Reductive release probes containing a chemoselectively cleavable α-azidoether linker and methods of use thereof
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Abstract
Probes comprising one or more selectively cleavable α-azidoether moieties are provided; and linkers comprising the one or more selectively cleavable α-azidoether moieties. The α-azidoether moiety will undergo a Staudinger reaction with a suitable reducing agent, resulting in cleavage. The probes find use in a variety of detection assays, e.g. specific polynucleotide binding assays, polypeptide binding assays, etc. The cleavable linkers are suitable for synthetic reactions, e.g. to prepare probes of the invention; in the synthesis of cleavable peptide conjugates; and the like.
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16 Claims
- 1. A selectively cleavable probe of structure (I):
- 3. A selectively cleavable probe of one of structures (VIIIa) and (VIIIb):
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