Fluid transport in microfluidic applications with sensors for detecting fluid presence and pressure

• US 9,399,216 B2
• Filed: 12/30/2013
• Issued: 07/26/2016
• Est. Priority Date: 12/30/2013
• Status: Active Grant

• Current Assignee: General Electric Company
• Original Assignee: General Electric Company
• Inventors: Salvamoser, Ruben Julian, Samper, Victor Donald, Rensch, Christian Friedrich Peter, Boeld, Christoph
• Primary Examiner(s): Soderquist, Arlen
• Application Number:
  US14/144,066
• Publication Number:
  US 20150182964A1
• Time in Patent Office: 939 Days
• Field of Search: 422/67, 422/502, 422/504, 422/509, 436/180
• US Class Current: 1/1
• CPC Class Codes: B01J 19/004 Multifunctional apparatus for automatic manufacturing of various chemical products sequential reactions B01J19/0046
• CPC Class Codes: B01J 19/0093 Microreactors, e.g. miniaturised or microfabricated reactors laboratory containers with capillary fluid transport in microfabricated channels or chambers B01L3/5027
• CPC Class Codes: B01J 2219/00783 Laminate assemblies, i.e. the reactor comprising a stack of plates
• CPC Class Codes: B01J 2219/0084 For changing surface tension
• CPC Class Codes: B01J 2219/00891 Feeding or evacuation
• CPC Class Codes: B01J 2219/00903 Segmented flow
• CPC Class Codes: B01J 2219/00959 Flow
• CPC Class Codes: B01J 2219/00963 Pressure
• CPC Class Codes: B01J 2219/0097 Optical sensors
• CPC Class Codes: B01J 2219/00986 Microprocessor
• CPC Class Codes: B01L 2400/08 Regulating or influencing the flow resistance
• CPC Class Codes: B01L 3/0293 for liquids
• CPC Class Codes: B01L 3/502746 characterised by the means for controlling flow resistance, e.g. flow controllers, baffles B01L3/502738 takes precedence
• CPC Class Codes: G01F 22/02 involving measurement of pressure
• CPC Class Codes: Y10T 137/0318 Processes

Abstract

A method includes flowing a liquid into a channel of a microfluidic cassette filled with a gas. The channel includes an inlet section and an outlet section. The method also includes detecting the liquid, the gas, or a combination thereof at a measuring location within the inlet section. The measuring location has a first sensor that may detect a signal indicative of a presence of the liquid, the gas, or a combination thereof. The method also includes compressing the gas, determining a pressure change of the gas within the channel, and determining a volume of the liquid within the channel based on the pressure change of the gas.

Claims

1. A method comprising:

flowing a liquid into a channel of a microfluidic cassette filled with a gas, wherein the channel comprises an inlet section and an outlet section;

detecting the liquid, the gas, or a combination thereof at a measuring location within the inlet section, wherein the measuring location has a first sensor configured to detect a signal indicative of a presence of the liquid, the gas, or a combination thereof;

compressing the gas;

determining a pressure change of the gas within the channel; and

determining a volume of the liquid within the channel based on the pressure change of the gas.

2. The method of claim 1, comprising closing a valve on the outlet section in response to the detection of the liquid.

3. The method of claim 1, comprising filling a portion of the channel with the liquid to compress the gas.

4. The method of claim 1, wherein the outlet section comprises a second sensor configured to measure the pressure of the gas within the channel.

5. The method of claim 1, wherein compression of the gas creates a pressure differential across the liquid.

6. The method of claim 1, comprising delivering the volume of the liquid to a compartment within the microfluidic chip, wherein a pressure differential across the reagent moves the liquid into the compartment.

7. The method of claim 1, comprising filtering the gas, wherein the gas is filtered by one or more filters disposed between a gas source and the microfluidic cassette.

8. The method of claim 1, wherein the signal is representative of a refractive index of the liquid, the gas, or a combination thereof at the measuring location.

9. The method of claim 1, wherein the channel is configured to dissipate heat.

10. The method of claim 1, wherein the channel comprises a known volume.

11. A system comprising:

a microfluidic chip comprising;

a first compartment configured to store a reagent and is fluidly coupled to a second compartment via a first channel;

a metering chamber disposed along the first channel, wherein the metering chamber comprises a second channel having an inlet section and an outlet section, and wherein the metering chamber is configured to meter a volume of the reagent to the second compartment based on a pressure change of a gas within the second channel; and

a control system communicatively coupled to the microfluidic chip, wherein the control system is configured to provide instructions to one or more valves associated with the microfluidic chip, wherein the one or more valves are configured to control the influx and efflux of the reagent, the gas, or a combination thereof within the metering chamber.

12. The system of claim 11, wherein the metering chamber comprises a sensor disposed at a measuring site within the inlet section and configured to detect a fluid, wherein the fluid comprises the reagent, a gas, or a combination thereof.

13. The system of claim 11, wherein the outlet section comprises a sensor configured to detect a pressure of the gas within the second channel.

14. The system of claim 11, wherein the one or more valves are disposed at the outlet section and are configured to close upon detection of the reagent to contain the gas within the second channel.

15. The system of claim 11, wherein a flow of the reagent compresses the gas within the second channel to form a compressed gas.

16. The system of claim 11, wherein a compressed gas creates a pressure differential across the reagent, and wherein the pressure differential causes the reagent to flow into the second chamber.

17. The system of claim 11, wherein the second channel is configured to dissipate heat at a rate that is faster than a flow rate of the reagent into the second channel.

18. A microfluidic chip comprising:

a first chamber configured to store a reagent and fluidly coupled to a second chamber via a first channel;

a metering chamber disposed along the first channel, wherein the metering chamber comprises a second channel having an inlet section and an outlet section, and wherein the outlet section is configured to be coupled to a gas source;

a first sensor disposed at a measuring site within the inlet section, wherein the first sensor is configured to detect a fluid within the measuring site; and

a second sensor disposed at the outlet section, wherein the second sensor is configured to detect a pressure of the fluid.

19. The microfluidic chip of claim 18 comprising, a first valve disposed at the inlet section and a second valve disposed at the outlet section, wherein the first and second valves are configured to control an influx and efflux of the fluid from the second channel.

20. The microfluidic chip of claim 18, wherein the second channel has a defined geometry and a known volume.