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Method for the detection of biological molecules using a two particle complex

  • US 9,442,117 B2
  • Filed: 08/21/2014
  • Issued: 09/13/2016
  • Est. Priority Date: 06/23/2004
  • Status: Active Grant
First Claim
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1. A method of detecting an analyte of interest in a sample, comprising(a) forming a composition comprising:


  • (A)k (B)u,(C),(D)x wherein A is an electrogenerated chemiluminescent (ECL) moiety which can be induced to repeatedly emit electromagnetic radiation by direct exposure to an electrochemical energy source; and

    the ECL moiety is soluble in organic solvent and insoluble in aqueous solvent;

    B is a synthetic organic polymer bead comprising a plurality of the ECL moieties entrapped within its interior; and

    B is either linked to the analyte of interest or linked to a first specific binding partner of the analyte of interest;

    C is the sample which may contain the analyte of interest; and

    D is a magnetizable bead which is either linked to the analyte of interest or linked to a second specific binding partner of the analyte of interest;

    wherein k, u, and x are each an integer equal to or greater than 1;

    (b) separating a complex comprising A, B, D and the analyte of interest from other components of the composition;

    (c) inducing the ECL moiety in the complex to repeatedly emit electromagnetic radiation by directly exposing the moiety to electrochemical energy; and

    (d) detecting the emitted electromagnetic radiation and thereby detecting the presence of the analyte of interest,provided that B and D are not both linked to the analyte of interest.

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