Cell analysis method, cell analysis device, and cell analysis program
First Claim
1. A cell analysis method in a cell analysis device that uses a quantitative optical path length image of a cell colony formed of a number of cells to analyze the cell colony, the method comprising:
- acquiring the quantitative optical path length image of the cell colony by an acquisition unit of the cell analysis device;
extracting a circular shape corresponding to a cell nucleus of a cell in the acquired quantitative optical path length image by an extraction unit of the cell analysis device;
comparing an inner optical path length in an inner region of the extracted circular shape and an outer optical path length in an outer region of the extracted circular shape by a comparison unit of the cell analysis device; and
analyzing the cell colony based on the comparison result by an analysis unit of the cell analysis device to identify a cell with good quality, wherein the outer optical path length of the circular shape is larger than the inner optical path length of the circular shape for the cell with good quality.
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Accused Products
Abstract
Provided is a cell analysis method in a cell analysis device D that uses an optical path length image of a cell colony formed of a large number of cells to analyze the cell colony, the method comprising: acquiring the optical path length image of the cell colony by an acquisition unit of the cell analysis device; extracting a circular shape corresponding to a cell nucleus of the cell in the acquired optical path length image by an extraction unit of the cell analysis device extracts; comparing an inner optical path length of the extracted circular shape and an outer optical path length of the extracted circular shape by a comparison unit of the cell analysis device extracts; and analyzing the cell colony based on the comparison result by analysis unit of the cell analysis device.
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Citations
25 Claims
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1. A cell analysis method in a cell analysis device that uses a quantitative optical path length image of a cell colony formed of a number of cells to analyze the cell colony, the method comprising:
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acquiring the quantitative optical path length image of the cell colony by an acquisition unit of the cell analysis device; extracting a circular shape corresponding to a cell nucleus of a cell in the acquired quantitative optical path length image by an extraction unit of the cell analysis device; comparing an inner optical path length in an inner region of the extracted circular shape and an outer optical path length in an outer region of the extracted circular shape by a comparison unit of the cell analysis device; and analyzing the cell colony based on the comparison result by an analysis unit of the cell analysis device to identify a cell with good quality, wherein the outer optical path length of the circular shape is larger than the inner optical path length of the circular shape for the cell with good quality. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A cell analysis system that uses a quantitative optical path length image of a cell colony formed of a number of cells to analyze the cell colony, the system comprising:
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a microscope, comprising; a stage that supports the cell colony; and a light emission source that irradiates light onto the cell colony mounted on the stage; an objective lens capable of viewing the irradiated cell colony on the stage; a camera that captures at least one image of at least a portion of the irradiated cell colony; and a cell analysis device, comprising; memory configured to store the at least one image captured by the camera; a processor communicatively coupled to the memory, the processor configured to; acquire the quantitative optical path length image of the cell colony; extract a circular shape corresponding to a cell nucleus of a cell in the acquired quantitative optical path length image; compare an inner optical path length in an inner region of the extracted circular shape and an outer optical path length in an outer region of the extracted circular shape; and analyze the cell colony based on the comparison result to identify a cell with good quality, wherein the outer optical path length of the circular shape is larger than the inner optical path length of the circular shape for the cell with good quality.
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9. A non-transitory computer-readable medium containing program instructions for causing a computer to perform the method of:
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in a cell analysis device that uses a quantitative optical path length image of a cell colony formed of a number of cells to analyze the cell colony, acquiring the quantitative optical path length image of the cell colony by an acquisition unit of the cell analysis device; extracting a circular shape corresponding to a cell nucleus of a cell in the acquired quantitative optical path length image by an image extraction unit of the cell analysis device; comparing an inner optical path length in an inner region of the extracted circular shape and an outer optical path length in an outer region of the extracted circular shape by a comparison unit of the cell analysis device; and analyzing the cell colony based on the comparison result by an analysis unit of the cell analysis device to identify a cell with good quality, wherein the outer optical path length of the circular shape is larger than the inner optical path length of the circular shape for the cell with good quality.
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10. A method for analyzing a cell based on quantitative optical path length image of the cell, the method comprising:
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acquiring the optical path length image of the cell; comparing a first quantitative optical path length of a first portion corresponding to a cell nucleus of the cell and a second quantitative optical path length of outside of the first portion; and determining that the cell is a cell with good quality based on the comparison result, wherein the second quantitative optical path length is larger than the first quantitative optical path length for the cell with good quality. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17)
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18. An apparatus for analyzing a cell based on a quantitative optical path length image of the cell, the apparatus comprising:
a computer including a processor and configured to; acquire the quantitative optical path length image of the cell, compare a first optical path length of a first portion corresponding to a cell nucleus of the cell and a second optical path length of outside of the first portion, and determine that the cell is a cell with good quality based on the comparison result, wherein the second optical path length is larger than the first optical path length for the cell with good quality. - View Dependent Claims (19, 20, 21, 22, 23, 24, 25)
Specification