T-cell vaccination with viral vectors via mechanical epidermal disruption
First Claim
1. A method for inducing or stimulating a T cell mediated immune response to an exogenous T cell antigen in epithelial tissues of a human subject comprisingadministering to the epithelial tissue of the subject a live, modified, non-replicating poxvirus expressing the antigen to cause a local infection in the epithelial skin tissue in an amount sufficient to cause the infected cells to express viral proteins, the exogenous T cell antigen and inflammatory factors and stimulate a T cell immune response in the lymph nodes to the exogenous T cell antigen,wherein the live, modified, poxvirus expressing the antigen is administered to epithelial skin tissue that is disrupted to penetrate the stratum corneum without penetrating the entire epidermis.
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Accused Products
Abstract
Attenuated, replication-deficient viruses such as vaccinia viruses are used to deliver an exogenous viral, bacterial, parastic or tumor antigen to an epidermal tissue such as the skin, lungs or gastrointestinal tract, which has been mechanically disrupted, in an amount effective to elicit or stimulate a cell mediated immune response. The epidermis may be mechanically disrupted prior to, at the same time, or immediately after the administration of the vaccine. The vaccine can be used to induce immunity against a pathogen, such as a virus, bacteria, or parasite, or against a cancer in a subject that has or is at risk of developing cancer.
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Citations
13 Claims
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1. A method for inducing or stimulating a T cell mediated immune response to an exogenous T cell antigen in epithelial tissues of a human subject comprising
administering to the epithelial tissue of the subject a live, modified, non-replicating poxvirus expressing the antigen to cause a local infection in the epithelial skin tissue in an amount sufficient to cause the infected cells to express viral proteins, the exogenous T cell antigen and inflammatory factors and stimulate a T cell immune response in the lymph nodes to the exogenous T cell antigen, wherein the live, modified, poxvirus expressing the antigen is administered to epithelial skin tissue that is disrupted to penetrate the stratum corneum without penetrating the entire epidermis.
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13. A kit for inducing or stimulating a T cell-mediated immune response to an exogenous T cell antigen in human epithelial tissues comprising
a device for delivering poxvirus to a human subject'"'"'s epidermal tissue, wherein the device penetrates the stratum corneum without penetrating the entire epidermis, and a live, modified, non-replicating poxvirus expressing an antigen in an amount sufficient to cause a local infection in the epithelial tissue, and stimulate a T cell immune response in the lymph nodes to the exogenous T cell antigen when administered to epidermal tissue.
Specification