Feline probiotic bifidobacteria and methods

• US 9,404,162 B2
• Filed: 04/18/2008
• Issued: 08/02/2016
• Est. Priority Date: 05/31/2005
• Status: Active Grant

• Current Assignee: Mars Incorporated
• Original Assignee: Mars Incorporated
• Inventors: Boileau, Thomas William-Maxwell, Kiely, Barry Pius, O'Mahony, Liam Diarmuid, MacSharry, John, Sunvold, Greg Dean
• Primary Examiner(s): Ware, Debbie K
• Application Number:
  US12/148,461
• Publication Number:
  US 20090214499A1
• Time in Patent Office: 3,028 Days
• US Class Current: 1/1
• CPC Class Codes: A23C 19/0323 using only lactic acid bacteria, e.g. Pediococcus and Leuconostoc species; Bifidobacteria; Microbial starters in general using moulds A23C19/0682
• CPC Class Codes: A23C 9/1234 characterised by using a Lactobacillus sp. other than Lactobacillus Bulgaricus, including Bificlobacterium sp.
• CPC Class Codes: A23K 10/18 of live microorganisms
• CPC Class Codes: A23K 50/40 for carnivorous animals, e.g. cats or dogs
• CPC Class Codes: A23K 50/42 Dry feed
• CPC Class Codes: A23K 50/48 Moist feed
• CPC Class Codes: A61K 35/745 Bifidobacteria
• CPC Class Codes: A61P 1/12 Antidiarrhoeals
• CPC Class Codes: A61P 1/14 Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
• CPC Class Codes: A61P 17/00 Drugs for dermatological disorders
• CPC Class Codes: A61P 31/00 Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C12N 1/20 Bacteria; Culture media therefor
• CPC Class Codes: C12N 1/205 Bacterial isolates
• CPC Class Codes: C12R 2001/01 Bacteria or Actinomycetales ; using bacteria or Actinomycetales
• CPC Class Codes: Y10S 435/822 using bacteria or actinomycetales

Abstract

According to the invention there is provided a strain of lactic acid bacteria of the genus Bifidobacteria obtainable by isolation from resected and washed feline gastrointestinal tract having a probiotic activity in animals. Methods of use and compositions comprising the Bifidobacteria of the present invention are also provided.

Claims

1. A method of treating diarrhea in a feline comprising orally administering to the feline a composition comprising Bifidobacterium longum in an amount effective to treat diarrhea in the feline, wherein the bacteria comprises probiotic activity and the 16s-23s intergenic polynucleotide sequence having greater than 95% homology to the polynucleotide sequence of SEQ ID NO:

1 or greater than 94% homology to the polynucleotide sequence of SEQ ID NO;

2, or a mixture thereof, wherein the bacteria was obtained from bacteria isolated from resected and washed feline gastrointestinal tract.

2. The method of claim 1 wherein the orally administering occurs as part of a normal dietary intake.

3. The method of claim 1 wherein the orally administering occurs as part of a supplement.

4. The method of claim 1 wherein the orally administering occurs at least once per month.

5. The method of claim 1 wherein the amount of bacteria in the composition is from about 1E+04 CFU to about 1E+14 CFU.

6. The method of claim 1 wherein the bacteria in the composition is in viable form or in the form of killed cells.

7. The method of claim 1, wherein the bacteria in the composition is selected from the group consisting of Bifidobacterium longum strain NCIMB 41290, Bifidobacterium longum strain NCIMB 41291, and a mixture of Bifidobacterium longum strains NCIMB 41290 and NCIMB 41291.

8. A method of preventing diarrhea in a feline comprising orally administering to the feline a composition comprising Bifidobacterium longum in an amount effective to treat diarrhea in the feline, wherein the bacteria comprises probiotic activity and the 16s-23s intergenic polynucleotide sequence having greater than 95% homology to the polynucleotide sequence of SEQ ID NO. 1 or greater than 94% homology to the polynucleotide sequence of SEQ ID NO:

2, or a mixture thereof, wherein the bacteria was obtained from bacteria isolated from resected and washed feline gastrointestinal tract.

9. The method of claim 8 wherein the orally administering occurs as part of a normal dietary intake.

10. The method of claim 8 wherein the orally administering occurs as part of a supplement.

11. The method of claim 8 wherein the orally administering occurs at least once per month.

12. The method of claim 8 wherein the amount of bacteria in the composition is from about 1E+04 CFU to about 1E+14 CFU.

13. The method of claim 8 wherein the bacteria in the composition is in viable from or in the form of killed cells.

14. The method of claim 8, wherein the bacteria in the composition is selected from the group consisting of Bifidobacterium longum strain NCIMB 41290, Bifidobacterium longum strain NCIMB 41291, and a mixture of Bifidobacterium longum strains NCIMB 41290 and NCIMB 41291.