Optical coherence photoacoustic microscopy

• US 9,442,095 B2
• Filed: 06/15/2012
• Issued: 09/13/2016
• Est. Priority Date: 06/15/2011
• Status: Active Grant

• Current Assignee: Northwestern University, University of Southern California
• Original Assignee: Northwestern University, University of Southern California
• Inventors: Jiao, Shuliang, Zhang, Hao
• Primary Examiner(s): Geisel, Kara E
• Assistant Examiner(s): BOLOGNA, DOMINIC JOSEPH
• Application Number:
  US13/524,813
• Publication Number:
  US 20120320368A1
• Time in Patent Office: 1,551 Days
• US Class Current: 1/1
• CPC Class Codes: G01B 9/0203 With imaging systems
• CPC Class Codes: G01B 9/02091 Tomographic interferometers, e.g. based on optical coherence
• CPC Class Codes: G01N 29/0681 by acoustic microscopy, e.g. scanning acoustic microscopy
• CPC Class Codes: G01N 29/2418 using optoacoustic interaction with the material, e.g. laser radiation, photoacoustics photoacoustic cells G01N21/1702; measuring characteristics of vibrations by using radiation-sensitive means G01H9/00; acousto-optical conversion techniques for short-range imaging G01S15/8965; sound-producing devices using laser bundle G10K15/046

Abstract

A system and method for providing an optical coherence photoacoustic (OC-PAM) microscopy. An OC-PAM microscope includes a light source that outputs light, a scanner, a detector, a transducer, and an image processing module. The scanner receives the light and scans the light across a sample. The detector receives reflected light from the sample in response to the scanned light. The transducer detects photoacoustic waves induced in the sample by the scanned light. The image processing module receives output from the detector and the transducer and generates a photoacoustic microscopy (PAM) image and an optical coherence tomography (OCT) image based on the received output from the detector and the transducer. The PAM and OCT image data may be fused to form a single, OC-PAM image. Additionally, a series of PAM images and OCT images, respectively, may be combined to generate three-dimensional PAM and OCT images, respectively.

Claims

1. An optical coherence photoacoustic microscope comprising:

a light source that outputs light of a single center wavelength;

a scanner that scans the output light of the single center wavelength across a sample by moving at least one of the sample and the light relative to the other of the sample and the light;

a detector that receives reflected light from the sample in response to the scanned light;

a transducer positioned to detect photoacoustic waves induced in the sample by the scanned light; and

an image processing module that receives output from the transducer and the detector and that generates a photoacoustic microscopy (PAM) image and an optical coherence tomography (OCT) image based on the received output.

2. The optical coherence photoacoustic microscope of claim 1, wherein the light source is one of a pulsed broadband laser that outputs pulsed broadband light and a swept laser source that outputs pulsed swept laser light of a plurality of wavelengths, wherein the wavelengths are swept in a scan period shorter than 10 nanoseconds.

3. The optical coherence photoacoustic microscope of claim 1, wherein the image processing module further fuses together the PAM image and the OCT image to form a fused image.

4. The optical coherence photoacoustic microscope of claim 1, further comprising a beam splitter that splits the light into sample arm light and reference arm light.

5. The optical coherence photoacoustic microscope of claim 4, wherein the light received by the sample is the sample arm light.

6. The optical coherence photoacoustic microscope of claim 5, wherein the sample arm light passes through an optical system including a lens and a mirror.

7. The optical coherence photoacoustic microscope of claim 4, further comprising:

a plate that partially reflects and partially transmits the reference arm light; and

a photodiode that detects the reference arm light reflected by the plate and that outputs a trigger signal to initiate data acquisition.

8. The optical coherence photoacoustic microscope of claim 7, wherein the plate is a glass plate.

9. The optical coherence photoacoustic microscope of claim 7, wherein the reflected light received by the detector further includes the reference arm light transmitted by the plate.

10. The optical coherence photoacoustic microscope of claim 4, wherein the beam-splitter is one of fiber-based and free-space-based.

11. The optical coherence photoacoustic microscope of claim 1, wherein the detector includes

a spectrometer, which receives the reflected light from the sample in response to the scanned light;

and

a camera that captures the reflected light received by the spectrometer and provides the output to the image processing module.

12. The optical coherence photoacoustic microscope of claim 1, wherein the detector includes

a balanced photo-detector;

which receives the reflected light from the sample in response to the scanned light; and

a digitizer that captures the reflected light received by the balanced photo-detector and provides the output to the image processing module.

13. The optical coherence photoacoustic microscope of claim 1, wherein the light is scanned across the sample via an x-y scanner.

14. The optical coherence photoacoustic microscope of claim 1, wherein the transducer is positioned in a reflective configuration, whereby the transducer is on a laser receiving side of the sample.

15. The optical coherence photoacoustic microscope of claim 1, wherein the transducer is positioned in a transmission configuration, whereby the transducer is on a second side opposite the laser receiving side.

16. A method for optical coherence photoacoustic microscopy comprising:

emitting light of a single center wavelength from a light source;

scanning the light of the single center wavelength across a sample by moving at least one of the sample and the light relative to the other of the sample and the light;

receiving, by a detector, reflected light from the sample in response to the scanned light;

detecting, by a transducer, photoacoustic waves induced in the sample by the scanned light;

receiving, by an image processing module, output from the transducer and the detector; and

generating, by the image processing module, a photoacoustic microscopy (PAM) image and an optical coherence tomography (OCT) image based on the received output.

17. The method of claim 16, wherein the light source is one of a pulsed broadband laser that outputs pulsed broadband light and a swept laser source that outputs pulsed swept laser light of a plurality of wavelengths swept in a scan period shorter than 10 nanoseconds.

18. The method of claim 17, wherein the light source is the swept laser source and the detected photoacoustic waves have energy from the sum of the pulsed swept laser light of the plurality of wavelengths in the scan period.

19. The method of claim 16, further comprising fusing together the PAM image and the OCT image to form a fused image.

20. The method of claim 16, further comprising splitting, via a beam splitter, the light into sample arm light and reference arm light.

21. The method of claim 20, wherein the light scanned across the sample is the sample arm light.

22. The method of claim 20, further comprising transmitting the sample arm light through an optical system including a lens and a mirror on route to the sample.

23. The method of claim 20, further comprising:

partially reflecting and partially transmitting the reference arm light using a plate;

detecting, with a photodiode, the reference arm light reflected by the plate; and

outputting, with the photodiode, a trigger signal to initiate data acquisition.

24. The method of claim 23, further comprising combining the reflected light, before reaching the detector, with the reference arm light partially transmitted by the plate.

25. The method of claim 16, wherein the receiving, by the detector, includes

receiving, by a spectrometer, the reflected light from the sample in response to the scanned light;

and

capturing, by a camera, the reflected light received by the spectrometer.

26. The method of claim 16, wherein the receiving, by the detector, includes

receiving, by a balanced photo-detector;

the reflected light from the sample in response to the scanned light; and

capturing, by a digitizer, the reflected light received by the balanced photo-detector.

27. The method of claim 16, further comprising establishing a depth relationship between the PAM image and the OCT image.

28. The method of claim 16, wherein scanning the light across the sample includes scanning the light via an x-y scanner that directs the light.

29. The method of claim 16, further comprising positioning the transducer in a reflective configuration, whereby the transducer is on a laser receiving side of the sample.

30. The method of claim 16, further comprising positioning the transducer in a transmission configuration, whereby the transducer is on a second side opposite the laser receiving side.