Instruments for biological sample-to-answer devices

• US 9,416,343 B2
• Filed: 11/01/2013
• Issued: 08/16/2016
• Est. Priority Date: 11/05/2012
• Status: Active Grant

• Current Assignee: California Institute of Technology
• Original Assignee: California Institute of Technology
• Inventors: Malik, Imran R., Scherer, Axel, Garcia, Erika F., Madero, Xiomara L.
• Primary Examiner(s): Wecker, Jennifer
• Application Number:
  US14/070,440
• Publication Number:
  US 20140127796A1
• Time in Patent Office: 1,019 Days
• US Class Current: 1/1
• CPC Class Codes: B01L 2200/04 Exchange or ejection of cartridges, containers or reservoirs
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2200/147 Employing temperature sensors
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/022 Transponder chips
• CPC Class Codes: B01L 2300/023 Sending and receiving of information, e.g. using bluetooth
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0861 Configuration of multiple channels and/or chambers in a single devices
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2300/1827 using resistive heater
• CPC Class Codes: B01L 2400/0478 pistons
• CPC Class Codes: B01L 3/0275 Interchangeable or disposable dispensing tips
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: B01L 9/527 for microfluidic devices, e.g. used for lab-on-a-chip
• CPC Class Codes: C12M 21/18 Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes

Abstract

Methods and devices for biological sample preparation and analysis are disclosed. A device may have a linear or circular arrangement of containers, with a connecting structure such as a bar or disk. Fluidics channels between containers allow the performance of different techniques for sample preparation, such as lysing, washing and elution. Different functional elements, such as grinders or mixers, may be attached to the containers.

Claims

1. A portable instrument comprising:

a portable housing structure;

a sample-to-answer device housing element, the sample-to-answer device housing element positioned and configured to attach to a sample-to-answer device, the sample to answer device having a reaction cartridge;

a moving plate, positioned and directly adjacent to the sample-to-answer device housing element and configured to attach to the sample-to-answer device housing element;

a solenoid, comprising a first end and a second end, the first end secured to the portable housing structure, the second end secured to the moving plate, wherein the solenoid is positioned and directly adjacent to the moving plate and configured to move the moving plate based on an input of a user to position the reaction cartridge in a correct position for optical interrogation of a target region of the reaction cartridge;

at least one light source, configured to emit a first set of electromagnetic waves in a desired wavelength range;

a waveguide element, attached directly below the at least one light source and configured to focus and direct the first set of electromagnetic waves onto the reaction cartridge of the sample-to-answer device housing element, such that a second set electromagnetic waves, which is resultant from the first set electromagnetic waves hitting the target region of the reaction cartridge, is configured to be emitted from the target region of the reaction cartridge, said waveguide element configured to irradiate a prism of the reaction cartridge of the sample-to-answer device;

at least one detector, positioned and directly adjacent to the waveguide element and the light source and configured to receive the second set of electromagnetic waves from a reaction chamber of the reaction cartridge; and

a printed circuit board directly fixed to the portable housing structure, comprising electronic components configured to control the operation of the solenoid and thereby configured to operate the moving plate, configured to activate the at least one light source, and configured to receive data from the at least one detector, based on the input from the user wherein the at least one light source, the waveguide element, and the at least one detector are each directly fixed to the portable housing structure and positioned adjacent the sample-to-answer device housing element so as together form an integrated instrument.

2. The instrument of claim 1, wherein the solenoid is a bistable solenoid.

3. The instrument of claim 1, wherein the at least one light source is an LED.

4. The instrument of claim 1, further comprising:

at least one motorized actuator configured to attach to a plunger of the sample-to-answer device,

wherein the printed circuit board is further configured to operate the at least one motorized actuator.

5. The instrument of claim 4, wherein the sample-to-answer device housing element is for containers in the sample-to-answer device arranged in a linear pattern.

6. The instrument of claim 4, wherein the sample-to-answer device housing element is for containers in the sample-to-answer device arranged in a circular pattern.

7. The instrument of claim 4, wherein the at least one motorized actuator comprises:

a motor, the motor comprising a rotating rod having a rotating axis;

a fixed plate attached to the rotating axis; and

a linear actuator having a first end and a second end, wherein the first end is attached to the fixed plate, and the second end is attached to a second rod, the second rod having an axis parallel to the rotating axis of the motor, and wherein the second rod is configured to operate the plungers.

8. The instrument of claim 7, wherein the sample-to-answer device has a top structure with a first plurality of plungers and a bottom structure with a second plurality of plungers, and the instrument comprises a plunger for each of the first and second plurality of plungers.

9. The instrument of claim 1, further comprising a filter excitation element attached to the waveguide element.

10. The instrument of claim 4, further comprising a filter excitation element attached to the waveguide element.

11. The instrument of claim 1, wherein the at least one light source and the waveguide element are fabricated in a single piece.

12. The instrument of claim 4, wherein the at least one light source and the waveguide element are fabricated in a single piece.

13. The instrument of claim 9, wherein the at least one light source, the filter excitation element and the waveguide element are fabricated in a single piece.

14. The instrument of claim 10, wherein the at least one light source, the filter excitation element and the waveguide element are fabricated in a single piece.

15. The instrument of claim 1, wherein the instrument can be operated through a smartphone, a tablet, a laptop or a desktop computer.

16. The instrument of claim 4, wherein the instrument can be operated through a smartphone, a tablet, a laptop or a desktop computer.

17. The instrument of claim 1, wherein the instrument can be operated to perform qPCR.

18. The instrument of claim 1, wherein the sample-to-answer device housing element is configured to house a qPCR cartridge.

19. The instrument of claim 1, wherein the at least one light source and the waveguide element are configured to perform optical analysis for qPCR techniques.

20. The instrument of claim 1, wherein the prism is configured to focus and distribute light onto the reaction cartridge when an optical detection technique is carried out.