Measuring device, measuring system, measuring method, control program, and recording medium

• US 9,404,868 B2
• Filed: 09/07/2011
• Issued: 08/02/2016
• Est. Priority Date: 09/09/2010
• Status: Active Grant

• Current Assignee: Sharp Life Science Corporation
• Original Assignee: Sharp Kabushiki Kaisha (Hon Hai Precision Industry Co., Ltd.)
• Inventors: Yamanaka, Mikihiro, Hijikuro, Megumi, Hara, Keita
• Primary Examiner(s): Porta, David
• Assistant Examiner(s): Malevic, Djura
• Application Number:
  US13/821,464
• Publication Number:
  US 20130168573A1
• Time in Patent Office: 1,791 Days
• Field of Search: 250/365, 250/372, 250/461.1
• US Class Current: 1/1
• CPC Class Codes: A61B 5/0062 Arrangements for scanning
• CPC Class Codes: A61B 5/0064 Body surface scanning
• CPC Class Codes: A61B 5/0071 by measuring fluorescence emission
• CPC Class Codes: A61B 5/14546 for measuring analytes not otherwise provided for, e.g. ions, cytochromes
• CPC Class Codes: A61B 5/489 Blood vessels
• CPC Class Codes: G01N 2021/6417 Spectrofluorimetric devices
• CPC Class Codes: G01N 21/6456 Spatial resolved fluorescence measurements; Imaging
• CPC Class Codes: G01N 21/6486 Measuring fluorescence of biological material, e.g. DNA, RNA, cells G01N21/6428 takes precedence

Abstract

A measuring device (9) of the present invention includes a detector control module (31) for obtaining, from a scanning mechanism including an excitation light source (4) for emitting excitation light and a detector (8) for receiving fluorescence generated from the measurement target upon irradiation with the excitation light, measurement data of the fluorescence, a position information obtaining module (32) for obtaining, from a driving mechanism for controlling relative positions of the scanning mechanism and the measurement target, information of a position where the measurement target is irradiated with the excitation light when the measurement data is obtained, and a fluorescence characteristic management module (33) for preparing fluorescence characteristic data including the measurement data and the position information.

Claims

1. A measuring device, comprising:

measurement data obtaining means for obtaining, from a scanning mechanism including an excitation light irradiation unit for irradiating a measurement target with excitation light and a light receiving unit for receiving fluorescence generated from the measurement target upon irradiation with the excitation light, measurement data representing optical characteristics of the fluorescence;

position information obtaining means for obtaining, from a driving mechanism for controlling relative positions of the scanning mechanism and the measurement target, position information indicating an irradiation position where the measurement target is irradiated with the excitation light when the measurement data is obtained;

fluorescence characteristic management means for extracting, as a feature variable, fluorescence intensity at a particular florescence wavelength from the measurement data, and for preparing fluorescence characteristic data including the position information and the feature variable;

fluorescence characteristic analysis means for preparing map information by plotting the feature variable on a map based on the position information; and

image preparation means for preparing a visualization image to display the map information on a display unit,

wherein the excitation light irradiation unit includes plural types of light sources having different excitation wavelengths,

the measurement data obtaining means obtains measurement data that differs per the excitation wavelength,

the fluorescence characteristic management means prepares the fluorescence characteristic data per the position information and per the excitation wavelength,

the fluorescence characteristic analysis means compares, per the position information, fluorescence intensity in the measurement data linked in correspondence relation with a first excitation wavelength and fluorescence intensity in the measurement data linked in correspondence relation with a second excitation wavelength, and assigns information indicating that a result of the comparison satisfies a predetermined condition to the feature variable plotted at a position indicated by the position information, and

the image preparation means prepares the visualization image in a way of highlighting the feature variable to which the information is assigned.

2. The measuring device according to claim 1, wherein the fluorescence characteristic management means prepares, each time the relative positions are changed, the fluorescence characteristic data per the position information corresponding to the relevant relative positions.

3. The measuring device according to claim 1, wherein the position information included in the fluorescence characteristic data is one-dimensional information for specifying a Y-coordinate of the irradiation position in the measurement target.

4. The measuring device according to claim 1, wherein the position information included in the fluorescence characteristic data is two-dimensional information for specifying X- and Y-coordinates of the irradiation position in the measurement target.

5. The measuring device according to claim 1, wherein the first excitation wavelength and the second excitation wavelength have respective wavelength ranges adapted for measuring Advanced Glycation Endproducts.

6. The measuring device according to claim 1, wherein the first excitation wavelength is 365 nm and the second excitation wavelength is 405 nm.

7. The measuring device according to claim 1,

wherein the fluorescence characteristic management means extracts, a first feature variable representing intensity at a first fluorescence wavelength and a second feature variable representing fluorescence intensity at a second-florescence wavelength;

the image preparation means prepares the visualization image by superimposing the map information based on the first feature variable and the map information based on the second feature variable.

8. The measuring device according to claim 7, wherein an excitation wavelength of the excitation light irradiation unit, the first fluorescence wavelength, and the second fluorescence wavelength have respective wavelength ranges adapted for measuring Advanced Glycation Endproducts existing in blood vessels and specifying positions thereof.

9. The measuring device according to claim 7, wherein the excitation wavelength is 365 nm, the first fluorescence wavelength is 420 nm, and the second fluorescence wavelength is 460 nm.

10. The measuring device according to claim 1, wherein the fluorescence generated from blood vessel tissues in the measurement target, which is excited by the excitation light irradiation unit, is fluorescence attributable to Advanced Glycation Endproducts (AGEs), and the Advanced Glycation Endproducts are accumulated by a physiological reaction in the living body.

11. A measuring system, comprising:

a scanning mechanism including an excitation light irradiation unit for irradiating a measurement target with excitation light, and a light receiving unit for receiving fluorescence generated from the measurement target upon irradiation with the excitation light;

a driving mechanism for controlling relative positions of the scanning mechanism and the measurement target; and

a measuring device according to claim 1, the measuring device communicating with the scanning mechanism and the driving mechanism.

12. A non-transitory computer-readable recording medium recording a control program for causing a computer to function as the individual means of the measuring device according to claim 1.

13. A measuring method, comprising:

a measurement data obtaining step of obtaining, from a scanning mechanism including an excitation light irradiation unit for irradiating a measurement target with excitation light and a light receiving unit for receiving fluorescence generated from the measurement target upon irradiation with the excitation light, measurement data representing optical characteristics of the fluorescence;

a position information obtaining step of obtaining, from a driving mechanism for controlling relative positions of the scanning mechanism and the measurement target, position information indicating an irradiation position where the measurement target is irradiated with the excitation light when the measurement data is obtained; and

a fluorescence characteristic management step of extracting, as a feature variable, fluorescence intensity at a particular florescence wavelength from the measurement data, and of preparing fluorescence characteristic data including the feature variable and the position information,

a fluorescence characteristic analysis step of preparing map information by plotting the feature variable on a map based on the position information; and

an image preparation step of preparing a visualization image to display the map information on a display unit,

wherein the excitation light irradiation unit includes plural types of light sources having different excitation wavelengths,

in the measurement data obtaining step, measurement data that differs per the excitation wavelength is obtained,

in the fluorescence characteristic management step, the fluorescence characteristic data is prepared per the position information and per the excitation wavelength,

in the fluorescence characteristic analysis step, fluorescence intensity in the measurement data linked in correspondence relation with a first excitation wavelength and fluorescence intensity in the measurement data linked in correspondence relation with a second excitation wavelength are compared with each other per the position information, and information indicating that a result of the comparison satisfies a predetermined condition is assigned to the feature variable plotted at a position indicated by the position information, and

in the image preparation step, the visualization image is prepared in a way of highlighting the feature variable to which the information is assigned.