Methine-substituted cyanine dye compounds
First Claim
1. A complex comprising a nucleic acid reporter compound non-covalently associated with a nucleic acid molecule, wherein the nucleic acid reporter compound has the formula:
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Abstract
Cyanine dye compounds having a substituted methine moiety that are nucleic acid stains, particularly for fluorescent staining of RNA, including compounds having the formula
where R1 is a C1-C6 alkyl, sulfoalkyl, carboxyalkyl or C1-C6 alkoxy; each R2 is independently selected from the group consisting of H, C1-C6 alkyl, C1-C6 alkoxy, fused benzo, trifluoromethyl, amino, sulfo, carboxy and halogen, that is optionally further substituted; at least one of R3, R4, and R5 is an alkyl, aryl, heteroaryl, cyclic, or heterocyclic moiety that is optionally substituted by alkyl, amino, aminoalkyl, carboxy, nitro, or halogen; and the remaining R3, R4 or R5 are hydrogen; X is S, O, or Se; and D is a substituted or unsubstituted pyridinium, quinolinium or benzazolium moiety.
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Citations
20 Claims
- 1. A complex comprising a nucleic acid reporter compound non-covalently associated with a nucleic acid molecule, wherein the nucleic acid reporter compound has the formula:
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4. A method for detecting the presence or absence of nucleic acid in a sample, wherein the method comprises:
a) combining a nucleic acid reporter compound with the sample to prepare a labeling mixture, wherein the nucleic acid reporter compound has the formula; - View Dependent Claims (5, 6, 7, 8, 9)
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11. A method for detecting RNA in the presence of DNA, wherein the method comprises the steps:
a) combining a nucleic acid reporter compound with a sample to prepare a labeling mixture, wherein the nucleic acid reporter compound has a RNA/DNA ratio of fluorescence enhancement greater than about one and wherein the nucleic acid reporter compound has the formula - View Dependent Claims (12, 13, 14, 15, 16, 17)
Specification