Devices and systems for isolating biomolecules and associated methods thereof

• US 9,399,986 B2
• Filed: 08/30/2012
• Issued: 07/26/2016
• Est. Priority Date: 07/31/2012
• Status: Active Grant

• Current Assignee: Global Life Sciences Solutions Operations UK Ltd. (Danaher Corporation)
• Original Assignee: General Electric Company
• Inventors: Nelson, John Richard, Zhu, Li, Chen, Xiaohui, Puleo, Christopher Michael, Spooner, Patrick McCoy
• Primary Examiner(s): Beisner, William H
• Assistant Examiner(s): HENKEL, DANIELLE B
• Application Number:
  US13/599,131
• Publication Number:
  US 20140039172A1
• Time in Patent Office: 1,426 Days
• Field of Search: 435/287.3, 536/23.1, 536/25.24, 210/416.1, 210/143
• US Class Current: 1/1
• CPC Class Codes: F04B 19/006 Micropumps F04B43/043 and F04B43/095 take precedence
• CPC Class Codes: F04B 19/04 Pumps for special use
• CPC Class Codes: F04B 19/20 Other positive-displacement pumps

Abstract

A device, a system, and a method for isolating biomolecules from biological materials are provided. The device comprises a quartz-based solid phase extraction matrix comprising one or more reagents impregnated therein; and an electroosmotic pump (EOP) operationally coupled to the quartz-based solid phase extraction matrix to elute the nucleic acids, wherein the EOP comprises a plurality of electroosmotic membranes comprising one or more positive electroosmotic membranes and one or more negative electroosmotic membranes disposed alternatively and a plurality of electrodes comprising one or more cathodes and one or more anodes, wherein at least one cathode is disposed on one side of one of the membranes and at least one anode is disposed on another side of that membrane and at least one cathode or anode is disposed between a positive electroosmotic membrane and a negative electroosmotic membrane.

Claims

1. A device for isolating nucleic acids, comprising:

a quartz-based solid phase extraction matrix; and

an electroosmotic pump (EOP) operationally coupled to the quartz-based solid phase extraction matrix to elute the nucleic acids,

wherein the EOP comprises a plurality of electroosmotic membranes comprising one or more positive electroosmotic membranes and one or more negative electroosmotic membranes disposed alternatively and a plurality of electrodes comprising one or more cathodes and one or more anodes, wherein at least one cathode is disposed on one side of one of the electroosmotic membranes and at least one anode is disposed on another side of that electroosmotic membrane and at least one cathode or anode is disposed between a positive electroosmotic membrane and a negative electroosmotic membrane, and

wherein the EOP generates a pressure of greater than or equal to 1 PSI by application of a voltage of less than or equal to 3 volts.

2. The device of claim 1, wherein the solid phase extraction matrix comprises one or more reagents impregnated therein.

3. The device of claim 2, wherein one or more of the reagents comprise a cell lysis reagent, a nucleic acid stabilizing reagent, buffer reagents, or combinations thereof.

4. The device of claim 1, further comprising one or more valves.

5. The device of claim 1, wherein the nucleic acids are isolated from a biological material comprising a blood, plasma, serum, buccal swabs, sputum, spores, bacteria, plant, tissue sample, cell sample, cellular extract, and combinations thereof.

6. The device of claim 1, wherein the nucleic acids comprise deoxyribo nucleic acids (DNAs) or ribo nucleic acids (RNA).

7. The device of claim 1, wherein the EOP is configured to elute the nucleic acids in a substantially intact form.

8. The device of claim 1, wherein the EOP is configured to elute the nucleic acids of greater than or equals to 20 kb.

9. The device of claim 1, further comprises a reagent storage location comprising dried buffer reagents or reagents for nucleic acid extraction.

10. The device of claim 1, wherein the EOP is a self contained pump comprising rechargable electrodes.

11. The device of claim 1 is fully automated or partially automated.

12. The device of claim 1 is configured to integrate with an external device or a system.

13. A system, comprising:

a device for isolating nucleic acids, comprising;

a quartz based extraction matrix; and

an electroosmotic pump (EOP) operationally coupled to the quartz based extraction matrix, wherein the EOP comprises a plurality of electroosmotic membranes comprising one or more positive electroosmotic membranes and one or more negative electroosmotic membranes disposed alternatively and a plurality of electrodes comprising one or more cathodes and one or more anodes, wherein at least one cathode is disposed on one side of one of the electroosmotic membranes and at least one anode is disposed on another side of that electroosmotic membrane and at least one cathode or anode is disposed between a positive electroosmotic membrane and a negative electroosmotic membrane, wherein the EOP generates a pressure of greater than or equal to 1 PSI by an application of a voltage of less than or equal to 3 volts;

one or more reservoirs comprising a buffer, a solvent, a reagent or combinations thereof,

a fluidic circuit for flowing liquid through the device, and

a controller.