System and method for removing a cryporotectant from a liquid
First Claim
1. A system for removing cryoprotectant from a cryoprotectant-containing liquid stored in a container, the system comprising:
- a dialyzer having a first inlet to receive the cryoprotectant-containing liquid, a first outlet for discharge of the cryoprotectant-containing liquid, a second inlet to receive a cryoprotectant-free dialysate liquid and a second outlet for discharge of the cryoprotectant-free liquid, said dialyzer forming a diffusion gradient between said cryoprotectant-containing liquid and the cryoprotectant-free liquid;
a first fluid circuit between the container of the cryo-protectant containing liquid and said dialyzer, said first fluid circuit including;
a first fluid line connected to said first inlet and connectable to an outlet of the container;
a first valve disposed in said first fluid line, said first valve openable to permit and closable to prevent flow of cryoprotectant-containing liquid from the container to said dialyzer;
an inlet fluid line connected to said first outlet of said dialyzer and connectable to an inlet of the container; and
a first pump configured to pump liquid through said first fluid circuit in a first direction from said inlet of said dialyzer to said first outlet of said dialyzer, said first pump also configured to pump liquid through said first fluid circuit in a second direction from said first outlet of said dialyzer to said inlet of said dialyzer;
a source of cryoprotectant-free dialysate liquid capable of receiving cryoprotectant transferred through said diffusion gradient within said dialyzer;
a second fluid circuit between the source of dialysate liquid and said dialyzer, said second fluid circuit including;
a second fluid line connected between said source of dialysate liquid and said second inlet of said dialyzer;
a second valve disposed in said second fluid line, said second valve openable to permit and closable to prevent flow of dialysate liquid from said source to said dialyzer; and
a discharge fluid line connected to said second outlet of said dialyzer and connectable to a waste container;
a source of a priming solution for preparing said dialyzer;
a priming fluid circuit between said source of priming solution and said dialyzer, said priming fluid circuit including;
a first priming fluid line connected between said source of priming solution and said first inlet of said dialyzer;
a third valve disposed in said first priming fluid line, said third valve openable to permit and closable to prevent flow of priming solution from said source of priming solution to said dialyzer; and
a second priming fluid line connected between said source of priming solution and said second inlet of said dialyzer and a fourth valve disposed in said second priming fluid line, said fourth valve openable to permit and closable to prevent flow of priming solution from said source of priming solution to said dialyzer;
a differential conductivity device disposed between said second fluid line and said discharge fluid line and operable to measure the difference in conductivity between dialysate liquid flowing through said second fluid line and dialysate liquid that has received cryoprotectant transferred by said dialyzer flowing through said discharge fluid line; and
a controller for controlling the opening and closing of said first, second, third and fourth, valves in response to the measured difference in conductivity, and wherein said controller is operable to control the direction of flow from said first pump in response to the measured difference in conductivity.
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Accused Products
Abstract
A system for removing cryoprotectant from a cryoprotectant-containing liquid stored a container comprises a cryoprotectant removal device that receives the cryoprotectant-containing liquid and a cryoprotectant-free dialysate liquid and that is operable to transfer cryoprotectant to the dialysate liquid. A differential conductivity device is arranged to continuously measure the difference in conductivity between dialysate liquid entering the device and dialysate liquid that has received cryoprotectant transferred by the dialyzer discharged from the device. A controller is operable to control the flow of the liquids through the device in response to the measured difference in conductivity, and particularly to stop the flow of the cryoprotectant-containing liquid when the measured differential conductivity indicates that the cryoprotectant has been substantially removed from the liquid.
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Citations
15 Claims
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1. A system for removing cryoprotectant from a cryoprotectant-containing liquid stored in a container, the system comprising:
- a dialyzer having a first inlet to receive the cryoprotectant-containing liquid, a first outlet for discharge of the cryoprotectant-containing liquid, a second inlet to receive a cryoprotectant-free dialysate liquid and a second outlet for discharge of the cryoprotectant-free liquid, said dialyzer forming a diffusion gradient between said cryoprotectant-containing liquid and the cryoprotectant-free liquid;
a first fluid circuit between the container of the cryo-protectant containing liquid and said dialyzer, said first fluid circuit including; a first fluid line connected to said first inlet and connectable to an outlet of the container; a first valve disposed in said first fluid line, said first valve openable to permit and closable to prevent flow of cryoprotectant-containing liquid from the container to said dialyzer; an inlet fluid line connected to said first outlet of said dialyzer and connectable to an inlet of the container; and a first pump configured to pump liquid through said first fluid circuit in a first direction from said inlet of said dialyzer to said first outlet of said dialyzer, said first pump also configured to pump liquid through said first fluid circuit in a second direction from said first outlet of said dialyzer to said inlet of said dialyzer; a source of cryoprotectant-free dialysate liquid capable of receiving cryoprotectant transferred through said diffusion gradient within said dialyzer; a second fluid circuit between the source of dialysate liquid and said dialyzer, said second fluid circuit including; a second fluid line connected between said source of dialysate liquid and said second inlet of said dialyzer; a second valve disposed in said second fluid line, said second valve openable to permit and closable to prevent flow of dialysate liquid from said source to said dialyzer; and a discharge fluid line connected to said second outlet of said dialyzer and connectable to a waste container; a source of a priming solution for preparing said dialyzer; a priming fluid circuit between said source of priming solution and said dialyzer, said priming fluid circuit including; a first priming fluid line connected between said source of priming solution and said first inlet of said dialyzer; a third valve disposed in said first priming fluid line, said third valve openable to permit and closable to prevent flow of priming solution from said source of priming solution to said dialyzer; and a second priming fluid line connected between said source of priming solution and said second inlet of said dialyzer and a fourth valve disposed in said second priming fluid line, said fourth valve openable to permit and closable to prevent flow of priming solution from said source of priming solution to said dialyzer; a differential conductivity device disposed between said second fluid line and said discharge fluid line and operable to measure the difference in conductivity between dialysate liquid flowing through said second fluid line and dialysate liquid that has received cryoprotectant transferred by said dialyzer flowing through said discharge fluid line; and a controller for controlling the opening and closing of said first, second, third and fourth, valves in response to the measured difference in conductivity, and wherein said controller is operable to control the direction of flow from said first pump in response to the measured difference in conductivity. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
- a dialyzer having a first inlet to receive the cryoprotectant-containing liquid, a first outlet for discharge of the cryoprotectant-containing liquid, a second inlet to receive a cryoprotectant-free dialysate liquid and a second outlet for discharge of the cryoprotectant-free liquid, said dialyzer forming a diffusion gradient between said cryoprotectant-containing liquid and the cryoprotectant-free liquid;
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11. A system for removing cryoprotectant from a cryoprotectant-containing liquid the system comprising:
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a dialyzer having a first inlet to receive the cryoprotectant-containing liquid, a first outlet for discharge of the cryoprotectant-containing liquid, a second inlet to receive a cryoprotectant-free dialysate liquid and a second outlet for discharge of the cryoprotectant-free liquid, said dialyzer forming a diffusion gradient between said cryoprotectant-containing liquid and the cryoprotectant-free liquid; a container having an inlet port and an outlet port, said container containing a cryoprotectant-containing liquid; a first fluid circuit between the container and said dialyzer, said first fluid circuit including; a first fluid line connected to said first inlet and connectable to said outlet port of said container; a first valve disposed in said first fluid line, said first valve openable to permit and closable to prevent flow of cryoprotectant-containing liquid from said container to said dialyzer; and an inlet fluid line connected to said first outlet of said dialyzer and connectable to said inlet port of said container; and a first pump configured to pump liquid through said first fluid circuit in a first direction from said inlet of said dialyzer to said first outlet of said dialyzer, said first pump also configured to pump liquid through said first fluid circuit in a second direction from said first outlet of said dialyzer to said inlet of said dialyzer; a source of cryoprotectant-free dialysate liquid capable of receiving cryoprotectant transferred through said diffusion gradient within said dialyzer; a second fluid circuit between the source of dialysate liquid and said dialyzer, said second fluid circuit including; a second fluid line connected between said source of dialysate liquid and said second inlet of said dialyzer; a second valve disposed in said second fluid line, said second valve openable to permit and closable to prevent flow of dialysate liquid from said source to said dialyzer; and a discharge fluid line connected to said second outlet of said dialyzer and connectable to a waste container; a source of a priming solution for preparing said dialyzer; a priming fluid circuit between said source of priming solution and said dialyzer, said priming fluid circuit including; a first priming fluid line connected between said source of priming solution and said first inlet of said dialyzer; a third valve disposed in said first priming fluid line, said third valve openable to permit and closable to prevent flow of priming solution from said source of priming solution to said dialyzer; and a second priming fluid line connected between said source of priming solution and said second inlet of said dialyzer and a fourth valve disposed in said second priming fluid line, said fourth valve openable to permit and closable to prevent flow of priming solution from said source of priming solution to said dialyzer; a differential conductivity device disposed between said second fluid line and said discharge fluid line and operable to measure the difference in conductivity between dialysate liquid flowing through said second fluid line and dialysate liquid that has received cryoprotectant transferred by said dialyzer flowing through said discharge fluid line; and a controller for controlling the opening and closing of said first, second, third and fourth valves in response to the measured difference in conductivity, and wherein said controller is operable to control the direction of flow from said first pump in response to the measured difference in conductivity, such that said first pump is operable to return a portion of said cryoprotectant containing liquid remaining in said first fluid line to said container by pushing said portion of said cryoprotectant containing liquid in said second direction through said outlet port of said container. - View Dependent Claims (12, 13, 14, 15)
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Specification