Real time imaging during solid organ transplant

• US 9,421,280 B2
• Filed: 02/10/2014
• Issued: 08/23/2016
• Est. Priority Date: 04/26/2005
• Status: Active Grant

• Current Assignee: Stryker Corporation
• Original Assignee: Novadaq Technologies, Inc. (Stryker Corporation)
• Inventors: Mangat, Gurpreet, Brzozowski, Lukasz, Croft, Richard L.
• Primary Examiner(s): Jones, D L
• Application Number:
  US14/177,045
• Publication Number:
  US 20140308210A1
• Time in Patent Office: 925 Days
• Field of Search: 424/1.11, 424/1.65, 424/9.1, 424/9.2, 424/9.6, 600/431
• US Class Current: 1/1
• CPC Class Codes: A61K 49/0019 characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules
• CPC Class Codes: A61K 49/0032 Methine dyes, e.g. cyanine dyes
• CPC Class Codes: A61K 49/0034 Indocyanine green, i.e. ICG, cardiogreen
• CPC Class Codes: A61K 49/0041 Xanthene dyes, used in vivo, e.g. administered to a mice, e.g. rhodamines, rose Bengal in vivo G01N
• CPC Class Codes: A61K 49/0043 Fluorescein, used in vivo
• CPC Class Codes: A61K 49/0045 the fluorescent agent being a peptide or protein used for imaging or diagnosis in vivo

Abstract

A method of determining the patency of a donor organ or at least one vessel to a donor organ in a donor subject includes administering a fluorescent dye to the donor subject, applying a sufficient amount of energy to the vessel such that the fluorescent dye fluoresces, obtaining a fluorescent image of the donor organ or the vessel attached to a donor organ, and observing the image to determine if a fluorescent signal is continuous through the vessel. A continuous fluorescent signal in the donor organ or vessel indicates the respective donor organ or vessel is patent.

Claims

1. A method of determining the patency of at least one vessel which is attached to a donor organ in a donor subject comprising:

a. administering a fluorescent dye to the donor subject;

b. applying energy to the vessel such that the fluorescent dye fluoresces;

c. obtaining a fluorescent image of the vessel attached to a donor organ; and

d. observing the image to determine if a fluorescent signal is continuous through the vessel,

wherein a continuous fluorescent signal in the vessel indicates the vessel is patent, and wherein the fluorescent dye is a tricarbocyanine dye, fluorescein isothlocyanate, rhodamine, phycoerythrin, phycocyanin, allophycocyanin, o-phthaldehyde, fluorescamine, Rose Bengal, trypan blue, fluoro-gold, or a combination thereof.

3. The method of claim 1, wherein the donor organ is a heart, a lung, a liver, a kidney, or a pancreas.

4. The method of claim 1, wherein the fluorescent dye is administered as a bolus injection.

5. The method of claim 1, wherein the tricarbocyanine dye is indocyanine green.

6. The method of claim 1, wherein the energy is provided by a laser.

7. The method of claim 1, wherein the energy is provided by a light emitting diode.

8. The method of claim 1, wherein the energy comprises infrared light.

9. The method of claim 1, wherein the fluorescent image is obtained with a surgical microscope or an endoscope.

10. The method of claim 1, wherein the image is obtained by a charge coupled device.

11. The method of claim 1, wherein the image is obtained by a video camera.

2. A method of determining the patency of a donor organ comprising:

a. administering a fluorescent dye to the vessel attached to the donor organ;

b. applying energy to the vessel such that the fluorescent dye fluoresces;

c. obtaining a fluorescent image of the donor organ; and

d. observing the image to determine if a fluorescent signal is continuous through the donor organ,

wherein continuous fluorescent signal in the donor organ indicates the donor organ is patent, and wherein the fluorescent dye is a tricarbocyanine dye, fluorescein isothlocyanate, rhodamine, phycoerythrin, phycocyanin, allophycocyanin, o-phthaldehyde, fluorescamine, Rose Bengal, trypan blue, fluoro-gold, or a combination thereof.

12. The method of claim 2, wherein the donor organ is a heart, a lung, a liver, a kidney, or a pancreas.

13. The method of claim 2, wherein the fluorescent dye is administered as a bolus injection.

14. The method of claim 2, wherein the tricarbocyanine dye is indocyanine green.

15. The method of claim 2, wherein the energy is provided by a laser.

16. The method of claim 2, wherein the energy is provided by a light emitting diode.

17. The method of claim 2, wherein the energy comprises infrared light.

18. The method of claim 2, wherein the fluorescent image is obtained with a surgical microscope or an endoscope.

19. The method of claim 2, wherein the image is obtained by a charge coupled device.

20. The method of claim 2, wherein the image is obtained by a video camera.