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System and method for sorting cells

  • US 9,422,523 B2
  • Filed: 02/11/2013
  • Issued: 08/23/2016
  • Est. Priority Date: 12/31/1997
  • Status: Expired due to Fees
First Claim
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1. A method of isolating desired cells comprising the steps of:

  • a) supplying sperm cells to a nozzle of a flow cytometer;

    b) supplying a sheath fluid comprising a citrate to the nozzle;

    wherein the citrate is added to the sheath fluid prior to supplying the sheath fluid to the nozzle;

    c) producing a stream containing the sperm cells from the nozzle;

    d) sensing a property of sperm cells in the stream, the sensed property being indicative of a sex characteristic;

    e) identifying X-chromosome bearing and/or Y-chromosome bearing sperm cells in the stream; and

    f) collecting said X-chromosome bearing and/or Y-chromosome bearing sperm cells.

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