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Alcohol dehydrogenases (ADH) useful for fermentive production of lower alkyl alcohols

  • US 9,410,166 B2
  • Filed: 05/20/2014
  • Issued: 08/09/2016
  • Est. Priority Date: 12/29/2009
  • Status: Active Grant
First Claim
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1. A recombinant microbial host cell comprising a biosynthetic pathway for production of isobutanol, the biosynthetic pathway comprising a substrate to product conversion catalyzed by a polypeptide with alcohol dehydrogenase activity, wherein the polypeptide with alcohol dehydrogenase activity has at least 90% identity to the amino acid sequence of SEQ ID NO:

  • 36 and a KI value for isobutanol that is higher relative to a control polypeptide having the amino acid sequence of SEQ ID NO;

    26.

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