Compositions and methods for nucleic acid sequencing
CAFCFirst Claim
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1. A method of sequencing nucleic acid molecules with improved accuracy, comprising:
- providing said nucleic acid molecules, each of said nucleic acid molecules comprising a double-stranded portion, the double-stranded portion consisting of a forward strand and a reverse strand, wherein the forward strand and the reverse strand are fully complementary to each other;
linking a 3′
end of the forward strand to a 5′
end of the reverse strand of each of said nucleic acid molecules, thereby forming a plurality of template molecules, wherein said 3′
end of the forward strand and said 5′
end of the reverse strand are linked by a linking oligonucleotide;
performing at least one single-molecule sequencing process on each of said plurality of template molecules, wherein the forward strand and the reverse strand in each of said plurality of template molecules are fully complementary to each other, each of said at least one single-molecule sequencing process consisting of sequencing only one single template molecule of said plurality of template molecules, thereby generating a sequence read from each of said plurality of template molecules, said sequence read comprising sequences of both the forward strand and the reverse strand of each of said plurality of template molecules; and
determining the sequence of each of said plurality of template molecules by analyzing said sequence read from each of said plurality of template molecules, including comparing the sequences of the forward strand and the reverse strand from each of said plurality of template molecules, thereby improving sequencing accuracy of said nucleic acid molecules.
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Abstract
Compositions and methods for nucleic acid sequencing include template constructs that comprise double stranded portions in a partially or completely contiguous constructs, to provide for redundant sequence determination through one or both of sequencing sense and antisense strands, and iteratively sequencing the entire construct multiple times. Additional sequence components are also optionally included within such template constructs. Methods are also provided for the use and preparation of these constructs as well as sequencing compositions for their application.
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Citations
27 Claims
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1. A method of sequencing nucleic acid molecules with improved accuracy, comprising:
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providing said nucleic acid molecules, each of said nucleic acid molecules comprising a double-stranded portion, the double-stranded portion consisting of a forward strand and a reverse strand, wherein the forward strand and the reverse strand are fully complementary to each other; linking a 3′
end of the forward strand to a 5′
end of the reverse strand of each of said nucleic acid molecules, thereby forming a plurality of template molecules, wherein said 3′
end of the forward strand and said 5′
end of the reverse strand are linked by a linking oligonucleotide;performing at least one single-molecule sequencing process on each of said plurality of template molecules, wherein the forward strand and the reverse strand in each of said plurality of template molecules are fully complementary to each other, each of said at least one single-molecule sequencing process consisting of sequencing only one single template molecule of said plurality of template molecules, thereby generating a sequence read from each of said plurality of template molecules, said sequence read comprising sequences of both the forward strand and the reverse strand of each of said plurality of template molecules; and determining the sequence of each of said plurality of template molecules by analyzing said sequence read from each of said plurality of template molecules, including comparing the sequences of the forward strand and the reverse strand from each of said plurality of template molecules, thereby improving sequencing accuracy of said nucleic acid molecules. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A method of generating nucleotide sequence data for nucleic acid sequencing template molecules, comprising:
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providing said nucleic acid sequencing template molecules, each of said nucleic acid sequencing template molecules consisting of;
two nucleic acid strands that are fully complementary to each other and are linked at their one end by a connecting nucleic acid that links a 3′
end of one strand of the two nucleic acid strands to a 5′
end of another strand of the two nucleic acid strands;performing at least one single-molecule sequencing process on each of said nucleic acid sequencing template molecules, each of said at least one single-molecule sequencing process consisting of sequencing only one single template molecule of said nucleic acid sequencing template molecules; and monitoring said at least one single molecule sequencing process, thereby generating nucleotide sequence data for each of said two nucleic acid strands of each of said nucleic acid sequencing template molecules, and comparing the sequence data of the two nucleic acid strands from the same nucleic acid template molecule of said nucleic acid sequencing template molecules. - View Dependent Claims (15, 16, 17, 18, 19)
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20. A method of sequencing a linear, single nucleic acid template with improved accuracy, comprising:
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providing a nucleic acid molecule comprising a double-stranded nucleic acid segment consisting of a first strand and a second strand, wherein the first strand and the second strand are fully complementary to each other; ligating a linking oligonucleotide to a 3′
end of said first strand and a 5′
end of said second strand, wherein the linking oligonucleotide connects said first strand to said second strand of said double-stranded nucleic acid segment, thereby forming a linear, single-stranded nucleic acid sequencing template molecule comprising both said first strand and said second strand, wherein the first strand and the second strand of said linear, single-stranded nucleic acid sequencing template molecule are fully complementary to each other;performing single-molecule sequencing of said linear, single-stranded nucleic acid sequencing template molecule, said single-molecule sequencing consisting of sequencing only one single template molecule of said linear, single-stranded nucleic acid sequencing template molecule, thereby obtaining a sequence read for said linear, single-stranded nucleic acid sequencing template molecule, wherein said sequence read comprises sequences of said first strand and said second strand of said double-stranded nucleic acid segment, and determining a sequence of the nucleic acid segment by analyzing said sequencing read, including comparing the sequences of said first strand and said second strand from said linear, single-stranded nucleic acid sequencing template molecule, thereby improving sequencing accuracy of said linear, single-stranded nucleic acid sequencing template molecule. - View Dependent Claims (21, 22, 23, 24, 25, 26, 27)
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Specification