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Methods and compositions for incorporating nucleotides

  • US 9,434,989 B2
  • Filed: 04/20/2015
  • Issued: 09/06/2016
  • Est. Priority Date: 03/19/2008
  • Status: Active Grant
First Claim
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1. A method for determining an identity of a nucleotide at a position in a nucleotide sequence, comprising:

  • a) providing primers, nucleic acid template molecules having a nucleotide sequence, polymerase, and a mixture of labeled and non-labeled reversible terminators, said labeled reversible terminators comprising a label linked to a nucleotide analogue, said label providing a signal, said signal having an intensity;

    b) incorporating, with a polymerase, a labeled reversible terminator from said mixture into a primer bound to a nucleic acid template molecule at a position corresponding to a complementary base in said template molecule;

    c) measuring the intensity of the signal of said label of said incorporated labeled reversible terminator; and

    d) determining the identity of the nucleotide at said position.

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