Pluripotent cells
First Claim
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1. A method of transplanting progenitor cells to a mammalian host, comprising:
- (a) obtaining a population of isolated mammalian progenitor cells that co-express nestin and Oct-4 from fetal telencephalon and/or mesencephalon, and a culture medium that has a total calcium concentration of 0.03 to 0.15 mM, wherein the progenitor cells are suspended in the culture medium and wherein the progenitor cells continue to proliferate in an undifferentiated state for at least 4 months; and
(b) transplanting the progenitor cells to the mammalian host, wherein said progenitor cells further express a marker selected from the group consisting of TRA-1-60, TRA-1-81 and SSEA-4.
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Abstract
Pluripotent cells that are immunopositive for both the neural progenitor marker nestin and a pluripotent cell marker are provided. The cells exhibit rapid doubling times and can be maintained in vitro for extended periods. Also provided are cell cultures containing the pluripotent cells, a method of transplanting human pluripotent cells to a host, and a method of reducing seizure activity in a subject. These pluripotent cells, when transplanted into the ventricle of a host animal, migrate to the site of damage and adopt a suitably corrective phenotype, resulting in both structural and functional restoration.
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Citations
20 Claims
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1. A method of transplanting progenitor cells to a mammalian host, comprising:
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(a) obtaining a population of isolated mammalian progenitor cells that co-express nestin and Oct-4 from fetal telencephalon and/or mesencephalon, and a culture medium that has a total calcium concentration of 0.03 to 0.15 mM, wherein the progenitor cells are suspended in the culture medium and wherein the progenitor cells continue to proliferate in an undifferentiated state for at least 4 months; and (b) transplanting the progenitor cells to the mammalian host, wherein said progenitor cells further express a marker selected from the group consisting of TRA-1-60, TRA-1-81 and SSEA-4. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification