System and method for sorting cells
First Claim
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1. A method of isolating desired cells comprising the steps of:
- a) supplying sperm cells to a nozzle of a flow cytometer;
b) supplying a sheath fluid comprising a citrate to the nozzle;
wherein the citrate is added to the sheath fluid prior to supplying the sheath fluid to the nozzle;
c) producing a stream containing the sperm cells from the nozzle;
d) sensing a property of sperm cells in the stream, the sensed property being indicative of a sex characteristic;
e) identifying X-chromosome bearing and/or Y-chromosome bearing sperm cells in the stream; and
f) collecting said X-chromosome bearing and/or Y-chromosome bearing sperm cells.
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Abstract
Improved flow cytometer system particularly adapted to use for sex-selected sperm sorting include enhanced sheath fluid and other strategies which minimize stress on the sperm cells, including a 2.9 percent sodium citrate sheath solution for bovine species and a hepes bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial applications of sperms samples as well as the resulting animals may be achieved.
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Citations
7 Claims
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1. A method of isolating desired cells comprising the steps of:
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a) supplying sperm cells to a nozzle of a flow cytometer; b) supplying a sheath fluid comprising a citrate to the nozzle;
wherein the citrate is added to the sheath fluid prior to supplying the sheath fluid to the nozzle;c) producing a stream containing the sperm cells from the nozzle; d) sensing a property of sperm cells in the stream, the sensed property being indicative of a sex characteristic; e) identifying X-chromosome bearing and/or Y-chromosome bearing sperm cells in the stream; and f) collecting said X-chromosome bearing and/or Y-chromosome bearing sperm cells. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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Specification