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Pluripotent cells

  • US 9,439,932 B2
  • Filed: 01/17/2013
  • Issued: 09/13/2016
  • Est. Priority Date: 12/02/2003
  • Status: Active Grant
First Claim
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1. A method of transplanting progenitor cells to a mammalian host, comprising:

  • (a) obtaining a population of isolated mammalian progenitor cells that co-express nestin and Oct-4 from fetal telencephalon and/or mesencephalon, and a culture medium that has a total calcium concentration of 0.03 to 0.15 mM, wherein the progenitor cells are suspended in the culture medium and wherein the progenitor cells continue to proliferate in an undifferentiated state for at least 4 months; and

    (b) transplanting the progenitor cells to the mammalian host, wherein said progenitor cells further express a marker selected from the group consisting of TRA-1-60, TRA-1-81 and SSEA-4.

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