Method for creating an internal transport system within tissue scaffolds using computer-aided tissue engineering

• US 9,427,496 B2
• Filed: 02/21/2006
• Issued: 08/30/2016
• Est. Priority Date: 02/18/2005
• Status: Active Grant

• Current Assignee: Drexel University
• Original Assignee: Drexel University
• Inventors: Sun, Wei, Nam, Jae Hyun
• Primary Examiner(s): NGUYEN, TUAN VAN
• Application Number:
  US11/358,358
• Publication Number:
  US 20060195179A1
• Time in Patent Office: 3,843 Days
• Field of Search: 424423-426, 424/93.7, 424443-444, 424/484, 424/486, 435/399, 435174-182, 435/397, 623 2372- 2376, 606/152
• US Class Current: 1/1
• CPC Class Codes: A61F 2/02 Prostheses implantable into the body closure means for urethra or rectum or for artificial body openings therefor A61F2/0004
• CPC Class Codes: A61F 2240/00 Manufacturing or designing of prostheses classified in groups A61F2/00 - A61F2/26 or A61F2/82 or A61F9/00 or A61F11/00 or subgroups thereof
• CPC Class Codes: A61L 27/38 containing added animal cells organs or tissue containing native cells A61L27/36
• CPC Class Codes: A61L 27/46 with phosphorus-containing inorganic fillers
• CPC Class Codes: A61L 27/48 with macromolecular fillers
• CPC Class Codes: A61L 27/52 Hydrogels or hydrocolloids

Abstract

An artificial tissue including an internal mass transport network having a plurality of channels, wherein the channels are designed to substantially mimic naturally occurring vascular network and a method for creating an internal transport system within a tissue scaffold to improve circulation, diffusion, and mass transport properties by utilizing computer-aided tissue engineering (CATE). The artificial tissue has the internal mass transport network of channels embedded, deposited, or molded within a scaffold, wherein the channels are made from a biodegradable transporting material and the scaffold is made from a scaffold material. The artificial tissue of the invention includes a basic circulatory system embedded within the tissue scaffold. This system provides mass transport throughout the entire scaffold and degrades after the new circulatory system develops.

Claims

1. An artificial tissue comprising an internal mass transport network having a plurality of channels, wherein the internal mass transport network comprises a biodegradable transporting material that is substantially free of suspended cells, and which is simultaneously deposited in a pattern with a scaffold material to form a plurality of layers of the artificial tissue, and wherein the channels are designed to substantially mimic a naturally occurring vascular network, such that the internal mass transport network creates a basic circulatory system within the scaffold, and wherein the internal mass transport network having a plurality of channels comprises the void space remaining after the biodegradable transporting material has biodegraded.

2. The artificial tissue of claim 1, wherein the biodegradable transporting material comprises a hydrogel and the scaffold material comprises a polymer and/or a bioactive glass.

3. The artificial tissue of claim 2, wherein the hydrogel is a member selected from the group consisting of alginate, collagen, chitosan, fibrin, hyaluronic acid, agar, polyethylene glycol and its copolymers, acrylamide-based polymers, acrylic acid-based polymers, and the scaffold material is a member selected from the group consisting of polycaproactone, polyglycolic acid, polylactic acid, polyhydroxybutyrate, polypropylene and their co-polymers, fumarate tricalcium phosphate, and hydroxyapatite.

4. The artificial tissue of claim 1, wherein the biodegradable transporting material is coated with a coating to form at least one coated channel or at least one partially coated channel such that a property of the at least one coated channel or the at least one partially coated channel is different from a property of a channel, wherein the property is selected from the group consisting of selective permeability, diffusivity, cell transport, cell adhesion, hydrophobicity, and a hydrophilicity.

5. The artificial tissue of claim 4, wherein the coating forms at least one barrier to affect cells migration.

6. The artificial tissue of claim 1, wherein the scaffold comprises more than one scaffold material.

7. The artificial tissue of claim 1, further comprising cells in contact with said scaffold.

8. The artificial tissue of claim 1, wherein the artificial tissue further comprises an artificial transpiration circulatory system connected to an outer layer of the artificial tissue, wherein the artificial transpiration circulatory system is adapted to aid circulation within the artificial tissue.