Methods and compositions for weed control
First Claim
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1. A method of plant control comprising:
- treating a plant with a composition comprising a non-transcribable polynucleotide and a transfer agent, wherein said non-transcribable polynucleotide is from 18 to about 700 nucleotides in length and is at least 85% identical or at least 85% complementary to a PPG oxidase gene sequence, or to an RNA transcript of said PPG oxidase gene sequence wherein said PPG oxidase gene sequence is selected from the group consisting of SEQ ID NOs;
1-5 and 8-71 and a polynucleotide fragment thereof, wherein said transfer agent conditions the surface of said plant for permeation by said non-transcribable polynucleotide, and whereby said plant'"'"'s growth, development, or reproductive ability is suppressed or delayed or said plant is more sensitive to a PPG oxidase inhibitor herbicide as a result of said non-transcribable polynucleotide containing composition relative to a plant not treated with said composition.
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Abstract
Novel compositions for use to enhance weed control. Specifically, the present invention provides for methods and compositions that modulate protoporphyrinogen IX oxidase in weed species. The present invention also provides for combinations of compositions and methods that enhance weed control.
341 Citations
29 Claims
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1. A method of plant control comprising:
- treating a plant with a composition comprising a non-transcribable polynucleotide and a transfer agent, wherein said non-transcribable polynucleotide is from 18 to about 700 nucleotides in length and is at least 85% identical or at least 85% complementary to a PPG oxidase gene sequence, or to an RNA transcript of said PPG oxidase gene sequence wherein said PPG oxidase gene sequence is selected from the group consisting of SEQ ID NOs;
1-5 and 8-71 and a polynucleotide fragment thereof, wherein said transfer agent conditions the surface of said plant for permeation by said non-transcribable polynucleotide, and whereby said plant'"'"'s growth, development, or reproductive ability is suppressed or delayed or said plant is more sensitive to a PPG oxidase inhibitor herbicide as a result of said non-transcribable polynucleotide containing composition relative to a plant not treated with said composition. - View Dependent Claims (2, 3, 4, 5, 6, 7, 26, 28)
- treating a plant with a composition comprising a non-transcribable polynucleotide and a transfer agent, wherein said non-transcribable polynucleotide is from 18 to about 700 nucleotides in length and is at least 85% identical or at least 85% complementary to a PPG oxidase gene sequence, or to an RNA transcript of said PPG oxidase gene sequence wherein said PPG oxidase gene sequence is selected from the group consisting of SEQ ID NOs;
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8. A composition comprising a non-transcribable polynucleotide and a transfer agent, wherein said non-transcribable polynucleotide is from 18 to about 700 nucleotides in length and is at least 85% identical or at least 85% complementary to a PPG oxidase gene sequence, or to an RNA transcript of said PPG oxidase gene sequence, wherein said PPG oxidase gene sequence is selected from the group consisting of SEQ ID NOs:
- 1-5 and 8-71 and a polynucleotide fragment thereof, wherein said transfer agent conditions the surface of a plant for permeation by said non-transcribable polynucleotide, and whereby said plant treated with said composition has its growth, development, or reproductive ability suppressed or delayed or said plant is more sensitive to a PPG oxidase inhibitor herbicide or mitosis inhibitor herbicide as a result of said non-transcribable polynucleotide containing composition relative to a plant not treated with said composition.
- View Dependent Claims (9, 10, 11, 12, 13, 14, 27, 29)
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15. A method of reducing expression of a PPG oxidase gene in a plant comprising:
- external application to said plant of a composition comprising a non-transcribable polynucleotide and a transfer agent, wherein said non-transcribable polynucleotide is from 18 to about 700 nucleotides in length and is at least 85% identical or at least 85% complementary to a PPG oxidase gene sequence, or to an RNA transcript of said PPG oxidase gene sequence, wherein said PPG oxidase gene sequence is selected from the group consisting of SEQ ID NOs;
1-5 and 8-71 and a polynucleotide fragment thereof, wherein said transfer agent conditions the surface of said plant for permeation by said non-transcribable polynucleotide, and whereby said expression of said PPG oxidase gene is reduced relative to a plant in which the composition was not applied. - View Dependent Claims (16, 17)
- external application to said plant of a composition comprising a non-transcribable polynucleotide and a transfer agent, wherein said non-transcribable polynucleotide is from 18 to about 700 nucleotides in length and is at least 85% identical or at least 85% complementary to a PPG oxidase gene sequence, or to an RNA transcript of said PPG oxidase gene sequence, wherein said PPG oxidase gene sequence is selected from the group consisting of SEQ ID NOs;
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18. A method of identifying non-transcribable polynucleotides useful in modulating PPG oxidase gene expression when externally treating a plant comprising:
- a) providing a plurality of non-transcribable polynucleotides that are from 18 to about 700 nucleotides in length and are at least 85 percent identical or at least 85 percent complementary to a PPG oxidase gene sequence selected from the group consisting of SEQ ID NOs;
1-5 and 8-71;
b) externally treating said plant with one or more of said non-transcribable polynucleotides and a transfer agent; and
c) analyzing said plant or extract for modulation of PPG oxidase gene expression, wherein said transfer agent conditions the surface of said plant for permeation by said one or more of said non-transcribable polynucleotides, and whereby a plant treated with said one or more of said non-transcribable polynucleotides and said transfer agent has its growth, development, or reproductive ability suppressed or delayed or said plant is more sensitive to a PPG oxidase inhibitor herbicide or mitosis inhibitor herbicide as a result of said one or more of said non-transcribable polynucleotides and said transfer agent relative to a plant not treated with said one or more of said non-transcribable polynucleotides and said transfer agent. - View Dependent Claims (19, 20, 21)
- a) providing a plurality of non-transcribable polynucleotides that are from 18 to about 700 nucleotides in length and are at least 85 percent identical or at least 85 percent complementary to a PPG oxidase gene sequence selected from the group consisting of SEQ ID NOs;
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22. An agricultural chemical composition comprising an admixture of a non-transcribable polynucleotide, a PPG oxidase inhibitor herbicide, and a co-herbicide, wherein said non-transcribable polynucleotide is from 18 to about 700 nucleotides in length and is at least 85% identical or at least 85% complementary to a portion of a PPG oxidase gene sequence, or to a portion of an RNA transcript of said PPG oxidase gene sequence, wherein said PPG oxidase gene sequence is selected from the group consisting of SEQ ID NOs:
- 1-5 and 8-71 and a polynucleotide fragment thereof, and whereby a plant treated with said composition has its growth, development, or reproductive ability suppressed or delayed or said plant is more sensitive to said PPG oxidase inhibitor herbicide or a mitosis inhibitor herbicide as a result of said non-transcribable polynucleotide containing composition relative to a plant not treated with said composition.
- View Dependent Claims (23)
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24. An agricultural chemical composition comprising an admixture of a non-transcribable polynucleotide, a PPG oxidase inhibitor herbicide, and a pesticide, wherein said non-transcribable polynucleotide is from 18 to about 700 nucleotides in length and is at least 85% identical or at least 85% complementary to a portion of a PPG oxidase gene sequence, or to a portion of an RNA transcript of said PPG oxidase gene sequence, wherein said PPG oxidase gene sequence is selected from the group consisting of SEQ ID NOs:
- 1-5 and 8-71 and a polynucleotide fragment thereof, and whereby a plant treated with said composition has its growth, development, or reproductive ability suppressed or delayed or said plant is more sensitive to said PPG oxidase inhibitor herbicide or a mitosis inhibitor herbicide as a result of said non-transcribable polynucleotide containing composition relative to a plant not treated with said composition.
- View Dependent Claims (25)
Specification