Oscillating amplification reaction for nucleic acids
First Claim
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1. A method for amplifying a template of nucleic acid target sequence contained in a sample comprising:
- contacting the sample with an amplification reaction mixture containing a primer complementary to the template of nucleic acid target sequence;
oscillating a temperature of the reaction between an upper temperature and a lower temperature wherein a change in temperature is no greater than 20°
C. during a plurality of temperature cycles; and
amplifying the template of nucleic acid target sequence.
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Abstract
One embodiment of the present invention provides for a method for amplifying a template of nucleic acid target sequence contained in a sample. The method includes contacting the sample with an amplification reaction mixture containing a primer complementary to the template of nucleic acid target sequence. A temperature of the reaction is oscillated between an upper temperature and a lower temperature wherein the change in temperature is no greater than about 20° C. during a plurality of temperature cycles. The template of nucleic acid target sequence is amplified.
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Citations
49 Claims
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1. A method for amplifying a template of nucleic acid target sequence contained in a sample comprising:
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contacting the sample with an amplification reaction mixture containing a primer complementary to the template of nucleic acid target sequence; oscillating a temperature of the reaction between an upper temperature and a lower temperature wherein a change in temperature is no greater than 20°
C. during a plurality of temperature cycles; andamplifying the template of nucleic acid target sequence. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 18, 19, 24, 25, 26, 30, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48)
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15. A method for amplifying a template of nucleic acid target sequence contained in a sample comprising:
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contacting the sample with an amplification reaction mixture comprising; a primer or a primer pair having a length of between 35-70 base pairs and complementary to the template of the nucleic acid target sequence and wherein the melting temperature of each primer of the primer pair is between 70-80°
C.;DMSO; monovalent cation; divalent cation; dNTPs; and DNA Polymerase; oscillating a temperature of the reaction between an upper temperature and a lower temperature wherein the change in temperature is no greater than about 20°
C. during a plurality of temperature cycles; andamplifying the template of nucleic acid target sequence. - View Dependent Claims (16, 17, 20, 21, 22, 23, 27, 28, 29, 31, 32, 49)
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Specification