Method of using a biosensor
First Claim
1. A method of using a biosensor including a mixture of an only one enzyme, a mediator, and an oxidizable species as an internal reference, said oxidizable species being different than the mediator species and having different redox potentials, wherein said mixture is located directly on a surface of at least a portion of a working electrode, said method comprising the steps of:
- applying a first voltage potential in a first voltage period;
providing a set delay period;
applying a second voltage potential in a second voltage period following said delay period; and
wherein said first voltage potential and said second voltage potential are selectively provided for oxidizing only said mediator or both said mediator and said internal reference.
2 Assignments
0 Petitions
Accused Products
Abstract
A biosensor (102) for determining the presence or amount of a substance in a sample and methods of use of the biosensor (102) are provided. The biosensor (102) for receiving a user sample to be analyzed includes a mixture for electrochemical reaction with an analyte. The mixture includes an enzyme, a mediator and an oxidizable species as an internal reference.
674 Citations
26 Claims
-
1. A method of using a biosensor including a mixture of an only one enzyme, a mediator, and an oxidizable species as an internal reference, said oxidizable species being different than the mediator species and having different redox potentials, wherein said mixture is located directly on a surface of at least a portion of a working electrode, said method comprising the steps of:
-
applying a first voltage potential in a first voltage period; providing a set delay period; applying a second voltage potential in a second voltage period following said delay period; and
wherein said first voltage potential and said second voltage potential are selectively provided for oxidizing only said mediator or both said mediator and said internal reference. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
-
-
15. A method of using a biosensor to assist in determining glucose concentration, the method comprising:
-
providing a biosensor including a reagent, located directly on a surface of at least a portion of a working electrode, the reagent including an only one enzyme, mediator and an oxidizable species as an internal reference, said oxidizable species being different than the mediator species and having different redox potentials; applying a first voltage potential in a first voltage period; providing a set delay period; and applying a second voltage potential in a second voltage period following said delay period, wherein said first voltage potential is selectively provided for oxidizing only said mediator or both said mediator and said internal reference, and wherein said second voltage potential is selectively provided for oxidizing only said mediator or both said mediator and said internal reference. - View Dependent Claims (16, 17, 18, 19, 20)
-
-
21. A method of using a biosensor to assist in determining an analyte concentration, the method comprising:
-
providing a biosensor including a reagent, the reagent, located directly on a surface of at least a portion of the working electrode, including only one enzyme, mediator and an oxidizable species as an internal reference; applying a first voltage potential in a first voltage period; providing a set delay period; and
applying a second voltage potential in a second voltage period following said delay period;wherein one of said first voltage potential and said second voltage potential oxidizes only said mediator and the other one of said first voltage potential and said second voltage potential oxidizes both said mediator and said internal reference. - View Dependent Claims (22, 23, 24, 25, 26)
-
Specification