Esterases useful in the treatment of cellulosic and lignocellulosic material

• US 9,434,929 B2
• Filed: 10/23/2013
• Issued: 09/06/2016
• Est. Priority Date: 10/26/2012
• Status: Active Grant

• Current Assignee: Roal Oy
• Original Assignee: Roal Oy
• Inventors: Jarvinen, Kristiina, Juntunen, Kari, Leinonen, Taija, Komander, Alexandra, Langfelder, Kim, Vehmaanpera, Jari, Puranen, Terhi
• Primary Examiner(s): Carlson, Karen Cochrane
• Application Number:
  US14/060,999
• Publication Number:
  US 20140120586A1
• Time in Patent Office: 1,049 Days
• US Class Current: 1/1
• CPC Class Codes: A21D 8/042 with enzymes
• CPC Class Codes: A23K 20/189 Enzymes
• CPC Class Codes: A23L 29/06 Enzymes
• CPC Class Codes: C11D 3/38636 containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
• CPC Class Codes: C12N 1/205 Bacterial isolates
• CPC Class Codes: C12N 9/18 Carboxylic ester hydrolases (3.1.1)
• CPC Class Codes: C12P 19/02 Monosaccharides 2-ketogulonic acid C12P7/60
• CPC Class Codes: C12R 2001/19 Escherichia coli
• CPC Class Codes: C12Y 301/01073 Feruloyl esterase (3.1.1.73)
• CPC Class Codes: Y02P 20/52 using catalysts, e.g. selective catalysts

Abstract

The present invention discloses novel polypeptides and enzyme preparations containing them, which improve the efficiency of cellulose and lignocellulose degradation even at elevated temperatures. The polypeptides can be produced using conventional recombinant DNA technologies. The related polynucleotides, vectors and host cells are also disclosed. The polypeptides and the enzyme preparations containing them are particularly useful in improving the efficiency of cellulose and lignocellulose degradation, in improving the quality of animal feed, in machine dishwashing applications, in detergent compositions, in pulp and paper, textile, food, baking or beverage industry.

Claims

1. A detergent composition comprising an enzyme preparation comprising a recombinant polypeptide having ferulic acid esterase activity and comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence of SEQ ID NO:

11 or SEQ ID NO;

12 in a spent culture medium, wherein the enzyme preparation further comprising additives.

2. The detergent composition according to claim 1, wherein the composition is a dishwashing machine cleaning composition.