Compositions for linking DNA-binding domains and cleavage domains
First Claim
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1. A fusion protein comprising from N-terminus to C-terminus:
- a DNA-binding domain that binds to a nucleotide target site;
a ZC linker (SEQ ID NO;
2); and
a FokI cleavage domain, wherein the FokI cleavage domain comprises the sequence shown in residues 158 to 356 of SEQ ID NO;
3 or residues 158 to 360 of SEQ ID NO;
4.
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Abstract
Disclosed herein are compositions for linking DNA binding domains and cleavage domains (or cleavage half-domains) to form non-naturally occurring nucleases. Also described are methods of making and using compositions comprising these linkers.
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Citations
14 Claims
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1. A fusion protein comprising from N-terminus to C-terminus:
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a DNA-binding domain that binds to a nucleotide target site; a ZC linker (SEQ ID NO;
2); anda FokI cleavage domain, wherein the FokI cleavage domain comprises the sequence shown in residues 158 to 356 of SEQ ID NO;
3 or residues 158 to 360 of SEQ ID NO;
4. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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Specification