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Method for producing lipids by liberation from biomass

  • US 9,408,404 B2
  • Filed: 10/22/2004
  • Issued: 08/09/2016
  • Est. Priority Date: 05/03/2002
  • Status: Active Grant
First Claim
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1. A method for obtaining a polyunsaturated fatty acid-containing lipid from a biomass, comprising:

  • a) contacting an enzyme and a surfactant with a biomass at a temperature of about 10 degree Celsius to about 80 degree Celsius, and at a pH level of about pH 5 to about pH 9, said enzyme degrading a portion of the biomass impeding recovery of the lipid, and the portion of the biomass is selected from the group consisting of proteins, polysaccharides, cell wall, outer cell membrane, peptidoglycan layer, lipid bilayer, cellulose, chitin, hemicellulose, lignin, and lignin-related compounds,b) recovering said lipid having an anisidine value of 0.8 comprising at least 20 weight percent docosahexaenoic acid, at least 5 weight percent docosapentaenoic acid, and/or at least 20 weight percent arachidonic acid; and

    c) treating the recovered lipid by refining, bleaching, or deodorizing.

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