Enzyme immobilization by crosslinking
First Claim
1. An analyte sensor comprising:
- a sensing layer in contact with a surface of an electrode, wherein the sensing layer comprises a first crosslinked, hydrophilic, diblock copolymer having an immobilized analyte sensing component, wherein the first diblock copolymer comprises a first block of poly(alkylene oxide) and a second block of poly(vinyl pyridine); and
a protective membrane comprising a second crosslinked, hydrophilic, diblock copolymer, wherein the second diblock copolymer comprises a poly(alkylene oxide) capped random copolymer of vinyl pyridine and styrene.
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Abstract
An analyte sensor for the continuous or semi-continuous monitoring of physiological parameters and a method for making the analyte sensor are disclosed. In one aspect, the analyte sensor includes an electrode, a sensing layer in contact with a surface of the electrode, and a protective membrane. The sensing layer is a crosslinked, hydrophilic copolymer including poly(alkylene oxide) and poly(vinyl pyridine), and an analyte sensing component is immobilized within the crosslinked, hydrophilic copolymer. The protective membrane is a crosslinked, hydrophilic copolymer including alkylene oxide, vinyl pyridine and styrene units. The method involves the formation of a sensing layer on a surface of an electrode, followed by the formation of a protective membrane on a surface of the sensing layer.
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Citations
19 Claims
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1. An analyte sensor comprising:
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a sensing layer in contact with a surface of an electrode, wherein the sensing layer comprises a first crosslinked, hydrophilic, diblock copolymer having an immobilized analyte sensing component, wherein the first diblock copolymer comprises a first block of poly(alkylene oxide) and a second block of poly(vinyl pyridine); and a protective membrane comprising a second crosslinked, hydrophilic, diblock copolymer, wherein the second diblock copolymer comprises a poly(alkylene oxide) capped random copolymer of vinyl pyridine and styrene. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A method for manufacturing an analyte sensor, the method comprising:
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forming a first solution of a first hydrophilic, diblock copolymer, a first crosslinking agent and an analyte sensing component, wherein the first hydrophilic, diblock copolymer comprises a first block of poly(alkylene oxide) and second block of poly(vinyl pyridine); depositing the first solution onto a surface of an electrode; curing the first solution to provide a sensing layer; forming a second solution of a second hydrophilic, diblock copolymer and a second crosslinking agent, wherein the second hydrophilic, diblock copolymer comprises a poly(alkylene oxide) capped random copolymer of vinyl pyridine and styrene; depositing the second solution onto a surface of the sensing layer; and curing the second solution to provide a protective membrane. - View Dependent Claims (10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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Specification