Particle analyzer and particle analysis method
First Claim
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1. A flow cytometer comprising:
- a flow cell comprising an outlet, wherein the flow cell is configured to;
receive a cells-containing liquid and form a flow of the cells-containing liquid;
surround the cells-containing liquid with a sheath liquid to form a sample liquid; and
discharge the sample liquid at the outlet;
a light source that irradiates light onto the flow of the cells-containing liquid formed by the flow cell;
a photo-detector that detects optical information from cells in the flow of the cells-containing liquid and converts the optical information into an electric signal;
a temperature sensor configured to detect a temperature of the sample liquid, wherein the temperature sensor is arranged at the outlet of the flow cell such that the temperature sensor is configured to detect the temperature of the sample liquid after the flow of the cells-containing liquid is irradiated by the light source; and
a signal processing section that comprises a processor that executes instructions encoded on a computer readable medium that classifies cells and measures the classified cells on the basis of an output of the photo-detector and an output of the temperature sensor.
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Abstract
Particle analyzers are described that include a cell for receiving a particles-containing liquid; a light source for irradiating light onto the particles-containing liquid; a photo-detector for detecting optical information from particles in the particles-containing liquid and converting the optical information into an electric signal; a temperature sensor for detecting a temperature of the particles-containing liquid; and a signal processing section for calculating an analysis result of the particles on the basis of an output of the photo-detector and an output of the temperature sensor. Particle analysis methods are also described.
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Citations
25 Claims
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1. A flow cytometer comprising:
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a flow cell comprising an outlet, wherein the flow cell is configured to; receive a cells-containing liquid and form a flow of the cells-containing liquid; surround the cells-containing liquid with a sheath liquid to form a sample liquid; and discharge the sample liquid at the outlet; a light source that irradiates light onto the flow of the cells-containing liquid formed by the flow cell; a photo-detector that detects optical information from cells in the flow of the cells-containing liquid and converts the optical information into an electric signal; a temperature sensor configured to detect a temperature of the sample liquid, wherein the temperature sensor is arranged at the outlet of the flow cell such that the temperature sensor is configured to detect the temperature of the sample liquid after the flow of the cells-containing liquid is irradiated by the light source; and a signal processing section that comprises a processor that executes instructions encoded on a computer readable medium that classifies cells and measures the classified cells on the basis of an output of the photo-detector and an output of the temperature sensor. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 21, 22, 23, 24)
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11. A flow cytometer comprising:
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a flow cell for receiving a cells-containing liquid that forms a flow channel for the cells-containing liquid; a light source for irradiating the flow of the cells-containing liquid within the flow channel; a photo-detector optically linked to the flow channel that detects optical information from cells in the flow of the cells-containing liquid in the flow-channel and converts the optical information into an electric signal; a temperature sensor arranged to receive the cells-containing liquid after the cells-containing liquid has been irradiated by the light source for detecting a temperature of the cells-containing liquid after irradiation; and a processor that executes instructions retained on a computer readable medium that; analyzes the electric signal and thereby classifies the cells, and measures the classified cells as an analysis result; and corrects the electric signal obtained by the photo-detector or the analysis result obtained by the analysis of on an output of the temperature sensor. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18)
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19. A flow cytometer comprising:
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a flow cell for receiving a cells-containing liquid and for forming a flow of the cells-containing liquid; a light source for irradiating the flow of the cells-containing liquid; a photo-detector for detecting optical information from cells in the flow of the cells-containing liquid and for converting the optical information into an electric signal; a temperature sensor positioned to detect a temperature of the cells-containing liquid after the cells-containing liquid is irradiated by the light source; a variable gain amplifier that amplifies, based on the detected temperature, the electric signal, where the variable gain is based on the detected temperature; and an analyzing section that comprises a processor that executes instructions encoded on a computer readable medium that processes and analyzes the amplified electric signal and thereby classifies cells, and measures the classified cells based on the amplified electric signal.
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25. A flow cytometer comprising:
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a flow cell configured to receive a cells-containing liquid and for forming a flow of the cells-containing liquid in a first direction; a light source configured to irradiate light onto the flow of the cells-containing liquid formed by the flow cell; a photo-detector configured to detect optical information from cells in the flow of the cells-containing liquid and for converting the optical information into an electric signal; a temperature sensor configured to detect a temperature of the cells-containing liquid, wherein the temperature sensor is arranged at a position relative to the flow of the cells-containing liquid in the first direction that is after a position at which the cells-containing liquid is irradiated by the light source, wherein the position of the temperature sensor is such that the detected temperature of the cells-containing liquid is substantially the same as a temperature of the cells-containing liquid at a position where the cells-containing liquid is irradiated by the light source; and a signal processor that executes instructions retained in a computer readable medium that classifies cells on the basis of an output of the photo-detector and an output of the temperature sensor.
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Specification