Slip chip device and methods

• US 9,415,392 B2
• Filed: 03/23/2010
• Issued: 08/16/2016
• Est. Priority Date: 03/24/2009
• Status: Active Grant

• Current Assignee: University of Chicago
• Original Assignee: University of Chicago
• Inventors: Ismagilov, Rustem F., Du, Wenbin, Li, Liang, Shen, Feng, Nichols, Kevin Paul Flood, Chen, Delai, Kreutz, Jason Eugene
• Primary Examiner(s): HURST, JONATHAN M
• Application Number:
  US13/257,811
• Publication Number:
  US 20120028342A1
• Time in Patent Office: 2,338 Days
• US Class Current: 1/1
• CPC Class Codes: B01F 33/304 the mixing being performed in a mixing chamber where the products are brought into contact
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2300/0609 Holders integrated in container to position an object
• CPC Class Codes: B01L 2300/0809 rectangular shaped
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0861 Configuration of multiple channels and/or chambers in a single devices
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2300/0893 having a very large number of wells, microfabricated wells
• CPC Class Codes: B01L 2300/12 Specific details about materials
• CPC Class Codes: B01L 2300/168 Specific optical properties, e.g. reflective coatings
• CPC Class Codes: B01L 2400/065 sliding valves
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: C12Q 1/025 for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics antimicrobial activity C12Q1/18
• CPC Class Codes: C12Q 1/703 : Viruses associated with AIDS
• CPC Class Codes: G01N 21/78 : producing a change of colour
• CPC Class Codes: G01N 33/54386 : Analytical elements
• CPC Class Codes: H01L 2924/00 : Indexing scheme for arrangements or methods for connecting or disconnecting semiconductor or solid-state bodies as covered by H01L24/00
• CPC Class Codes: H01L 2924/0002 : Not covered by any one of groups H01L24/00, H01L24/00 and H01L2224/00

Abstract

A device is described having a first surface having a plurality of first areas and a second surface having a plurality of second areas. The first surface and the second surface are opposed to one another and can move relative to each other from at least a first position where none of the plurality of first areas, having a first substance, are exposed to plurality of second areas, having a second substance, to a second position. When in the second position, the plurality of first and second areas, and therefore the first and second substances, are exposed to one another. The device may further include a series of ducts in communication with a plurality of first second areas to allow for a substance to be disposed in, or upon, the plurality of second areas when in the first position.

Claims

1. A reaction system for carrying out a reaction, the reaction system comprising:

a first part having a first surface;

a plurality of first areas located along a portion of the first surface, each of the plurality of first areas configured to maintain at least one first substance;

a second part having a second surface opposed to the first surface;

a plurality of second areas located along a portion of the second surface, each of the plurality of second areas configured to maintain at least one second substance; and

an inlet duct,

wherein at least one of the first surface of the first part and the second surface of the second part is configured to move relative to the other between a first position wherein the plurality of first areas are not exposed to any of the plurality of second areas, and a second position, wherein at least one of the plurality of first areas is only exposed to one of the plurality of the second areas, wherein the inlet duct is in fluidic communication via a continuous fluidic path within said first and second parts with each of said plurality of first areas only in said first position or only in said second position, and wherein the first part and the second part are engaged with each other before and after the relative motion.

2. The reaction system of claim 1, wherein the reaction system is in the first position and at least one of the plurality of first areas contains a reagent.

3. The reaction system of claim 1, wherein the reaction system is in the first position and at least one of the plurality of second areas contains a reagent.

4. The reaction system of claim 2 or 3, wherein the reagent comprises at least one component selected from the group consisting of a nucleic acid, an enzyme, a polymerase enzyme, an antibody, a bacterium, a crystallization agent, a protein, a peptide, and a mammalian cell.

5. The reaction system of claim 2 or 3, further comprising a fluid that is immiscible with the reagent.

6. The reaction system of claim 1, wherein the at least one surface of the first part and the second surface of the second part is configured to move relative to the other from the first position to the second position.

7. The reaction system of claim 1, wherein the at least one of the first surface of the first part and the second surface of the second part is configured to move relative to the other from the second position to the first position.

8. The reaction system of claim 1, further comprising:

a third part having a third surface,

a fourth surface on the second part,

wherein a portion of the plurality of second areas is located along the fourth surface, and the third surface is opposed to the fourth surface, and

at least one third area located along a portion of the third surf ace, the at least one third area configured to maintain at least one third substance, wherein at least one of the third surface of the third part and the fourth surface of the second part is configured to move relative to the other between a third position, where the at least one third area is not exposed to the plurality of second areas, and a fourth position, where one of

the at least one third areas is only exposed to one of the plurality of second areas, to form a closed system.

9. The reaction system of claim 1, wherein the at least one of the first surface of the first part and the second surface of the second part is configured to move in a direction substantially perpendicular to the normal of the first surface of the first part when moving from the first position to the second position.

10. The reaction system of claim 1, wherein when in the second position, one of the plurality of second areas is simultaneously exposed to at least two of the plurality of first areas.

11. The reaction system of claim 1, wherein said plurality of first areas is arranged in a first pattern and wherein said plurality of second areas is arranged in a complementary pattern, wherein the movement from the first position to the second position allows at least one of the plurality of first areas to be exposed to a respective second area.

12. The reaction system of claim 11, wherein either the number of first areas equals the number of second areas, the number of first areas is greater than the number of second areas or the number of first areas is less than the number of second areas.

13. The reaction system for carrying out a reaction of claim 1, wherein at least two of the first and second areas differ in volume.

14. The reaction system device of claim 1, further comprising a substrate disposed between the first surface and the second surface.

15. The reaction system of claim 1, wherein at least one of the plurality of first and second areas comprises a well.

16. The reaction system of claim 1, wherein at least one of the plurality of first and second areas comprises a surface pattern.

17. The reaction system of claim 1, wherein at least two areas on the same surface differ in depth.

18. The reaction system of claim 1, wherein the at least one of the first surface of the first part and the second surface of the second part is configured to slide relative to the other either from the first position to the second position, from the second position to the first position, or back and forth between the first and second positions.

19. The reaction system of claim 1,

further comprising an intermediate third part disposed between the first surface of the first part and the second surf ace of the second part, the intermediate third part having an opening formed therethrough;

wherein the first part, the second part and the intermediate third part are configured to slide relative to one another, from the first position where the plurality of first areas is not exposed to the plurality of second areas via the opening to the second position where at least one of the plurality of first areas is exposed to at least one of the plurality of second areas via the opening.

20. The reaction system of claim 1, wherein at least one of said plurality of first areas and at least one of said plurality of second areas together form a closed system in said second position.

21. The reaction system of claim 1, wherein said inlet duct is in a branch-like formation.