumferential electrode overlying the porous reaction layer.

6. The electrode array of claim 1, where the base produced by the continuous circumferential electrode reacts with and neutralizes the acid produced by the electrode to contain the acid to region of the electrode bounded by the continuous circumferential electrode overlying the porous reaction layer.

7. A synthesis array comprising:

(a) a plurality of electrodes forming a semiconductor electrode array having a top surface, wherein each electrode of the plurality of electrodes is separately addressable, wherein each electrode of the plurality of electrodes has a top conducting surface, wherein each electrode of the plurality of electrodes produces an acid to initiate a synthesis;

(b) a continuous circumferential electrode grid that surrounds each electrode of the plurality of electrodes, wherein the continuous circumferential electrode grid has a top conducting surface, an inner margin and an outer margin, wherein the top conducting surface of the plurality of electrodes and the top conducting surface of the continuous circumferential electrode grid make up the top surface of the semiconductor electrode array, wherein the continuous circumferential electrode produces a base to neutralize the acid formed by each electrode in a region surrounding the continuous circumferential electrode;

(c) a porous reaction layer of material formed from a mixture of a polysaccharide and a monosaccharide that adsorbs to and forms a layer on the top surface and contains one or more species selected from the group consisting of free hydroxyl, free amine, fee sulfhydryl species for covalent molecular binding; and

(d) an insulating material surrounding each electrode of the plurality of electrodes and extending to the inner margin borders of the continuous circumferential electrode, wherein the outer margin of the continuous circumferential electrode is surrounded by the insulating material.

8. The electrode array of claim 7 wherein the continuous circumferential electrode grid is arranged in a series of horizontal and vertical straight lines to form a pattern, wherein two or more of the plurality of electrodes are enclosed by the continuous circumferential electrode grid.

9. The electrode array of claim 8, wherein the continuous circumferential electrode grid outer margin is less than 50 microns distance from the continuous circumferential electrode grid outer margin.

10. The synthesis array of claim 7, where the acid produced by each electrode of the plurality of electrodes reacts with and neutralizes the base produced by the continuous circumferential electrode grid to contain the base to the region of the continuous circumferential electrode grid overlying the porous reaction layer.

11. The synthesis array of claim 7, where the base produced by the continuous circumferential electrode grid reacts with and neutralizes the acid produced by the electrode to contain the acid to region of the electrode bounded by the continuous circumferential electrode grid overlying the porous reaction layer.

12. A synthesis array comprising:

(a) a plurality of electrodes forming a semiconductor electrode array having a top surface, wherein each electrode of the plurality of electrodes is separately addressable, wherein each electrode of the plurality of electrodes has a top conducting surface, wherein each electrode of the plurality of electrodes produces an acid to initiate a synthesis;

(b) a discontinuous circumferential electrode grid that surrounds each electrode of the plurality of electrodes, wherein the discontinuous circumferential electrode grid has a top conducting surface, an inner margin and an outer margin, wherein the top conducting surface of the plurality of electrodes and the top conducting surface of the discontinuous circumferential electrode grid make up the top surface of the semiconductor electrode array, wherein the discontinuous circumferential electrode produces a base to neutralize the acid formed by each electrode in a region surrounding the discontinuous circumferential electrode;

(c) a porous reaction layer of material formed from a mixture of a polysaccharide and a monosaccharide that adsorbs to and forms a layer on the top surface and contains one or more species selected from the group consisting of free hydroxyl, free amine, fee sulfhydryl species for covalent molecular binding; and

(d) an insulating material surrounding each electrode of the plurality of electrodes and extending to the inner margin borders of the discontinuous circumferential electrode, wherein the outer margin of the discontinuous circumferential electrode is surrounded by the insulating material.

13. The electrode array of claim 12 wherein at least four neighboring electrodes form the discontinuous circumferential electrode grid.

14. The electrode array of claim 12 wherein the discontinuous circumferential electrode grid is arranged in a pattern selected from the group consisting of nearest neighbor, neighbor electrodes, border of neighbor electrodes, border of nearest-neighbor, one closest available electrode, and electrode that is not approach, or a combination pattern thereof.

15. A synthesis array comprising:

(a) a plurality of electrodes forming a semiconductor electrode array having a top surface, wherein each electrode of the plurality of electrodes is separately addressable, wherein each electrode of the plurality of electrodes has a top conducting surface, wherein each electrode of the plurality of electrodes produces an acid to initiate a synthesis;

(b) a continuous circumferential electrode grid that surrounds each electrode of the plurality of electrodes, wherein the continuous circumferential electrode grid has a top conducting surface, an inner margin and an outer margin, wherein the top conducting surface of the plurality of electrodes and the top conducting surface of the continuous circumferential electrode grid make up the top surface of the semiconductor electrode array, wherein the continuous circumferential electrode produces a base to neutralize the acid formed by each electrode in a region surrounding the continuous circumferential electrode;

(c) a porous reaction layer of material formed from a mixture of a polysaccharide and a monosaccharide that adsorbs to and forms a layer on the top surface and contains one or more species selected from the group consisting of free hydroxyl, free amine, fee sulfhydryl species for covalent molecular binding;

(d) an insulating material surrounding each electrode of the plurality of electrodes and extending to the inner margin borders of the continuous circumferential electrode, wherein the outer margin of the continuous circumferential electrode is surrounded by the insulating material;

wherein one or more electrodes from the plurality of electrodes can function as an anode in a first synthesis and one or more electrodes from the plurality of electrodes can function as an anode in a second synthesis.

16. The synthesis array of claim 15, where the continuous circumferential electrode grid is arranged in a series of horizontal and vertical straight lines to form a pattern, where two or more of the plurality of electrodes are enclosed by the continuous circumferential electrode grid.

17. The synthesis array of claim 15, where the continuous circumferential electrode grid outer margin is less than 50 microns distance from the continuous circumferential electrode grid outer margin.