Sugar mixtures and methods for production and use thereof

• US 9,410,216 B2
• Filed: 06/26/2011
• Issued: 08/09/2016
• Est. Priority Date: 06/26/2010
• Status: Active Grant

• Current Assignee: Virdia Ltd
• Original Assignee: Virdia, Inc. (Stora Enso Oyj)
• Inventors: Eyal, Aharon Meir, Jansen, Robert P., Mali, Revital, Vitner, Asher
• Primary Examiner(s): Heincer, Liam J
• Application Number:
  US13/378,657
• Publication Number:
  US 20120264873A1
• Time in Patent Office: 1,871 Days
• Field of Search: 127/30, 127/37
• US Class Current: 1/1
• CPC Class Codes: A23V 2002/00 Food compositions, function of food ingredients or processes for food or foodstuffs
• CPC Class Codes: A23V 2250/60 Sugars, e.g. mono-, di-, tri-, tetra-saccharides
• CPC Class Codes: C01B 7/0737 hydrogen chloride being extracted
• CPC Class Codes: C12N 1/22 Processes using, or culture media containing, cellulose or hydrolysates thereof
• CPC Class Codes: C12P 19/02 Monosaccharides 2-ketogulonic acid C12P7/60
• CPC Class Codes: C12P 19/14 produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase , e.g. by cellulase, hemicellulase
• CPC Class Codes: C12P 2203/00 Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source ethanol C12P7/10
• CPC Class Codes: C12P 7/10 substrate containing cellulosic material
• CPC Class Codes: C13K 1/02 obtained by saccharification of cellulosic materials manufacture of fodder A23K10/32
• CPC Class Codes: C13K 13/00 Sugars not otherwise provided for in this class
• CPC Class Codes: C13K 7/00 Maltose
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

A sugar mixture comprising: monosaccharides; oligosaccharides in a ratio ≧0.06 to total saccharides; disaccharides in a ratio to total saccharides ≧0.05; pentose in a ratio to total saccharides ≧0.05; at least one alpha-bonded di-glucose; and at least one beta-bonded di-glucose. Also disclosed are methods to make and/or use such mixtures.

Claims

1. A de-acidified and de-salted sugar mixture, the de-acidified and de-salted sugar mixture comprising monosaccharide hydrolysates;

(i) higher oligosaccharide hydrolysates comprising three or more sugar units;

(ii) a ratio of disaccharide hydrolysates to total saccharides of ≧

0.05 on a weight by weight basis;

(iii) a ratio of pentose to total saccharides of ≧

0.05 on a weight by weight basis;

(iv) at least one alpha-bonded di-glucose;

(v) at least one beta-bonded di-glucose; and

(vi) a mineral acid to carbohydrate ratio of less than 0.01 on a weight by weight basis.

2. A mixture according to claim 1, having a ratio of higher oligosaccharide hydrolysates to total saccharides of ≦

0.2 on a weight by weight basis.

3. A mixture according to claim 1, wherein a ratio of at least one of the alpha-bonded di-glucose and the beta-bonded di-glucose relative to total saccharides is ≧

0.01 on a weight by weight basis.

4. A mixture according to claim 3, wherein a ratio of at least one of the alpha-bonded di-glucose and the beta-bonded di-glucose relative to total saccharides is ≧

0.03 on a weight by weight basis.

5. A mixture according to claim 1, wherein the alpha-bonded di-glucose includes at least one member selected from the group consisting of maltose, isomaltose and trehalose.

6. A mixture according to claim 1, wherein the beta-bonded di-glucose includes at least one member selected from the group consisting of gentiobiose, sophorose and cellobiose.

7. A mixture according to claim 1, wherein the mineral acid to carbohydrate ratio is less than 0.005 on a weight by weight basis.

8. A mixture according to claim 1, wherein the sugar mixture comprises oligosaccharide hydrolysates in a ratio to total saccharides of ≧

0.06 on a weight by weight basis.

9. A mixture according to claim 8, wherein the oligosaccharide hydrolysates comprise two or more sugar units.

10. A mixture according to claim 1, wherein the sugar mixture further comprises a water-soluble organic compound impurity.

11. A mixture according to claim 1, wherein the oligosaccharide hydrolysates to total carbohydrate ratio is less than 0.1 on a weight by weight basis.

12. A method comprising:

(a) providing a fermentor; and

(b) fermenting a medium comprising a de-acidified and de-salted sugar mixture according to claim 1 in the fermentor to produce a fermentation product.

13. A method according to claim 12, wherein the fermentation product includes at least one member selected from the group consisting of alcohols, carboxylic acids, amino acids, monomers for the polymer industry and proteins.

14. A method according to claim 12, comprising processing the fermentation product to produce a consumer product.

15. A method according to claim 12, wherein the fermentation product includes at least one member selected from the group consisting of ethanol, butanol, isobutanol, a fatty acid, a fatty acid ester, a fatty alcohol and biodiesel.

16. A method according to claim 15, comprising processing of the fermentation product.

17. The method of claim 12, further comprising converting the fermentation product to a consumer product, a precursor of a consumer product, or an ingredient of a consumer product produced.

18. The method of claim 17, comprising converting the fermentation product to a consumer product.

19. The method of claim 17, comprising converting the fermentation product to a consumer product, a precursor of a consumer product, or an ingredient of a consumer product, wherein the consumer product has a ratio of carbon-14 to carbon-12 of about 2.0×

10<sup>−

13</sup> or greater.

20. The method of claim 17, comprising converting the fermentation product to a consumer product, wherein the consumer product further comprises an additional ingredient produced from a raw material other than lignocellulosic material.

21. The method of claim 17, comprising converting the fermentation product to a consumer product, wherein the consumer product comprises a marker molecule at a concentration of at least 100 ppb.

22. A method according to claim 12, wherein a sugar mixture prior to de-acidification and de-salting comprises 1.5 times more salt as compared to the de-acidified and de-salted sugar mixture.

23. A method according to claim 12, wherein the sugar mixture further comprises a water-soluble organic compound impurity.

24. A method according to claim 23, wherein the water-soluble organic compound impurity comprises multiple compounds.

25. A method according to claim 23, wherein a sugar mixture prior to de-acidification and de-salting comprises 1.5 times more water-soluble organic compound impurity as compared to the de-acidified and de-salted sugar mixture.

26. A de-acidified and de-salted sugar mixture, the de-acidified and de-salted sugar mixture comprising monosaccharide hydrolysates and oligosaccharide hydrolysates, wherein the oligosaccharide hydrolysates to total carbohydrate in the de-acidified and de-salted sugar mixture has a ratio of less than 0.15 on a weight by weight basis;

wherein the de-acidified and de-salted sugar mixture is further characterized by having;

(i) higher oligosaccharide hydrolysates comprising three or more sugar units;

(ii) a ratio of disaccharide hydrolysates to total saccharides of ≧

0.05 on a weight by weight basis;

(iii) a ratio of pentose to total saccharides of ≧

0.05 on a weight by weight basis;

(iv) at least one alpha-bonded di-glucose;

(v) at least one beta-bonded di-glucose; and

(vi) sulfuric acid to carbohydrate ratio of less than 0.01 on a weight by weight basis.

27. A mixture according to claim 26, wherein the oligosaccharide hydrolysates to total carbohydrate ratio is less than 0.1 on a weight by weight basis.