Compositions for isolating a target analyte from a heterogeneous sample

• US 9,428,547 B2
• Filed: 04/21/2011
• Issued: 08/30/2016
• Est. Priority Date: 04/21/2010
• Status: Active Grant

• Current Assignee: DNAE Group Holdings Limited (Genting Bhd.)
• Original Assignee: DNA Electronics, Inc. (Genting Bhd.)
• Inventors: Dryga, Sergey A., Esch, Victor C., Clarizia, Lisa-Jo Ann, Adams, Eddie W., Ung, Thearith H., Sitdikov, Ravil A.
• Primary Examiner(s): Hines, Ja'Na
• Application Number:
  US13/091,506
• Publication Number:
  US 20110263833A1
• Time in Patent Office: 1,958 Days
• US Class Current: 1/1
• CPC Class Codes: C07K 1/22 Affinity chromatography or related techniques based upon selective absorption processes
• CPC Class Codes: C07K 16/1267 from Gram-positive bacteria
• CPC Class Codes: C12N 13/00 Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 2563/143 Magnetism, e.g. magnetic label
• CPC Class Codes: C12Q 2565/629 being a microfluidic device
• CPC Class Codes: G01N 33/54333 Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction
• CPC Class Codes: G01N 33/56911 Bacteria

Abstract

The invention generally relates to compositions that include sets of magnetic particles, members of each set being conjugated to an antibody specific for a pathogen.

Claims

1. A composition for isolating pathogen from a heterogeneous sample, the composition comprising:

a plurality of sets of magnetic particles, members of different sets being conjugated to different antibodies that are specific for a pathogen, wherein at least two of the sets are at different concentrations; and

a buffer that substantially prevents lysis of blood cells and reduces particle aggregation.

2. The composition of claim 1, wherein each set is present at a concentration designed for detection of a specific pathogen in said sample.

3. The composition of claim 1, wherein the particles are differently labeled.

4. The composition of claim 3, wherein the label is an optical label.

5. The composition of claim 4, wherein the optical label is a fluorescent label.

6. The composition of claim 1, wherein the antibodies are monoclonal antibodies.

7. The composition of claim 1, wherein the antibodies are polyclonal antibodies.

8. The composition of claim 1, wherein the pathogen are gram positive bacteria.

9. The composition of claim 1, wherein the pathogen are gram negative bacteria.

10. The composition of claim 1, wherein the pathogen are gram positive and gram negative bacteria.

11. A plurality of magnetic particles within a buffer that substantially prevents lysis of blood cells and reduces particle aggregation, wherein the plurality is differently functionalized so that the plurality of particles bind to a plurality of different pathogens in a heterogeneous sample, wherein at least some of the differently functionalized particles are at different concentrations.

12. The particles of claim 11, wherein the particles are differently labeled.

13. The particles of claim 12, wherein the label is an optical label.

14. The particles of claim 13, wherein the optical label is a fluorescent label.

15. The particles of claim 11, wherein the antibodies are monoclonal antibodies.

16. The particles of claim 11, wherein the antibodies are polyclonal antibodies.

17. The particles of claim 11, wherein the pathogen are gram positive bacteria.

18. The particles of claim 11, wherein the pathogen are gram negative bacteria.

19. The particles of claim 11, wherein the pathogen are gram positive and gram negative bacteria.

20. A composition for isolating bacteria from a heterogeneous sample, the composition comprising:

a plurality of sets of magnetic particles, members of different sets being conjugated to different antibodies that are specific for a bacteria, wherein at least two of the sets are at different concentrations; and

a buffer that substantially prevents lysis of blood cells and reduces particle aggregation.