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Installation of genomes or partial genomes into cells or cell-like systems

  • US 9,434,974 B2
  • Filed: 12/22/2006
  • Issued: 09/06/2016
  • Est. Priority Date: 12/23/2005
  • Status: Active Grant
First Claim
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1. A method for making a bacterial synthetic cell, the method comprising:

  • preparing a Mycoplasma capricolum recipient cell for installation of a bacterial genome;

    isolating a genome from a Mycoplasma mycoides cell by suspending the isolated genome in an agarose suspension; and

    introducing the isolated genome into the Mycoplasma capricolum recipient cell by contacting the recipient cell with the genome.wherein the genome of the Mycoplasma capricolum recipient cell is removed or inactivated to produce the recipient cell prior to, or after, introduction of the isolated genome by means of a physical, genetic or selective method or a combination thereof;

    wherein the introduced genome is self-replicating;

    the introduced genome does not recombine with a genome of the recipient cell; and

    the recipient cell takes on the phenotype programmed by the introduced genome thereby producing a synthetic cell.

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