Immobilised biological entities

• US 9,408,950 B2
• Filed: 07/02/2015
• Issued: 08/09/2016
• Est. Priority Date: 03/11/2011
• Status: Active Grant

• Current Assignee: W. L. Gore & Associates (W.L. Gore & Associates)
• Original Assignee: WL Gore and Associates Incorporated (W.L. Gore and Associates)
• Inventors: Leontein, Karin, Antoni, Per, Nystrm, Daniel, Begovac, Paul, Pietrzak, Krzysztof
• Primary Examiner(s): CHANNAVAJJALA, LAKSHMI SARADA
• Application Number:
  US14/790,205
• Publication Number:
  US 20150367040A1
• Time in Patent Office: 404 Days
• US Class Current: 1/1
• CPC Class Codes: A61L 2300/42 Anti-thrombotic agents, anticoagulants, anti-platelet agents
• CPC Class Codes: A61L 2300/452 Lubricants
• CPC Class Codes: A61L 27/34 Macromolecular materials
• CPC Class Codes: A61L 29/085 Macromolecular materials
• CPC Class Codes: A61L 31/10 Macromolecular materials
• CPC Class Codes: A61L 33/0011 Anticoagulant, e.g. heparin, platelet aggregation inhibitor, fibrinolytic agent, other than enzymes, attached to the substrate
• CPC Class Codes: A61L 33/0035 using a polymer with positively charged atoms in the polymeric backbone, e.g. ionenes
• CPC Class Codes: A61L 33/0064 Hydrogels or hydrocolloids
• CPC Class Codes: A61L 33/0076 Chemical modification of the substrate
• CPC Class Codes: A61L 33/068 obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds A61L33/062 takes precedence
• CPC Class Codes: A61L 33/08 Polysaccharides
• CPC Class Codes: A61P 7/02 Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
• CPC Class Codes: C08G 81/00 Macromolecular compounds obtained by interreacting polymers in the absence of monomers, e.g. block polymers involving only carbon-to-carbon unsaturated bond reactions C08F299/00 ; polyester-amides C08G69/44; polyester-imides C08G73/16; polyamides-imides C08G73/14; block- or graft polymers containing polysiloxane sequences C08G77/42
• CPC Class Codes: C08L 101/005 Dendritic macromolecules
• CPC Class Codes: C08L 79/02 Polyamines
• CPC Class Codes: C08L 79/08 Polyimides; Polyester-imides; Polyamide-imides; Polyamide acids or similar polyimide precursors
• CPC Class Codes: Y10T 428/31971 Of carbohydrate

Abstract

There is described inter alia a device having a surface comprising a layered coating wherein the outer coating layer comprises a plurality of cationic hyperbranched polymer molecules characterized by having (i) a core moiety of molecular weight 14-1,000 Da (ii) a total molecular weight of 1,500 to 1,000,000 Da (iii) a ratio of total molecular weight to core moiety molecular weight of at least 80:1 and (iv) functional end groups, whereby one or more of said functional end groups have an anti-coagulant entity covalently attached thereto.

Claims

1. A cationic hyperbranched polymer molecule characterized by having (i) a core moiety of molecular weight 14-1,000 Da (ii) a total molecular weight of 10,000 to 300,000 Da (iii) a ratio of total molecular weight to core moiety molecular weight of at least 80:

1 and (iv) functional end groups, whereby one or more of said functional end groups have a heparin moiety covalently attached thereto, and wherein the hyperbranched polymer is not a dendrimer.

2. A cationic hyperbranched polymer molecule according to claim 1, wherein the heparin moiety is full length (native) heparin.

3. A cationic hyperbranched polymer molecule according to claim 1, wherein the heparin moiety is nitrous acid degraded heparin.

4. A cationic hyperbranched polymer molecule according to claim 1, wherein the heparin moiety is single point attached to the hyperbranched polymer molecule.

5. A cationic hyperbranched polymer molecule according to claim 1, wherein the heparin moiety is attached to the hyperbranched polymer molecule via the reducing end of the heparin moiety.

6. A cationic hyperbranched polymer molecule according to claim 1 wherein the functional end groups are primary amine groups.

7. A cationic hyperbranched polymer molecule according to claim 1, wherein the hyperbranched polymer has ethylenediamine as core moiety.

8. A cationic hyperbranched polymer molecule according to claim 1, wherein the hyperbranched polymer has a molecular weight of 25,000 to 200,000 Da.

9. A cationic hyperbranched polymer molecule according to claim 1 wherein the ratio of total molecular weight of the hyperbranched polymer to core moiety molecular weight is between 200:

1 and 5000;

1.

10. A cationic hyperbranched polymer molecule according to claim 1 which has a net positive charge.

11. A cationic hyperbranched polymer molecule according to claim 1 which has a net negative charge.

12. A cationic hyperbranched polymer molecule according to claim 9 wherein the ratio of total molecular weight of the hyperbranched polymer to core moiety molecular weight is between 200:

1 and 1600;

1.