Method for processing lignocellulose containing material

• US 9,435,077 B2
• Filed: 12/10/2010
• Issued: 09/06/2016
• Est. Priority Date: 12/11/2009
• Status: Active Grant

• Current Assignee: Stichting Wageningen Research
• Original Assignee: Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno
• Inventors: Van Groenestijn, Johannes Wouterus, Jetten, Jan Matthijs, Van Deventer, Hendricus Cornelis, Slomp, Ronald, Slaghek, Theodoor Maximiliaan
• Primary Examiner(s): Visone, Thomas J
• Application Number:
  US13/514,944
• Publication Number:
  US 20120282660A1
• Time in Patent Office: 2,097 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: C08B 1/00 Preparatory treatment of cellulose for making derivatives thereof , e.g. pre-treatment, pre-soaking, activation
• CPC Class Codes: C08B 1/02 Rendering cellulose suitable for esterification esterification per se, C08B3/00, C08B5/00, C08B7/00 or C08B9/00
• CPC Class Codes: C08B 1/06 Rendering cellulose suitable for etherification etherification per se C08B11/00
• CPC Class Codes: C08H 1/00 Macromolecular products derived from proteins food proteins A23; glue, gelatine C09H
• CPC Class Codes: C08H 1/06 derived from horn, hoofs, hair, skin or leather
• CPC Class Codes: C08H 8/00 Macromolecular compounds derived from lignocellulosic materials pretreatment thereof B27N
• CPC Class Codes: C12P 2201/00 Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
• CPC Class Codes: C12P 2203/00 Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
• CPC Class Codes: C12P 7/10 substrate containing cellulosic material
• CPC Class Codes: D21B 1/021 by chemical means
• CPC Class Codes: D21B 1/12 by wet methods, by the use of steam beaters D21D1/00
• CPC Class Codes: D21C 1/02 with water or steam
• CPC Class Codes: D21C 1/04 with acid reacting compounds
• CPC Class Codes: D21C 1/06 with alkaline reacting compounds
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

The invention is related to a method for processing biomass derived from plants or animals, comprising the steps of:

• • a. pre treating said material with an aqueous solution of acid or base;
  • b. subsequently passaging saturated or super heated steam through said material,
    wherein the water activity of the process is controlled by means of temperature and pressure of the super heated steam to be less than 1, preferably less than 0.8, more preferably in the range of 0.4-0.8.

With such a process it is possible to disintegrate or make more accessible for subsequent treatments the lignocellulose from lignocellulose containing materials, like wood or other plant material, chitin from exoskeletons from Crustacea like crabs and shrimps, and proteins such as keratin from pig hair or chicken feather, for further derivation, like acylation, oxidation, etherification, carboxymethylation or esterification, or further enzymatic hydrolysis, and/or for production of chemicals, e.g. as sugars from carbohydrates for fermentation processes such as the production of (bio-) ethanolor as keratine hydrolysates for applications in paper or cosmetics.

Claims

1. A method for processing biomass derived from plants or animals to make available saccharides and proteins therein for further processing, comprising the steps of:

a. pre-treating said biomass material containing lignocellulose with an aqueous solution of acid or base to hydrolyze the saccharides from the lignocellulose or the proteins from the biomass material; and

b. subsequently passaging super heated “

SHS”

steam at an elevated pressure through a pressure chamber containing said pre-treated biomass material to further increase the separation of the saccharides from the lignocellulose or the proteins from the biomass material;

wherein the water activity “

Aw”

of the super heated steam is controlled by means of temperature and pressure of the super heated steam to be less than 1 and is defined by the formula

Aw=PSHS/P_{saturated steam} at T_SHS with P=absolute pressure,

and wherein the super heated steam is applied for at least 1 minute and less than 30 minutes.

2. The method according to claim 1, wherein the acid is sulfuric acid (H₂SO₄) or the base is chosen from the group consisting of calcium hydroxide, sodium hydroxide and potassium hydroxide, ammonium hydroxide or wherein the acid or base is any in situ formed acid or base.

3. The method according to claim 2, wherein the acid is provided in a solution of 0.1% to 4.0%.

4. The method according to claim 2, wherein the base is mixed with the biomass in a ratio of 0.02 to 0.2 grams base per gram biomass.

5. The method according to claim 1, wherein step a) is performed at a temperature of 20 to 80°

C.

6. The method according to claim 1, wherein the super heated steam is applied under a pressure between 1 and 10 bars.

7. The method according to claim 1, wherein the temperature of the super heated steam is between 150 and 220°

C.

8. The method according to claim 1, wherein the process comprises a further step c) of further enzymatic hydrolysis (exo and endo activity), acylation, oxidation, etherification, carboxymethylation or esterification of said material after super heated steam treatment.

9. The method according to claim 8, wherein step c) comprises acylation or carboxymethylation.

10. The method according to claim 1, wherein the material is woody plant material, including leaves, twigs, bark, grasses, hay, reeds, megasse, straw, wood chips, sawdust, bagasse, corn stover, corn cobs, wheat bran, sugar beet press cake, rice hulls, palm, coconut, cotton fibres and/or peat, sphagnum, filtercake from sewage plants, sewage effluents, animal waste, like feathers and hairs, or crystalline cellulose.

11. The method according to claim 1, wherein the saccharides are polysaccharides.

12. The method according to claim 11, wherein the process comprises a further step c) of applying an enzyme to process the polysaccharides into oligo- and monosaccharides.

13. The method according to claim 1, wherein the super heated steam is applied to the biomass for less than 15 minutes.

14. The method according to claim 13, wherein the super heated steam is applied to the biomass for 1 to 10 minutes.

16. The method according to claim 1, wherein the temperature of the super heated steam is between 160 and 220°

C.

19. The method according to claim 1, further comprising the step of:

c. opening said pressure chamber thereby allowing said pressure in said pressure chamber to return to atmospheric pressure and allowing said pre-treated biomass material within said pressure chamber to cool.

15. A method for processing biomass derived from plants or animals to make available saccharides and proteins therein for further processing, comprising the steps of:

a. pre-treating said biomass material containing lignocellulose with an aqueous solution of acid or base to hydrolyze the saccharides from the lignocellulose or the proteins from the biomass material; and

b. subsequently passaging super heated “

SHS”

steam at an elevated pressure through a pressure chamber containing said pre-treated biomass material to further increase the separation of the saccharides or the proteins from the biomass material;

wherein the water activity “

Aw”

of the super heated steam is controlled by means of temperature and pressure of the super heated steam to be less than 1 and is defined by the formula Aw=P_SHS/P_{saturated steam} at T_SHS with P=absolute pressure, and the temperature of the super heated steam is between 150 and 220°

C., and wherein the super heated steam is applied for at least 1 minute and less than 30 minutes.

17. The method according to claim 15, wherein the process further comprises step c) of further enzymatic hydrolysis (exo and endo activity), acylation, oxidation, etherification, carboxymethylation or esterification of said biomass material after super heated steam treatment.

18. The method according to claim 15, wherein the process further comprises step c) of applying an enzyme to said biomass material to process polysaccharides separated from said lignocellulose into oligo- and monosaccharides.

20. The method according to claim 15, further comprising the step of:

c. opening said pressure chamber thereby allowing said pressure in said pressure chamber to return to atmospheric pressure and allowing said pre-treated biomass material within said pressure chamber to cool.