Alcohol dehydrogenases (ADH) useful for fermentive production of lower alkyl alcohols
First Claim
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1. A recombinant microbial host cell comprising a biosynthetic pathway for production of isobutanol, the biosynthetic pathway comprising a substrate to product conversion catalyzed by a polypeptide with alcohol dehydrogenase activity, wherein the polypeptide with alcohol dehydrogenase activity has at least 90% identity to the amino acid sequence of SEQ ID NO:
- 36 and a KI value for isobutanol that is higher relative to a control polypeptide having the amino acid sequence of SEQ ID NO;
26.
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Abstract
The invention relates to suitable candidate alcohol dehydrogenase (ADH) enzymes for production of lower alkyl alcohols including isobutanol. The invention also relates to recombinant host cells that comprise such ADH enzymes and methods for producing lower alkyl alcohols in the same.
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Citations
13 Claims
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1. A recombinant microbial host cell comprising a biosynthetic pathway for production of isobutanol, the biosynthetic pathway comprising a substrate to product conversion catalyzed by a polypeptide with alcohol dehydrogenase activity, wherein the polypeptide with alcohol dehydrogenase activity has at least 90% identity to the amino acid sequence of SEQ ID NO:
- 36 and a KI value for isobutanol that is higher relative to a control polypeptide having the amino acid sequence of SEQ ID NO;
26. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
- 36 and a KI value for isobutanol that is higher relative to a control polypeptide having the amino acid sequence of SEQ ID NO;
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11. A method for producing isobutanol comprising:
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(a) providing a recombinant microbial host cell comprising an isobutanol biosynthetic pathway, the pathway comprising a heterologous polypeptide which catalyzes the substrate to product conversion of isobutyraldehyde to isobutanol wherein the polypeptide has at least 90% identity to the amino acid sequence of SEQ ID NO;
36 and a KI value for isobutanol that is higher relative to a control polypeptide having the amino acid sequence of SEQ ID NO;
26,and (b) contacting the host cell of (a) with a carbon substrate under conditions whereby isobutanol is produced. - View Dependent Claims (12, 13)
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Specification