Devices and systems for creation of DNA cluster arrays
First Claim
Patent Images
1. An assembly for creating nucleic acid cluster arrays, the assembly comprising:
- a flow cell having a bottom side and a top side that face in opposite directions, the flow cell including an inlet hole and an outlet hole along the top side and a flow channel that fluidicly couples the inlet and outlet holes, the flow channel being defined by a surface that includes a plurality of oligonucleotides immobilized thereto for nucleic acid cluster generation; and
a disposable manifold that is separable from the flow cell, the manifold including a main body configured to be mounted onto the flow cell, the main body comprising an outer side and an under side that face in opposite directions, the under side including an input port and an output port that align with the inlet hole and the outlet hole of the flow cell, respectively, when the under side is mounted onto the top side of the flow cell during the nucleic acid cluster generation, the manifold also including an input tube and an output tube that are coupled to the main body and in flow communication with the input and output ports, respectively, wherein fluid flows from the input tube, through the flow channel, and through the outlet tube during the nucleic acid cluster generation.
2 Assignments
0 Petitions
Accused Products
Abstract
The present invention comprises systems and devices for isothermal amplification of polynucleotide sequences to produce DNA cluster arrays.
-
Citations
21 Claims
-
1. An assembly for creating nucleic acid cluster arrays, the assembly comprising:
-
a flow cell having a bottom side and a top side that face in opposite directions, the flow cell including an inlet hole and an outlet hole along the top side and a flow channel that fluidicly couples the inlet and outlet holes, the flow channel being defined by a surface that includes a plurality of oligonucleotides immobilized thereto for nucleic acid cluster generation; and a disposable manifold that is separable from the flow cell, the manifold including a main body configured to be mounted onto the flow cell, the main body comprising an outer side and an under side that face in opposite directions, the under side including an input port and an output port that align with the inlet hole and the outlet hole of the flow cell, respectively, when the under side is mounted onto the top side of the flow cell during the nucleic acid cluster generation, the manifold also including an input tube and an output tube that are coupled to the main body and in flow communication with the input and output ports, respectively, wherein fluid flows from the input tube, through the flow channel, and through the outlet tube during the nucleic acid cluster generation. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
-
-
12. A system for creating nucleic acid cluster arrays, the system comprising:
-
a system body having a placement area configured to receive a flow cell; a temperature control device configured to regulate a temperature of the flow cell, the temperature control device including, or being in thermal communication with, the placement area; a fluid flow system including at least one pump, the fluid flow system configured to direct fluid through the flow cell during nucleic acid cluster generation; a computer control component functionally connected to the temperature control device and the fluid flow system, the computer control component configured to control the temperature control device and the fluid flow system to conduct the nucleic acid cluster generation; and a flow cell holder that is configured to move between open and closed positions, the flow cell holder permitting access to the placement area when in the open position for placing the flow cell on the placement area and placing a manifold onto the flow cell, the flow cell holder configured to press the manifold against the flow cell when in the closed position so that the manifold presses the flow cell toward the flow cell placement area. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20, 21)
-
Specification