Medium comprising transforming growth factor beta 1 and basic fibroblast growth factor
First Claim
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1. A culture medium being devoid of serum and comprising bFGF, TGFβ
- 1 and transferrin, wherein the culture medium is capable of maintaining pluripotent stem cell in an undifferentiated state for at least 5 passages.
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Abstract
The present invention is of methods of establishing and propagating human embryonic stem cell lines using feeder cells-free, xeno-free culture systems and stem cells which are capable of being maintained in an undifferentiated, pluripotent and proliferative state in culture which is free of xeno contaminants and feeder cells.
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Citations
25 Claims
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1. A culture medium being devoid of serum and comprising bFGF, TGFβ
- 1 and transferrin, wherein the culture medium is capable of maintaining pluripotent stem cell in an undifferentiated state for at least 5 passages.
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A culture medium being devoid of serum and comprising bFGF, TGFβ
- 1 and insulin, wherein the culture medium is capable of maintaining pluripotent stem cell in an undifferentiated state.
- View Dependent Claims (14, 15, 16, 17, 18, 19)
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20. A culture medium being devoid of serum and comprising bFGF, TGFβ
- 1, transferrin and insulin, wherein the culture medium is capable of maintaining pluripotent stem cell in an undifferentiated state.
- View Dependent Claims (21, 22, 23, 24, 25)
Specification